Repositorio UFRO · Omeka S

Cathepsin B Is Not an Intrinsic Factor Related to Asparaginase Resistance of the Acute Lymphoblastic Leukemia REH Cell Line

Primer Autor	Monteiro, Gisele
Co-autores	Costa, Iris Munhoz Effer, Brian Costa-Silva, Tales Alexandre Chen, Chen Ciccone, Michael F. Pessoa, Adalberto dos Santos, Camila O.
Título	Cathepsin B Is Not an Intrinsic Factor Related to Asparaginase Resistance of the Acute Lymphoblastic Leukemia REH Cell Line
Editorial	MDPI
Revista	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje	en
Resumen	L-Asparaginase (ASNase) is a biopharmaceutical used as an essential drug in the treatment of acute lymphoblastic leukemia (ALL). Yet, some cases of ALL are naturally resistant to ASNase treatment, which results in poor prognosis. The REH ALL cell line, used as a model for studying the most common subtype of ALL, is considered resistant to treatment with ASNase. Cathepsin B (CTSB) is one of the proteases involved in the regulation of in vivo ASNase serum half-life and it has also been associated with the progression and resistance to treatment of several solid tumors. Previous works have shown that, in vitro, ASNase is degraded when incubated with REH cell lysate, which is prevented by a specific CTSB inhibitor, suggesting a function of this protease in the ASNase resistance of REH cells. In this work, we utilized a combination of CRISPR/Cas9 gene targeting and enzymatic measurements to investigate the relevance of CTSB on ASNase treatment resistance in the ALL model cell line. We found that deletion of CTSB in REH ALL cells did not confer ASNase treatment sensitivity, thus suggesting that intrinsic expression of CTSB is not a mechanism that drives the resistant nature of these ALL cells to enzymes used as the first-line treatment against leukemia.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/ijms241311215
Formato Recurso	PDF
Palabras Claves	asparaginase cathepsin B acute lymphoblastic leukemia treatment resistance genetic editing
Ubicación del archivo	http://dx.doi.org/10.3390/ijms241311215
Categoría OCDE	Bioquímica y biología molecular Química
Materias	asparaginasa catepsina B leucemia linfoblástica aguda resistencia al tratamiento edición genética
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001030130400001
ISSN	1661-6596
Tipo de ruta	verde# dorado
Categoría WOS	Bioquímica y biología molecular Química