Small extracellular vesicles from pregnant women with maternal supraphysiological hypercholesterolemia impair endothelial cell function in vitro

Primer Autor
Gutierrez, Jaime
Co-autores
Contreras-Duarte, Susana
Escalona-Rivano, Rodrigo
Cantin, Claudette
Valdivia, Pascuala
Zapata, David
Carvajal, Lorena
Brito, Roberto
Cerda, Alvaro
Illanes, Sebastian
Leiva, Andrea
Título
Small extracellular vesicles from pregnant women with maternal supraphysiological hypercholesterolemia impair endothelial cell function in vitro
Editorial
ELSEVIER SCIENCE INC
Revista
VASCULAR PHARMACOLOGY
Lenguaje
en
Resumen
Maternal physiological hypercholesterolemia MPH, maternal total cholesterol (TC) levels at term of pregnancy <280 mg/dL) occurs to assure fetal development. Maternal supraphysiological hypercholesterolemia (MSPH, TC levels >280 mg/dL) is a pathological condition associated with maternal, placental, and fetal endothelial dysfunction and early neonatal atherosclerosis development. Small extracellular vesicles (sEVs) are delivered to the extracellular space by different cells, where they modulate cell functions by transporting active signaling molecules, including proteins and miRNA.Aim: To determine whether sEVs from MSPH women could alter the function of endothelial cells (angiogenesis, endothelial activation and nitric oxide synthesis capacity).Methods: This study included 24 Chilean women (12 MPH and 12 MSPH). sEVs were isolated from maternal plasma and characterized by sEV markers (CD9, Alix and HSP70), nanoparticle tracking analysis, transmission electron microscopy, and protein and cholesterol content. The endothelial cell line HMEC-1 was used to determine the uptake of labeled sEVs and the effects of sEVs on cell viability, endothelial tube formation, endothelial cell activation, and endothelial nitric oxide expression and function.Results: In MSPH women, the plasma concentration of sEVs was increased compared to that in MPH women. MSPH-sEVs were highly taken up by HMEC-1 cells and reduced angiogenic capacity and the expression and activity of eNOS without changing cell viability or endothelial activation compared to MPH-sEVs. Conclusion: sEVs from MSPH women impair angiogenesis and nitric oxide synthesis in endothelial cells, which could contribute to MSPH-associated endothelial dysfunction.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.1016/j.vph.2023.107174
Formato Recurso
PDF
Palabras Claves
Cholesterol
Pregnancy
Small extracellular vesicles
Endothelial function
Angiogenesis
Nitric oxide
Ubicación del archivo
http://dx.doi.org/10.1016/j.vph.2023.107174
Categoría OCDE
Farmacología y farmacia
Materias
Colesterol
embarazo
Pequeñas vesículas extracelulares
función endotelial
Angiogénesis
Óxido nítrico
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
Derechos de acceso
metadata
Access Rights
metadata
Id de Web of Science
WOS:001001231600001
ISSN
1537-1891
Tipo de ruta
hibrida
Categoría WOS
Farmacología y farmacia
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 1221362
ANID-FONDECYT 1190250
ANID-FONDECYT 1230527
ANID FB210024
ANID FONDECYT 1221362
ANID FONDECYT 1190250
ANID FONDECYT 1230527
Colecciones
Colección Publicaciones Científicas
Colección ANID
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