Repositorio
Institucional

Jorquera, Milko A.

Acuna, Jacquelinne J.

Rilling, Joaquin I.

Inostroza, Nitza G.

Manquian, Javiera

Zhang, Qian

Gupta, Vadakattu V. S. R.

Diversity, Community Structure, and Potential Functions of Root-Associated Bacterial Communities of Different Wheat (Triticum aestivum) Cultivars under Field Conditions

MDPI

AGRONOMY-BASEL

en

The wheat (Triticum aestivum) microbiome is essential to its growth and adaptation under the current climatic crisis. Wheat breeding programs are often mainly focused on obtaining more resistant cultivars, thus, plant genotype-by-microbiome interactions have gained attention. In this sense, local wheat cultivars represent a unique opportunity to examine how bacterial communities are recruited and support plant growth under field conditions. In this study, we explored the diversity, community structure, and potential functions of root-associated bacterial communities of four Chilean wheat (Triticum aestivum) cultivars under field conditions through Illumina MiSeq. Analyses showed that Proteobacteria was the most abundant phylum in root endosphere (51.1 to 74.4%) and rhizosphere samples (39.3 to 44.9%) across wheat cultivars. Significant differences (p = 0.05) in alpha and beta diversity were observed in root endosphere and rhizosphere samples, independently of wheat genotypes. Potassium was identified as the main factor driving the rhizosphere microbiomes of wheat. A higher proportion of shared operational taxonomic units (OTUs) were found in rhizosphere (mainly Pseudomonas, Flavobacterium, and Janthinobacterium) compared with root endosphere (dominated by Delftia, Acinetobacter, Stenotrophomonas, Kaistobacter) samples across all cultivars. Analyses of larger predicted functional activities revealed that chemoheterotrophy and aerobic chemoheterotrophy were more observed in the root endosphere environment, whereas among the minor functions, nitrogen cycling was the more predicted trait, related to rhizosphere samples. A co-occurrence analysis revealed complex bacterial interactions in wheat cultivars' niche microbiomes identifying three (Comamonadaceae, Enterobacteraceae, Micrococcaceae) and four (Corynebacteraceae, Dermabacteraceae, Xanthomonadaceae, Staphylococcaceae) families as keystone taxa for the root endosphere and rhizosphere, respectively. It is suggested that such findings on the differences in root microbiomes associated with wheat cultivars under field conditions would help to develop new cultivars with abilities to recruit specific bacterial communities.

2023

artículo original

10.3390/agronomy13051392

PDF

root microbiome

wheat cultivars

bacterial diversity

putative functions

Agricultura

Ciencias Vegetales

microbioma de la raíz

cultivares de trigo

diversidad bacteriana

funciones putativas

artículo original

versión publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

WOS:000994673500001

verde# dorado

Agricultura

Ciencias Vegetales

ANID-FONDECYT 1201386

ANID-FONDECYT 1221228

ANID-FONDECYT 3210594

DIUFRO DI21-0044

ICN2021_044

ANID FONDECYT 1201386

ANID FONDECYT 1221228

ANID FONDECYT 3210594

UFRO DI21-0044