Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems
| Primer Autor |
Zamorano, Mauricio
|
| Co-autores |
Lefin, Nicolas
Miranda, Javiera
Beltran, Jorge F.
Belen, Lisandra Herrera
Effer, Brian
Pessoa Jr, Adalberto
Farias, Jorge G.
|
| Título |
Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems
|
| Editorial |
FRONTIERS MEDIA SA
|
| Revista |
FRONTIERS IN PHARMACOLOGY
|
| Lenguaje |
en
|
| Resumen |
Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to optimize the expression of L-ASNase in heterologous systems. This article describes various approaches that have been employed to increase enzyme production, including the use of molecular tools, strain engineering, and in silico optimization. The review article highlights the critical role that rational design plays in achieving successful heterologous expression and underscores the challenges of large-scale production of L-ASNase, such as inadequate protein folding and the metabolic burden on host cells. Improved gene expression is shown to be achievable through the optimization of codon usage, synthetic promoters, transcription and translation regulation, and host strain improvement, among others. Additionally, this review provides a deep understanding of the enzymatic properties of L-ASNase and how this knowledge has been employed to enhance its properties and production. Finally, future trends in L-ASNase production, including the integration of CRISPR and machine learning tools are discussed. This work serves as a valuable resource for researchers looking to design effective heterologous expression systems for L-ASNase production as well as for enzymes production in general.
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| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo de revisión
|
| doi |
10.3389/fphar.2023.1208277
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| Formato Recurso |
PDF
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| Palabras Claves |
L-asparaginase
molecular strategies
rational design
heterologous expression system
industrial bioprocessing
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| Ubicación del archivo | |
| Categoría OCDE |
Farmacología y farmacia
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| Materias |
L-asparaginasa
estrategias moleculares
diseño racional
sistema de expresión heteróloga
bioprocesamiento industrial
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| Identificador del recurso (Mandatado-único) |
artículo de revisión
|
| Versión del recurso (Recomendado-único) |
versión publicada
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| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001023522000001
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| Tipo de ruta |
verde# dorado
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| Categoría WOS |
Farmacología y farmacia
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
FAPESP UFRO 2020/06982-3
ANID-FONDECYT 3210142
ANID FONDECYT 3210142
|
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