Repositorio UFRO · Omeka S

Biological Properties of Dental Pulp Stem Cells Isolated from Inflamed and Healthy Pulp and Cultured in an Inflammatory Microenvironment

Primer Autor	Bucchi, Cristina
Co-autores	Bucchi, Ana Martinez-Rodriguez, Paulina
Título	Biological Properties of Dental Pulp Stem Cells Isolated from Inflamed and Healthy Pulp and Cultured in an Inflammatory Microenvironment
Editorial	ELSEVIER SCIENCE INC
Revista	JOURNAL OF ENDODONTICS
Lenguaje	en
Resumen	Introduction: The aim of this study was to assess whether the biological characteristics of dental pulp stem cells (DPSCs), such as viability, adhesion to dentin, mineralization, and release of immunomodulatory cytokines, are affected by the inflammatory status of the donor tissue and/or the sustained inflammatory environment. Methods: DPSCs were isolated from pulps from 3 caries-free teeth (healthy or hDPSCs), and from 3 teeth with irreversible pulpitis or deep caries (unhealthy DPSCs or uDPSCs). The cells were cultured in odontogenic and osteogenic media with or without lipopolysaccharides. Viability was analyzed by MTT assay at days 1, 3, 5, and 7, adhesion to dentin was evaluated through an environmental scanning electron microscope after 48 hours and through MTT assay, mineralization was analyzed with alizarin red staining after 21 days, and the release of proinflammatory (interleukin 6) and immunosuppressive cytokines (interleukin 10) was measured with the enzyme-linked immunosorbent assay after 24 hours and 7 days. Results: The inflammatory status of the pulp significantly reduced the viability and mineralization capacity of the DPSCs, although it did not affect the adhesion capacity to dentin or the secretion of the proinflammatory interleukin. The inflammatory microenvironment (lipopolysaccharide) only had a significant impact on the secretion of interleukin 6, which was augmented after 7 days. Conclusions: The inflammatory status of the dental pulp should be taken into account when the use of DPSCs is intended either for research and/or for application in reparative or regenerative therapies.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.joen.2023.02.002
Formato Recurso	PDF
Palabras Claves	Dental pulp stem cells inflammation lipopolysaccharide
Ubicación del archivo	http://dx.doi.org/10.1016/j.joen.2023.02.002
Categoría OCDE	Odontología, Cirugía Bucal y Medicina
Materias	Células madre de pulpa dental inflamación lipopolisacárido
Página de inicio (Recomendado-único)	395.0
Página final (Recomendado-único)	+
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	metadata
Access Rights	metadata
Id de Web of Science	WOS:001047481600001
ISSN	0099-2399
Categoría WOS	Odontología, Cirugía Bucal y Medicina
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID FONDECYT 11200023