Repositorio UFRO · Omeka S

Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems

Primer Autor	Zamorano, Mauricio
Co-autores	Lefin, Nicolas Miranda, Javiera Beltran, Jorge F. Belen, Lisandra Herrera Effer, Brian Pessoa Jr, Adalberto Farias, Jorge G.
Título	Current state of molecular and metabolic strategies for the improvement of L-asparaginase expression in heterologous systems
Editorial	FRONTIERS MEDIA SA
Revista	FRONTIERS IN PHARMACOLOGY
Lenguaje	en
Resumen	Heterologous expression of L-asparaginase (L-ASNase) has become an important area of research due to its clinical and food industry applications. This review provides a comprehensive overview of the molecular and metabolic strategies that can be used to optimize the expression of L-ASNase in heterologous systems. This article describes various approaches that have been employed to increase enzyme production, including the use of molecular tools, strain engineering, and in silico optimization. The review article highlights the critical role that rational design plays in achieving successful heterologous expression and underscores the challenges of large-scale production of L-ASNase, such as inadequate protein folding and the metabolic burden on host cells. Improved gene expression is shown to be achievable through the optimization of codon usage, synthetic promoters, transcription and translation regulation, and host strain improvement, among others. Additionally, this review provides a deep understanding of the enzymatic properties of L-ASNase and how this knowledge has been employed to enhance its properties and production. Finally, future trends in L-ASNase production, including the integration of CRISPR and machine learning tools are discussed. This work serves as a valuable resource for researchers looking to design effective heterologous expression systems for L-ASNase production as well as for enzymes production in general.
Fecha Publicación	2023
Tipo de Recurso	artículo de revisión
doi	10.3389/fphar.2023.1208277
Formato Recurso	PDF
Palabras Claves	L-asparaginase molecular strategies rational design heterologous expression system industrial bioprocessing
Ubicación del archivo	http://dx.doi.org/10.3389/fphar.2023.1208277
Categoría OCDE	Farmacología y farmacia
Materias	L-asparaginasa estrategias moleculares diseño racional sistema de expresión heteróloga bioprocesamiento industrial
Identificador del recurso (Mandatado-único)	artículo de revisión
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001023522000001
Tipo de ruta	verde# dorado
Categoría WOS	Farmacología y farmacia
Referencia del Financiador (Mandatado si es aplicable-repetible)	FAPESP UFRO 2020/06982-3 ANID-FONDECYT 3210142 ANID FONDECYT 3210142