Repositorio
Institucional

Pumarola, Tomas

Pinana, Maria

Gonzalez-Sanchez, Alejandra

Andres, Cristina

Abanto, Michel

Vila, Jorgina

Esperalba, Juliana

Moral, Noelia

Espartosa, Elena

Saubi, Narcis

Creus, Anna

Codina, Maria Gema

Folgueira, Dolores

Martinez-Urtaza, Jaime

Anton, Andres

The emergence, impact, and evolution of human metapneumovirus variants from 2014 to 2021 in Spain

W B SAUNDERS CO LTD

JOURNAL OF INFECTION

en

Background: Human metapneumovirus (HMPV) is an important aetiologic agent of respiratory tract infection (RTI). This study aimed to describe the prevalence, genetic diversity, and evolutionary dynamics of HMPV. Methods: Laboratory-confirmed HMPV were characterised based on partial-coding G gene sequences with MEGA.v6.0. WGS was performed with Illumina, and evolutionary analyses with Datamonkey and Nextstrain. Results: HMPV prevalence was 2.5%, peaking in February-April and with an alternation in the predominance of HMPV-A and -B until the emergence of SARS-CoV-2, not circulating until summer and autumn-winter 2021, with a higher prevalence and with the almost only circulation of A2c111dup. G and SH proteins were the most variable, and 70% of F protein was under negative selection. Mutation rate of HMPV genome was 6.95 x 10-4 substitutions/site/year. Conclusion: HMPV showed a significant morbidity until the emergence of SARS-CoV-2 pandemic in 2020, not circulating again until summer and autumn 2021, with a higher prevalence and with almost the only circulation of A2c111dup, probably due to a more efficient immune evasion mechanism. The F protein showed a very conserved nature, supporting the need for steric shielding. The tMRCA showed a recent emergence of the A2c variants carrying duplications, supporting the importance of virological surveillance. & COPY, 2023 The Author(s). Published by Elsevier Ltd on behalf of The British Infection Association. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

2023

artículo original

10.1016/j.jinf.2023.05.004

PDF

Human metapneumovirus

Duplication

Epidemiology

Evolution

Whole-genome sequencing

Enfermedades infecciosas

metaneumovirus humano

Duplicación

Epidemiología

Evolución

Secuenciación del genoma completo

103.0

110

artículo original

versión publicada

CC BY-NC-ND 4.0

CC BY-NC-ND 4.0

acceso abierto

acceso abierto

WOS:001040222200001

0163-4453

Verde# hibrida

Enfermedades infecciosas

MINECO FISPI18/00685

REIPI RD16/0016/0003