Repositorio UFRO · Omeka S

Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asuncion, Paraguay

Primer Autor	Arrua, Andrea
Co-autores	Moura-Mendes, Juliana Cazal-Martinez, Cinthia C. Rojas, Cinthia Ferreira, Francisco Perez-Estigarribia, Pastor Dias, Nathalia Godoy, Patricio Costa, Jessica Santos, Cledir
Título	Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asuncion, Paraguay
Editorial	MDPI
Revista	MICROORGANISMS
Lenguaje	en
Resumen	Zea mays var. amylacea and Zea mays var. indurata are maize ecotypes from Paraguay. Aspergillus section Flavi is the main spoilage fungus of maize under storage conditions. Due to its large intraspecific genetic variability, the accurate identification of this fungal taxonomic group is difficult. In the present study, potential mycotoxigenic strains of Aspergillus section Flavi isolated from Z. mays var. indurata and Z. mays var. amylacea that are marketed in the metropolitan region of Asuncion were identified by a polyphasic approach. Based on morphological characters, 211 isolates were confirmed to belong to Aspergillus section Flavi. A subset of 92 strains was identified as Aspergillus flavus by mass spectrometry MALDI-TOF and the strains were classified by MALDI-TOF MS into chemotypes based on their aflatoxins and cyclopiazonic acid production. According to the partial sequencing of ITS and CaM genes, a representative subset of 38 A. flavus strains was confirmed. Overall, 75 A. flavus strains (86%) were characterized as producers of aflatoxins. The co-occurrence of at least two mycotoxins (AF/ZEA, FUM/ZEA, and AF/ZEA/FUM) was detected for five of the Z. mays samples (63%). Considering the high mycological bioburden and mycotoxin contamination, maize marketed in the metropolitan region of Asuncion constitutes a potential risk to food safety and public health and requires control measures.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/microorganisms11081879
Formato Recurso	PDF
Palabras Claves	Aspergillus flavus food safety MALDI-TOF MS mycotoxins mycotoxin co-occurrence polyphasic approach
Ubicación del archivo	http://dx.doi.org/10.3390/microorganisms11081879
Categoría OCDE	Microbiología
Materias	Aspergillus flavus Seguridad alimenticia EM MALDI-TOF micotoxinas coexistencia de micotoxinas enfoque polifásico
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001056988100001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)	CONACYT 14-INV-301 ANID-FONDECYT 1221024 ANID FONDECYT 1221024