Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis

Primer Autor
Ortega, Ana
Co-autores
Flores-Chova, Ana
Martinez-Arroyo, Olga
Riffo-Campos, Angela L. L.
Forner, Maria J. J.
Cortes, Raquel
Título
Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
Despite considerable progress in our understanding of systemic lupus erythematosus (SLE) pathophysiology, patient diagnosis is often deficient and late, and this has an impact on disease progression. The aim of this study was to analyze non-coding RNA (ncRNA) packaged into exosomes by next-generation sequencing to assess the molecular profile associated with renal damage, one of the most serious complications of SLE, to identify new potential targets to improve disease diagnosis and management using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The plasma exosomes had a specific ncRNA profile associated with lupus nephritis (LN). The three ncRNA types with the highest number of differentially expressed transcripts were microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and piwi-interacting RNAs (piRNAs). We identified an exosomal 29-ncRNA molecular signature, of which 15 were associated only with LN presence, piRNAs were the most representative, followed by lncRNAs and miRNAs. The transcriptional regulatory network showed a significant role for four lncRNAs (LINC01015, LINC01986, AC087257.1 and AC022596.1) and two miRNAs (miR-16-5p and miR-101-3p) in network organization, targeting critical pathways implicated in inflammation, fibrosis, epithelial-mesenchymal transition and actin cytoskeleton. From these, a handful of potential targets, such as transforming growth factor-beta (TGF-beta) superfamily binding proteins (activin-A, TGFB receptors, etc.), WNT/beta-catenin and fibroblast growth factors (FGFs) have been identified for use as therapeutic targets of renal damage in SLE.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/ijms24087088
Formato Recurso
PDF
Palabras Claves
systemic lupus erythematosus
exosomes
non-coding RNA
RNA sequencing
bioinformatics enrichment analysis
Ubicación del archivo
Categoría OCDE
Bioquímica y biología molecular
Química
Materias
lupus eritematoso sistémico
exosomas
ARN no codificante
secuenciación de ARN
análisis de enriquecimiento bioinformático
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:000979318900001
Tipo de ruta
verde# dorado
Categoría WOS
Bioquímica y biología molecular
Química
Referencia del Financiador (Mandatado si es aplicable-repetible)
ISCIII PI18/01405
ISCIII PI21/00249
FI20/00096
FI22/00032
MICINN PID2020-117114GB-I00
ANID NCS2021_013
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