Immunofluorescence Analysis of Estrogen and Progesterone Receptors and Ki-67 Nuclear Protein in Canine Uteri Treated with Medroxyprogesterone Acetate During Anestrus
| Primer Autor |
Salinas, Paulo
|
| Co-autores |
Miglino, Maria Angelica
del Sol, Mariano
|
| Título |
Immunofluorescence Analysis of Estrogen and Progesterone Receptors and Ki-67 Nuclear Protein in Canine Uteri Treated with Medroxyprogesterone Acetate During Anestrus
|
| Editorial |
SOC CHILENA ANATOMIA
|
| Revista |
INTERNATIONAL JOURNAL OF MORPHOLOGY
|
| Lenguaje |
en
|
| Resumen |
Estradiol and progesterone receptors play an essential role in the changes occurring in the uterus during the estrus cycle in dogs (Canis lupus familiaris). In order to investigate the potential effect of progestational agent medroxyprogesterone acetate (MPA) when is used during anestrus on the expression of estradiol receptors [ER], progesterone receptors [PR] and nuclear protein Ki67, we evaluated uterine tissue immunohistochemically. Uteri were grouped as nulliparous (control, n=11), multiparous (n=11) and treated with MPA (n=11, nulliparous with two treatments, 5mg/kg, i.m.). The amount and location of PR, ER and Ki67 were studied on the epithelial surface, apical and basal regions of the endometrium and myometrium using immunohistochemical techniques with a spectral confocal microscope and analyzed by ANOVA. Differences in ER were observed between the multiparous and MPA-treated groups in the apical region of the endometrium (p=0.0022). Differences in cell proliferation were detected between the nulliparous and multiparous groups (p=0.0037) and nulliparous and MPA-treated groups (p=0.0003) in the basal region of the endometrium. In conclusion, two doses of MPA (5mg/kg, i.m.) do not have a significant effect on the expression of ER and PR, however, they inhibit cell proliferation in the basal region of the endometrium, which includes the stroma, subepithelial cell layer, compact layer, and spongy layer. The clinical and long-term effect of this treatment should be evaluated in subsequent studies.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.4067/S0717-95022023000300725
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Canis lupus familiaris
Uterus
Medroxyprogesterone acetate
Estradiol
Progesterone
Ki67
|
| Ubicación del archivo | |
| Categoría OCDE |
Anatomía y Morfología
|
| Materias |
Canis lupus familiaris
Útero
acetato de medroxiprogesterona
estradiol
Progesterona
Ki67
|
| Página de inicio (Recomendado-único) |
725.0
|
| Página final (Recomendado-único) |
732
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001033597900007
|
| ISSN |
0717-9502
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Anatomía y Morfología
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
CONICYT D 21140825
ANID CONICYT D 21140825
|
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