Repositorio
Institucional

Revelo-Cueva, Maria

Toaquiza, Ana Belen

Gomez, Carlos

Ottone, Nicolas E.

Conservation of Organs (Heart, Brain and Kidney) of Canine by Cold-Temperature Silicone Plastination in an Animal Anatomy Laboratory in Ecuador

SOC CHILENA ANATOMIA

INTERNATIONAL JOURNAL OF MORPHOLOGY

en

For the purposes of teaching anatomy, the use of cadaver preparations is considered the most efficient way of ensuring that students retain knowledge. Nevertheless, in Ecuador the use of animal specimens in universities must comply with the internationally accepted principles of replacement, reduction and refinement (3Rs). Plastination is an alternative technique which allows organs to be conserved in the long term and complies with the 3Rs. The object of the present work was to use cold-temperature silicone plastination with Biodur & REG, products to obtain long-lasting, easy-to-handle canine organs for use as tools for the teaching of animal anatomy. Six canine cadavers were obtained from local animal protection charities. The hearts, brains and kidneys of the cadavers were dissected and fixed with formaldehyde 10 %. They were then dehydrated with acetone at -20 & DEG,C. The specimens were impregnated with Biodur & REG, S10:S3 (-20 & DEG,C) and finally cured with Biodur & REG, S6. We plastinated six hearts, twelve kidneys, four brains and one encephalic slice of canine. The application of cold-temperature plastination to canine organs followed the parameters established for the conventional protocol, enabling us to obtain organs of brilliant appearance, free of odours, in which the anatomical form was preserved. Thus the technique helped us to comply with the 3Rs, as we obtained easy-to-handle teaching models to replace fresh or formaldehyde-fixed samples for the teaching-learning of the canine anatomy.

2023

artículo original

10.4067/S0717-95022023000401004

PDF

Plastination

Cold -Temperature

3Rs

Teaching -learning

Anatomy

Anatomía y Morfología

Plastinación

Temperatura fría

3R

Enseñanza-aprendizaje

Anatomía

versión publicada

dorada

verde

acceso abierto

acceso abierto

WOS:001048344800003

1004.0

1008

artículo original