Repositorio UFRO · Omeka S

FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa

Primer Autor	Sanchez, Raul
Co-autores	Uribe, Pamela Barra, Javiera Painen, Kevin Zambrano, Fabiola Schulz, Mabel Moya, Claudia Isachenko, Vladimir Isachenko, Evgenia Mallmann, Peter
Título	FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa
Editorial	MDPI
Revista	ANTIOXIDANTS
Lenguaje	en
Resumen	Excessive levels of reactive nitrogen species (RNS), such as peroxynitrite, promote nitrosative stress, which is an important cause of impaired sperm function. The metalloporphyrin FeTPPS is highly effective in catalyzing the decomposition of peroxynitrite, reducing its toxic effects in vivo and in vitro. FeTPPS has significant therapeutic potential in peroxynitrite-related diseases, however, its effects on human spermatozoa under nitrosative stress have not been described. This work aimed to evaluate the in vitro effect of FeTPPS against peroxynitrite-mediated nitrosative stress in human spermatozoa. For this purpose, spermatozoa from normozoospermic donors were exposed to 3-morpholinosydnonimine, a molecule that generates peroxynitrite. First, the FeTPPS-mediated peroxynitrite decomposition catalysis was analyzed. Then, its individual effect on sperm quality parameters was evaluated. Finally, the effect of FeTPPS on ATP levels, motility, mitochondrial membrane potential, thiol oxidation, viability, and DNA fragmentation was analyzed in spermatozoa under nitrosative stress conditions. The results showed that FeTPPS effectively catalyzes the decomposition of peroxynitrite without affecting sperm viability at concentrations up to 50 & mu,mol/L. Furthermore, FeTPPS mitigates the deleterious effects of nitrosative stress on all sperm parameters analyzed. These results highlight the therapeutic potential of FeTPPS in reducing the negative impact of nitrosative stress in semen samples with high RNS levels.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/antiox12061272
Formato Recurso	PDF
Palabras Claves	nitrosative stress peroxynitrite human sperm antioxidant
Ubicación del archivo	http://dx.doi.org/10.3390/antiox12061272
Categoría OCDE	Bioquímica y biología molecular Farmacología y farmacia Ciencia y tecnología de los alimentos
Materias	estrés nitrosativo peroxinitrito esperma humano antioxidante
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001016838300001
Tipo de ruta	verde# dorado
Categoría WOS	Bioquímica y biología molecular Farmacología y farmacia Ciencia y tecnología de los alimentos
Referencia del Financiador (Mandatado si es aplicable-repetible)	UFRO DI22-3034 UFRO DIE17-0012