FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa
| Primer Autor |
Sanchez, Raul
|
| Co-autores |
Uribe, Pamela
Barra, Javiera
Painen, Kevin
Zambrano, Fabiola
Schulz, Mabel
Moya, Claudia
Isachenko, Vladimir
Isachenko, Evgenia
Mallmann, Peter
|
| Título |
FeTPPS, a Peroxynitrite Decomposition Catalyst, Ameliorates Nitrosative Stress in Human Spermatozoa
|
| Editorial |
MDPI
|
| Revista |
ANTIOXIDANTS
|
| Lenguaje |
en
|
| Resumen |
Excessive levels of reactive nitrogen species (RNS), such as peroxynitrite, promote nitrosative stress, which is an important cause of impaired sperm function. The metalloporphyrin FeTPPS is highly effective in catalyzing the decomposition of peroxynitrite, reducing its toxic effects in vivo and in vitro. FeTPPS has significant therapeutic potential in peroxynitrite-related diseases, however, its effects on human spermatozoa under nitrosative stress have not been described. This work aimed to evaluate the in vitro effect of FeTPPS against peroxynitrite-mediated nitrosative stress in human spermatozoa. For this purpose, spermatozoa from normozoospermic donors were exposed to 3-morpholinosydnonimine, a molecule that generates peroxynitrite. First, the FeTPPS-mediated peroxynitrite decomposition catalysis was analyzed. Then, its individual effect on sperm quality parameters was evaluated. Finally, the effect of FeTPPS on ATP levels, motility, mitochondrial membrane potential, thiol oxidation, viability, and DNA fragmentation was analyzed in spermatozoa under nitrosative stress conditions. The results showed that FeTPPS effectively catalyzes the decomposition of peroxynitrite without affecting sperm viability at concentrations up to 50 & mu,mol/L. Furthermore, FeTPPS mitigates the deleterious effects of nitrosative stress on all sperm parameters analyzed. These results highlight the therapeutic potential of FeTPPS in reducing the negative impact of nitrosative stress in semen samples with high RNS levels.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.3390/antiox12061272
|
| Formato Recurso |
PDF
|
| Palabras Claves |
nitrosative stress
peroxynitrite
human sperm
antioxidant
|
| Ubicación del archivo | |
| Categoría OCDE |
Bioquímica y biología molecular
Farmacología y farmacia
Ciencia y tecnología de los alimentos
|
| Materias |
estrés nitrosativo
peroxinitrito
esperma humano
antioxidante
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001016838300001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Bioquímica y biología molecular
Farmacología y farmacia
Ciencia y tecnología de los alimentos
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
UFRO DI22-3034
UFRO DIE17-0012
|
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