Differences in Bacterial Small RNAs in Stool Samples from Hypercholesterolemic and Normocholesterolemic Subjects

Primer Autor
Saavedra, Nicolas
Co-autores
Morales, Cristian
Arias-Carrasco, Raul
Maracaja-Coutinho, Vinicius
Seron, Pamela
Lanas, Fernando
Salazar, Luis A.
Título
Differences in Bacterial Small RNAs in Stool Samples from Hypercholesterolemic and Normocholesterolemic Subjects
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
Cholesterol metabolism is important at the physiological level as well as in several diseases, with small RNA being an element to consider in terms of its epigenetic control. Thus, the aim of this study was to identify differences between bacterial small RNAs present at the gut level in hypercholesterolemic and normocholesterolemic individuals. Twenty stool samples were collected from hypercholesterolemic and normocholesterolemic subjects. RNA extraction and small RNA sequencing were performed, followed by bioinformatics analyses with BrumiR, Bowtie 2, BLASTn, DESeq2, and IntaRNA, after the filtering of the reads with fastp. In addition, the prediction of secondary structures was obtained with RNAfold WebServer. Most of the small RNAs were of bacterial origin and presented a greater number of readings in normocholesterolemic participants. The upregulation of small RNA ID 2909606 associated with Coprococcus eutactus (family Lachnospiraceae) was presented in hypercholesterolemic subjects. In addition, a positive correlation was established between small RNA ID 2149569 from the species Blautia wexlerae and hypercholesterolemic subjects. Other bacterial and archaeal small RNAs that interacted with the LDL receptor (LDLR) were identified. For these sequences, the prediction of secondary structures was also obtained. There were significant differences in bacterial small RNAs associated with cholesterol metabolism in hypercholesterolemic and normocholesterolemic participants.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/ijms24087213
Formato Recurso
PDF
Palabras Claves
bacterial small RNAs
gut microbiota
cholesterol metabolism
small RNA sequencing
Ubicación del archivo
http://dx.doi.org/10.3390/ijms24087213
Categoría OCDE
Bioquímica y biología molecular
Química
Materias
pequeños ARN bacterianos
microbiota intestinal
metabolismo del colesterol
secuenciación de ARN pequeño
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000977741300001
Tipo de ruta
verde# dorado
Categoría WOS
Bioquímica y biología molecular
Química
Colecciones
Colección Publicaciones Científicas
Colección ANID
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