Repositorio UFRO · Omeka S

Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica

Primer Autor	Bravo, Leon A.
Co-autores	Lopez, Dariel Larama, Giovanni Saez, Patricia L.
Título	Transcriptome Analysis of Diurnal and Nocturnal-Warmed Plants, the Molecular Mechanism Underlying Cold Deacclimation Response in Deschampsia antarctica
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje	en
Resumen	Warming in the Antarctic Peninsula is one of the fastest on earth, and is predicted to become more asymmetric in the near future. Warming has already favored the growth and reproduction of Antarctic plant species, leading to a decrease in their freezing tolerance (deacclimation). Evidence regarding the effects of diurnal and nocturnal warming on freezing tolerance-related gene expression in D. antarctica is negligible. We hypothesized that freezing tolerance-related gene (such as CBF-regulon) expression is reduced mainly by nocturnal warming rather than diurnal temperature changes in D. antarctica. The present work aimed to determine the effects of diurnal and nocturnal warming on cold deacclimation and its associated gene expression in D. antarctica, under laboratory conditions. Fully cold-acclimated plants (8 degrees C/0 degrees C), with 16h/8h thermoperiod and photoperiod duration, were assigned to four treatments for 14 days: one control (8 degrees C/0 degrees C) and three with different warming conditions (diurnal (14 degrees C/0 degrees C), nocturnal (8 degrees C/6 degrees C), and diurnal-nocturnal (14 degrees C/6 degrees C). RNA-seq was performed and differential gene expression was analyzed. Nocturnal warming significantly down-regulated the CBF transcription factors expression and associated cold stress response genes and up-regulated photosynthetic and growth promotion genes. Consequently, nocturnal warming has a greater effect than diurnal warming on the cold deacclimation process in D. antarctica. The eco-physiological implications are discussed.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/ijms241311211
Formato Recurso	PDF
Palabras Claves	Antarctic plant asymmetric warming CBF climate change cold deacclimation gene expression RNA-seq
Ubicación del archivo	http://dx.doi.org/10.3390/ijms241311211
Categoría OCDE	Bioquímica y biología molecular Química
Materias	Planta antártica calentamiento asimétrico FBC cambio climático desaclimatación fría la expresion genica secuencia de ARN
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001030891300001
ISSN	1661-6596
Tipo de ruta	verde# dorado
Categoría WOS	Bioquímica y biología molecular Química
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 1151173 ANID 21170470 UFRO NEXER NXR17-0002 INACH RT 18-18 ANID FONDECYT 1151173