Repositorio UFRO · Omeka S

Metabologenomics analysis of Pseudomonas sp. So3.2b, an Antarctic strain with bioactivity against Rhizoctonia solani

Primer Autor	Barrientos, Leticia
Co-autores	Maimone, Naydja Moralles Pozza Jr, Mario Cezar de Oliveira, Lucianne Ferreira Paes Rojas-Villalta, Dorian de Lira, Simone Possedente Nunez-Montero, Kattia
Título	Metabologenomics analysis of Pseudomonas sp. So3.2b, an Antarctic strain with bioactivity against Rhizoctonia solani
Editorial	FRONTIERS MEDIA SA
Revista	FRONTIERS IN MICROBIOLOGY
Lenguaje	en
Resumen	IntroductionPhytopathogenic fungi are a considerable concern for agriculture, as they can threaten the productivity of several crops worldwide. Meanwhile, natural microbial products are acknowledged to play an important role in modern agriculture as they comprehend a safer alternative to synthetic pesticides. Bacterial strains from underexplored environments are a promising source of bioactive metabolites. MethodsWe applied the OSMAC (One Strain, Many Compounds) cultivation approach, in vitro bioassays, and metabolo-genomics analyses to investigate the biochemical potential of Pseudomonas sp. So3.2b, a strain isolated from Antarctica. Crude extracts from OSMAC were analyzed through HPLC-QTOF-MS/MS, molecular networking, and annotation. The antifungal potential of the extracts was confirmed against Rhizoctonia solani strains. Moreover, the whole-genome sequence was studied for biosynthetic gene clusters (BGCs) identification and phylogenetic comparison. Results and DiscussionMolecular networking revealed that metabolite synthesis has growth media specificity, and it was reflected in bioassays results against R. solani. Bananamides, rhamnolipids, and butenolides-like molecules were annotated from the metabolome, and chemical novelty was also suggested by several unidentified compounds. Additionally, genome mining confirmed a wide variety of BGCs present in this strain, with low to no similarity with known molecules. An NRPS-encoding BGC was identified as responsible for producing the banamides-like molecules, while phylogenetic analysis demonstrated a close relationship with other rhizosphere bacteria. Therefore, by combining -omics approaches and in vitro bioassays, our study demonstrates that Pseudomonas sp. So3.2b has potential application to agriculture as a source of bioactive metabolites.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3389/fmicb.2023.1187321
Formato Recurso	PDF
Palabras Claves	bioactivity OSMAC molecular networking secondary metabolites genomics biosynthetic gene cluster bioprospecting
Ubicación del archivo	http://dx.doi.org/10.3389/fmicb.2023.1187321
Categoría OCDE	Microbiología
Materias	bioactividad OSMAC redes moleculares metabolitos secundarios genómica grupo de genes biosintéticos bioprospección
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:000989926600001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 1210563 ANID-FONDECYT 11230475 INACH DG_01-19 NEXER NXR17-0003 TEC 5402-1510-1035 CONICYT-PFCHA 2017-21170263 CAPES 001 FAPESP 2019/17721-9 FAPESP 2022/01529-4 CNPQ 142260/2020-4 CNPEQ141501/2020-0 ANID FONDECYT 1210563 ANID FONDECYT 11230475 Instituto Tecnológico de Costa Rica 5402-1510-1035 ANID CONICYT-PFCHA 2017-21170263 CNPq 142260/2020-4 CNPq 141501/2020-0