Repositorio UFRO · Omeka S

Differences in Bacterial Small RNAs in Stool Samples from Hypercholesterolemic and Normocholesterolemic Subjects

Primer Autor	Saavedra, Nicolas
Co-autores	Morales, Cristian Arias-Carrasco, Raul Maracaja-Coutinho, Vinicius Seron, Pamela Lanas, Fernando Salazar, Luis A.
Título	Differences in Bacterial Small RNAs in Stool Samples from Hypercholesterolemic and Normocholesterolemic Subjects
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje	en
Resumen	Cholesterol metabolism is important at the physiological level as well as in several diseases, with small RNA being an element to consider in terms of its epigenetic control. Thus, the aim of this study was to identify differences between bacterial small RNAs present at the gut level in hypercholesterolemic and normocholesterolemic individuals. Twenty stool samples were collected from hypercholesterolemic and normocholesterolemic subjects. RNA extraction and small RNA sequencing were performed, followed by bioinformatics analyses with BrumiR, Bowtie 2, BLASTn, DESeq2, and IntaRNA, after the filtering of the reads with fastp. In addition, the prediction of secondary structures was obtained with RNAfold WebServer. Most of the small RNAs were of bacterial origin and presented a greater number of readings in normocholesterolemic participants. The upregulation of small RNA ID 2909606 associated with Coprococcus eutactus (family Lachnospiraceae) was presented in hypercholesterolemic subjects. In addition, a positive correlation was established between small RNA ID 2149569 from the species Blautia wexlerae and hypercholesterolemic subjects. Other bacterial and archaeal small RNAs that interacted with the LDL receptor (LDLR) were identified. For these sequences, the prediction of secondary structures was also obtained. There were significant differences in bacterial small RNAs associated with cholesterol metabolism in hypercholesterolemic and normocholesterolemic participants.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/ijms24087213
Formato Recurso	PDF
Palabras Claves	bacterial small RNAs gut microbiota cholesterol metabolism small RNA sequencing
Ubicación del archivo	http://dx.doi.org/10.3390/ijms24087213
Categoría OCDE	Bioquímica y biología molecular Química
Materias	pequeños ARN bacterianos microbiota intestinal metabolismo del colesterol secuenciación de ARN pequeño
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000977741300001
Tipo de ruta	verde# dorado
Categoría WOS	Bioquímica y biología molecular Química