Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis
Primer Autor |
Ortega, Ana
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Co-autores |
Flores-Chova, Ana
Martinez-Arroyo, Olga
Riffo-Campos, Angela L. L.
Forner, Maria J. J.
Cortes, Raquel
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Título |
Plasma Exosomal Non-Coding RNA Profile Associated with Renal Damage Reveals Potential Therapeutic Targets in Lupus Nephritis
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Editorial |
MDPI
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Revista |
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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Lenguaje |
en
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Resumen |
Despite considerable progress in our understanding of systemic lupus erythematosus (SLE) pathophysiology, patient diagnosis is often deficient and late, and this has an impact on disease progression. The aim of this study was to analyze non-coding RNA (ncRNA) packaged into exosomes by next-generation sequencing to assess the molecular profile associated with renal damage, one of the most serious complications of SLE, to identify new potential targets to improve disease diagnosis and management using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The plasma exosomes had a specific ncRNA profile associated with lupus nephritis (LN). The three ncRNA types with the highest number of differentially expressed transcripts were microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and piwi-interacting RNAs (piRNAs). We identified an exosomal 29-ncRNA molecular signature, of which 15 were associated only with LN presence, piRNAs were the most representative, followed by lncRNAs and miRNAs. The transcriptional regulatory network showed a significant role for four lncRNAs (LINC01015, LINC01986, AC087257.1 and AC022596.1) and two miRNAs (miR-16-5p and miR-101-3p) in network organization, targeting critical pathways implicated in inflammation, fibrosis, epithelial-mesenchymal transition and actin cytoskeleton. From these, a handful of potential targets, such as transforming growth factor-beta (TGF-beta) superfamily binding proteins (activin-A, TGFB receptors, etc.), WNT/beta-catenin and fibroblast growth factors (FGFs) have been identified for use as therapeutic targets of renal damage in SLE.
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Fecha Publicación |
2023
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Tipo de Recurso |
artículo original
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doi |
10.3390/ijms24087088
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Formato Recurso |
PDF
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Palabras Claves |
systemic lupus erythematosus
exosomes
non-coding RNA
RNA sequencing
bioinformatics enrichment analysis
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Ubicación del archivo | |
Categoría OCDE |
Bioquímica y biología molecular
Química
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Materias |
lupus eritematoso sistémico
exosomas
ARN no codificante
secuenciación de ARN
análisis de enriquecimiento bioinformático
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Identificador del recurso (Mandatado-único) |
artículo original
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Versión del recurso (Recomendado-único) |
versión publicada
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License |
CC BY 4.0
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Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
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Derechos de acceso |
acceso abierto
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Access Rights |
acceso abierto
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Id de Web of Science |
WOS:000979318900001
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Tipo de ruta |
verde# dorado
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Categoría WOS |
Bioquímica y biología molecular
Química
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Referencia del Financiador (Mandatado si es aplicable-repetible) |
ISCIII PI18/01405
ISCIII PI21/00249
FI20/00096
FI22/00032
MICINN PID2020-117114GB-I00
ANID NCS2021_013
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