Repositorio
Institucional

Ili, Carmen Gloria

Reyes, Maria Elena

Zanella, Louise

Riquelme, Ismael

Buchegger, Kurt

Mora-Lagos, Barbara

Guzman, Pablo

Garcia, Patricia

Roa, Juan C.

Brebi, Priscilla

Exploring the Genetic Diversity of Epstein-Barr Virus among Patients with Gastric Cancer in Southern Chile

MDPI

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

en

The Epstein-Barr virus (EBV) has been associated with gastric cancer (GC), one of the deadliest malignancies in Chile and the world. Little is known about Chilean EBV strains. This study aims to investigate the frequency and genetic diversity of EBV in GC in patients in southern Chile. To evaluate the prevalence of EBV in GC patients from the Chilean population, we studied 54 GC samples using the gold standard detection method of EBV-encoded small RNA (EBER). The EBV-positive samples were subjected to amplification and sequencing of the Epstein-Barr virus nuclear protein 3A (EBNA3A) gene to evaluate the genetic diversity of EBV strains circulating in southern Chile. In total, 22.2% of the GC samples were EBV-positive and significantly associated with diffuse-type histology (p = 0.003). Phylogenetic analyses identified EBV-1 and EBV-2 in the GC samples, showing genetic diversity among Chilean isolates. This work provides important information for an epidemiological follow-up of the different EBV subtypes that may cause GC in southern Chile.

2023

artículo original

10.3390/ijms241411276

PDF

gastric cancer

Epstein-Barr virus (EBV)

EBNA3A

phylogeny

Chile

Bioquímica y biología molecular

Química

cáncer gástrico

virus de Epstein-Barr (VEB)

EBNA3A

filogenia

Chile

artículo original

versión publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

WOS:001035902500001

1661-6596

verde# dorado

Bioquímica y biología molecular

Química