Repositorio UFRO · Omeka S

Immunofluorescence Analysis of Estrogen and Progesterone Receptors and Ki-67 Nuclear Protein in Canine Uteri Treated with Medroxyprogesterone Acetate During Anestrus

Primer Autor	Salinas, Paulo
Co-autores	Miglino, Maria Angelica del Sol, Mariano
Título	Immunofluorescence Analysis of Estrogen and Progesterone Receptors and Ki-67 Nuclear Protein in Canine Uteri Treated with Medroxyprogesterone Acetate During Anestrus
Editorial	SOC CHILENA ANATOMIA
Revista	INTERNATIONAL JOURNAL OF MORPHOLOGY
Lenguaje	en
Resumen	Estradiol and progesterone receptors play an essential role in the changes occurring in the uterus during the estrus cycle in dogs (Canis lupus familiaris). In order to investigate the potential effect of progestational agent medroxyprogesterone acetate (MPA) when is used during anestrus on the expression of estradiol receptors [ER], progesterone receptors [PR] and nuclear protein Ki67, we evaluated uterine tissue immunohistochemically. Uteri were grouped as nulliparous (control, n=11), multiparous (n=11) and treated with MPA (n=11, nulliparous with two treatments, 5mg/kg, i.m.). The amount and location of PR, ER and Ki67 were studied on the epithelial surface, apical and basal regions of the endometrium and myometrium using immunohistochemical techniques with a spectral confocal microscope and analyzed by ANOVA. Differences in ER were observed between the multiparous and MPA-treated groups in the apical region of the endometrium (p=0.0022). Differences in cell proliferation were detected between the nulliparous and multiparous groups (p=0.0037) and nulliparous and MPA-treated groups (p=0.0003) in the basal region of the endometrium. In conclusion, two doses of MPA (5mg/kg, i.m.) do not have a significant effect on the expression of ER and PR, however, they inhibit cell proliferation in the basal region of the endometrium, which includes the stroma, subepithelial cell layer, compact layer, and spongy layer. The clinical and long-term effect of this treatment should be evaluated in subsequent studies.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.4067/S0717-95022023000300725
Formato Recurso	PDF
Palabras Claves	Canis lupus familiaris Uterus Medroxyprogesterone acetate Estradiol Progesterone Ki67
Ubicación del archivo	http://dx.doi.org/10.4067/S0717-95022023000300725
Categoría OCDE	Anatomía y Morfología
Materias	Canis lupus familiaris Útero acetato de medroxiprogesterona estradiol Progesterona Ki67
Página de inicio (Recomendado-único)	725.0
Página final (Recomendado-único)	732
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001033597900007
ISSN	0717-9502
Tipo de ruta	verde# dorado
Categoría WOS	Anatomía y Morfología
Referencia del Financiador (Mandatado si es aplicable-repetible)	CONICYT D 21140825 ANID CONICYT D 21140825