Repositorio UFRO · Omeka S

MALDI-TOF MS: A Quick Method to Detect the Susceptibility of Fusarium spp. Clinical Isolates to Amphotericin B

Primer Autor	Santos, Cledir
Co-autores	Bariao, Patricia Helena Grizante Cayun, Yasna Sepulveda, Marcela Tonani, Ludmilla de Almeida, Otavio Guilherme Goncalves Cornejo, Pablo Dias, Nathalia Kress, Marcia Regina von Zeska
Título	MALDI-TOF MS: A Quick Method to Detect the Susceptibility of Fusarium spp. Clinical Isolates to Amphotericin B
Editorial	MDPI
Revista	MICROORGANISMS
Lenguaje	en
Resumen	Disseminated fusariosis is treated with amphotericin B and voriconazole. To determine adequate therapy, the minimal inhibitory concentration (MIC) is used. However, MIC analysis is based on visual observation and requires a long period of fungal incubation. The measure of the minimal profile change concentration (MPCC) using MALDI-TOF MS is a quick spectral method that has presented good results in determining the antimicrobial resistance of yeasts. However, there is a lack of information on filamentous fungi. In the present work, 13 Fusarium spp. clinical isolates and two reference strains were used. MIC was obtained according to the M38-A2 protocol of the Clinical Laboratory Standards Institute, while MPPC was obtained following the initial steps of the M38-A2 protocol. Both Biotyper and the Rstudio environment were used to analyze mass spectra. For some fungal strains, the data obtained from the software MALDI Biotyper Compass 4.1 led to fuzzy heatmaps resulting in difficult interpretation, while heatmaps obtained using Rstudio tools generated better MPCC resolutions. Herein, 86.6% of the AMB MPCC values were highly correlated with the gold-standard AMB MIC. MALDI-TOF MS is a prominent tool used to determine MPCCs quicker, cost-effectively, and more accurately for Fusarium spp. strains. However, better statistical analyses could help measure the technique's limit detection.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/microorganisms11071834
Formato Recurso	PDF
Palabras Claves	MPCC MIC fungal resistance
Ubicación del archivo	http://dx.doi.org/10.3390/microorganisms11071834
Categoría OCDE	Microbiología
Materias	MPCC micrófono resistencia a los hongos
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001038990000001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)	FAPESP UFRO 2020/07546-2 CAPES 001 CNPQ 310425/2021-2 MINEDUC FRO19101