Behavioral Study of 3-and 5-Halocytisine Derivatives in Zebrafish Using the Novel Tank Diving Test (NTT)

Primer Autor
Iturriaga-Vasquez, Patricio
Co-autores
Farias-Cea, Amaury
Leal, Cristobal
Hoedar-Salazar, Martin
Esparza, Erica
Martinez-Duran, Luis
Fuentes, Irma
Título
Behavioral Study of 3-and 5-Halocytisine Derivatives in Zebrafish Using the Novel Tank Diving Test (NTT)
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
Anxiety is a serious mental disorder, and recent statistics have determined that 35.12% of the global population had an anxiety disorder during the COVID-19 pandemic. A mechanism associated with anxiolytic effects is related to nicotinic acetylcholine receptor (nAChR) agonists, principally acting on the & alpha,4 & beta,2 nAChR subtype. nAChRs are present in different animal models, including murine and teleosteos ones. Zebrafish has become an ideal animal model due to its high human genetic similarities (70%), giving it high versatility in different areas of study, among them in behavioral studies related to anxiety. The novel tank diving test (NTT) is one of the many paradigms used for studies on new drugs related to their anxiolytic effect. In this work, an adult zebrafish was used to determine the behavioral effects of 3- and 5-halocytisine derivatives, using the NTT at different doses. Our results show that substitution at position 3 by chlorine or bromine decreases the time spent by the fish at the bottom compared to the control. However, the 3-chloro derivative at higher doses increases the bottom dwelling time. In contrast, substitution at the 5 position increases bottom dwelling at all concentrations showing no anxiolytic effects in this model. Unexpected results were observed with the 5-chlorocytisine derivative, which at a concentration of 10 mg/L produced a significant decrease in bottom dwelling and showed high times of freezing. In conclusion, the 3-chloro and 3-bromo derivatives show an anxiolytic effect, the 3-chlorocytisine derivative being more potent than the 3-bromo derivative, with the lowest time at the bottom of the tank at 1mg/L. On the other hand, chlorine, and bromine at position 5 produce an opposite effect.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/ijms241310635
Formato Recurso
PDF
Palabras Claves
novel tank diving test (NTT)
anxiety
halogenated cytisine
zebrafish
nicotinic acetylcholine receptors (nAChRs)
Ubicación del archivo
Categoría OCDE
Bioquímica y biología molecular
Química
Materias
nueva prueba de buceo con tanque (NTT)
ansiedad
citisina halogenada
pez cebra
Receptores nicotínicos de acetilcolina (nAChR)
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:001031121800001
ISSN
1661-6596
Tipo de ruta
verde# dorado
Categoría WOS
Bioquímica y biología molecular
Química
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID 21200389
ANID 21210358
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