MALDI-TOF MS: A Quick Method to Detect the Susceptibility of Fusarium spp. Clinical Isolates to Amphotericin B

Primer Autor
Santos, Cledir
Co-autores
Bariao, Patricia Helena Grizante
Cayun, Yasna
Sepulveda, Marcela
Tonani, Ludmilla
de Almeida, Otavio Guilherme Goncalves
Cornejo, Pablo
Dias, Nathalia
Kress, Marcia Regina von Zeska
Título
MALDI-TOF MS: A Quick Method to Detect the Susceptibility of Fusarium spp. Clinical Isolates to Amphotericin B
Editorial
MDPI
Revista
MICROORGANISMS
Lenguaje
en
Resumen
Disseminated fusariosis is treated with amphotericin B and voriconazole. To determine adequate therapy, the minimal inhibitory concentration (MIC) is used. However, MIC analysis is based on visual observation and requires a long period of fungal incubation. The measure of the minimal profile change concentration (MPCC) using MALDI-TOF MS is a quick spectral method that has presented good results in determining the antimicrobial resistance of yeasts. However, there is a lack of information on filamentous fungi. In the present work, 13 Fusarium spp. clinical isolates and two reference strains were used. MIC was obtained according to the M38-A2 protocol of the Clinical Laboratory Standards Institute, while MPPC was obtained following the initial steps of the M38-A2 protocol. Both Biotyper and the Rstudio environment were used to analyze mass spectra. For some fungal strains, the data obtained from the software MALDI Biotyper Compass 4.1 led to fuzzy heatmaps resulting in difficult interpretation, while heatmaps obtained using Rstudio tools generated better MPCC resolutions. Herein, 86.6% of the AMB MPCC values were highly correlated with the gold-standard AMB MIC. MALDI-TOF MS is a prominent tool used to determine MPCCs quicker, cost-effectively, and more accurately for Fusarium spp. strains. However, better statistical analyses could help measure the technique's limit detection.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/microorganisms11071834
Formato Recurso
PDF
Palabras Claves
MPCC
MIC
fungal resistance
Ubicación del archivo
http://dx.doi.org/10.3390/microorganisms11071834
Categoría OCDE
Microbiología
Materias
MPCC
micrófono
resistencia a los hongos
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:001038990000001
Tipo de ruta
verde# dorado
Categoría WOS
Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)
FAPESP UFRO 2020/07546-2
CAPES 001
CNPQ 310425/2021-2
MINEDUC FRO19101
Colecciones
Colección Publicaciones Científicas
Colección ANID
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