Repositorio UFRO · Omeka S

Unique genomic sequences in a novel Mycobacterium avium subsp. hominissuis lineage enable fine scale transmission route tracing during pig movement

Primer Autor	Maruyama, Fumito
Co-autores	Komatsu, Tetsuya Ohya, Kenji Ota, Atsushi Nishiuchi, Yukiko Yano, Hirokazu Matsuo, Kayoko Odoi, Justice Opare Suganuma, Shota Sawai, Kotaro Hasebe, Akemi Asai, Tetsuo Yanai, Tokuma Fukushi, Hideto Wada, Takayuki Yoshida, Shiomi Ito, Toshihiro Arikawa, Kentaro Kawai, Mikihiko Ato, Manabu Baughn, Anthony D. Iwamoto, Tomotada
Título	Unique genomic sequences in a novel Mycobacterium avium subsp. hominissuis lineage enable fine scale transmission route tracing during pig movement
Editorial	ELSEVIER
Revista	ONE HEALTH
Lenguaje	en
Resumen	Mycobacterium avium subsp. hominissuis (MAH) is one of the most prevalent mycobacteria causing nontuberculous mycobacterial disease in humans and animals. Of note, MAH is a major cause of mycobacterial granulomatous mesenteric lymphadenitis outbreaks in pig populations. To determine the precise source of infection of MAH in a pig farm and to clarify the epidemiological relationship among pig, human and environmental MAH lineages, we collected 50 MAH isolates from pigs reared in Japan and determined draft genome sequences of 30 isolates. A variable number of tandem repeat analysis revealed that most pig MAH isolates in Japan were closely related to North American, European and Russian human isolates but not to those from East Asian human and their residential environments. Historical recombination analysis revealed that most pig isolates could be classified into SC2/4 and SC3, which contain MAH isolated from pig, European human and environmental isolates. Half of the isolates in SC2/4 had many recombination events with MAH lineages isolated from humans in East Asia. To our surprise, four isolates belonged to a new lineage (SC5) in the global MAH population. Members of SC5 had few footprints of inter-lineage recombination in the genome, and carried 80 unique genes, most of which were located on lineage specific-genomic islands. Using unique genetic features, we were able to trace the putative transmission route via their host pigs. Together, we clarify the possibility of species-specificity of MAH in addition to local adaptation. Our results highlight two transmission routes of MAH, one exposure on pig farms from the environment and the other via pig movement. Moreover, our study also warns that the evolution of MAH in pigs is influenced by MAH from patients and their residential environments, even if the MAH are genetically distinct.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.onehlt.2023.100559
Formato Recurso	PDF
Palabras Claves	MAH VNTR Draft genome sequences Pig Transmission route
Ubicación del archivo	http://dx.doi.org/10.1016/j.onehlt.2023.100559
Categoría OCDE	Salud pública, ambiental y ocupacional Enfermedades infecciosas
Materias	MAH VNTR Proyectos de secuencias del genoma Cerdo Ruta de transmisión
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY-NC-ND 4.0
Condición de la licencia (Recomendado-repetible)	CC BY-NC-ND 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001045473200001
Tipo de ruta	Verde# dorado
Categoría WOS	Salud pública, ambiental y ocupacional Enfermedades infecciosas
Referencia del Financiador (Mandatado si es aplicable-repetible)	AMED 20wm0225012h0001, 23fk0108673h0401 JRA H28-29_239, H29-30_7 JRA H28-130, H30-60 JSPS JP26304039 JSPS 18KK0436 JSPS 20H00562 JSPS 20H00451