Metabologenomics analysis of Pseudomonas sp. So3.2b, an Antarctic strain with bioactivity against Rhizoctonia solani
| Primer Autor |
Barrientos, Leticia
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| Co-autores |
Maimone, Naydja Moralles
Pozza Jr, Mario Cezar
de Oliveira, Lucianne Ferreira Paes
Rojas-Villalta, Dorian
de Lira, Simone Possedente
Nunez-Montero, Kattia
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| Título |
Metabologenomics analysis of Pseudomonas sp. So3.2b, an Antarctic strain with bioactivity against Rhizoctonia solani
|
| Editorial |
FRONTIERS MEDIA SA
|
| Revista |
FRONTIERS IN MICROBIOLOGY
|
| Lenguaje |
en
|
| Resumen |
IntroductionPhytopathogenic fungi are a considerable concern for agriculture, as they can threaten the productivity of several crops worldwide. Meanwhile, natural microbial products are acknowledged to play an important role in modern agriculture as they comprehend a safer alternative to synthetic pesticides. Bacterial strains from underexplored environments are a promising source of bioactive metabolites. MethodsWe applied the OSMAC (One Strain, Many Compounds) cultivation approach, in vitro bioassays, and metabolo-genomics analyses to investigate the biochemical potential of Pseudomonas sp. So3.2b, a strain isolated from Antarctica. Crude extracts from OSMAC were analyzed through HPLC-QTOF-MS/MS, molecular networking, and annotation. The antifungal potential of the extracts was confirmed against Rhizoctonia solani strains. Moreover, the whole-genome sequence was studied for biosynthetic gene clusters (BGCs) identification and phylogenetic comparison. Results and DiscussionMolecular networking revealed that metabolite synthesis has growth media specificity, and it was reflected in bioassays results against R. solani. Bananamides, rhamnolipids, and butenolides-like molecules were annotated from the metabolome, and chemical novelty was also suggested by several unidentified compounds. Additionally, genome mining confirmed a wide variety of BGCs present in this strain, with low to no similarity with known molecules. An NRPS-encoding BGC was identified as responsible for producing the banamides-like molecules, while phylogenetic analysis demonstrated a close relationship with other rhizosphere bacteria. Therefore, by combining -omics approaches and in vitro bioassays, our study demonstrates that Pseudomonas sp. So3.2b has potential application to agriculture as a source of bioactive metabolites.
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| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
|
| doi |
10.3389/fmicb.2023.1187321
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| Formato Recurso |
PDF
|
| Palabras Claves |
bioactivity
OSMAC
molecular networking
secondary metabolites
genomics
biosynthetic gene cluster
bioprospecting
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| Ubicación del archivo | |
| Categoría OCDE |
Microbiología
|
| Materias |
bioactividad
OSMAC
redes moleculares
metabolitos secundarios
genómica
grupo de genes biosintéticos
bioprospección
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| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:000989926600001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Microbiología
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1210563
ANID-FONDECYT 11230475
INACH DG_01-19
NEXER NXR17-0003
TEC 5402-1510-1035
CONICYT-PFCHA 2017-21170263
CAPES 001
FAPESP 2019/17721-9
FAPESP 2022/01529-4
CNPQ 142260/2020-4
CNPEQ141501/2020-0
ANID FONDECYT 1210563
ANID FONDECYT 11230475
Instituto Tecnológico de Costa Rica 5402-1510-1035
ANID CONICYT-PFCHA 2017-21170263
CNPq 142260/2020-4
CNPq 141501/2020-0
|
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