Repositorio UFRO · Omeka S

Bone marrow mesenchymal/fibroblastic stromal cells induce a distinctive EMT-like phenotype in AML cells

Primer Autor	Calle, Yolanda
Co-autores	Nojszewska, N. Idilli, O. Sarkar, D. Ahouiyek, Z. Arroyo-Berdugo, Y. Sandoval, C. Amin-Anjum, M. S. Bowers, S. Greaves, D. Saeed, L. Khan, M. Salti, S. Al-Shami, S. Topoglu, H. Punzalan, J. K. Farias, J. G.
Título	Bone marrow mesenchymal/fibroblastic stromal cells induce a distinctive EMT-like phenotype in AML cells
Editorial	ELSEVIER GMBH
Revista	EUROPEAN JOURNAL OF CELL BIOLOGY
Lenguaje	en
Resumen	The development of epithelial-to-mesenchymal transition (EMT) like features is emerging as a critical factor involved in the pathogenesis of acute myeloid leukaemia (AML). However, the extracellular signals and the signalling pathways in AML that may regulate EMT remain largely unstudied. We found that the bone marrow (BM) mesenchymal/fibroblastic cell line HS5 induces an EMT-like migratory phenotype in AML cells. AML cells underwent a strong increase of vimentin (VIM) levels that was not mirrored to the same extent by changes of expression of the other EMT core proteins SNAI1 and SNAI2. We validated these particular pattern of coexpression of core-EMT markers in AML cells by performing an in silico analysis using datasets of human tumours. Our data showed that in AML the expression levels of VIM does not completely correlate with the coexpression of core EMT markers observed in epithelial tumours. We also found that vs epithelial tumours, AML cells display a distinct patterns of co-expression of VIM and the actin binding and adhesion regulatory proteins that regulate F-actin dynamics and integrin-mediated adhesions involved in the invasive migration in cells undergoing EMT. We conclude that the BM stroma induces an EMT related pattern of migration in AML cells in a process involving a distinctive regulation of EMT markers and of regulators of cell adhesion and actin dynamics that should be further investigated. Understanding the tumour specific signalling pathways associated with the EMT process may contribute to the development of new tailored therapies for AML as well as in different types of cancers.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.ejcb.2023.151334
Formato Recurso	PDF
Palabras Claves	AML Tumour microenvironment EMT Migration Vimentin F-actin Cell adhesion
Ubicación del archivo	http://dx.doi.org/10.1016/j.ejcb.2023.151334
Categoría OCDE	Biología Celular
Materias	LMA Microambiente tumoral paramédico Migración vimentina actina F Adhesión celular
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY-NC-ND 4.0
Condición de la licencia (Recomendado-repetible)	CC BY-NC-ND 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001024777200001
ISSN	0171-9335
Tipo de ruta	Verde# dorado
Categoría WOS	Biología Celular
Referencia del Financiador (Mandatado si es aplicable-repetible)	CRUK C34579/A20784