Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asuncion, Paraguay
| Primer Autor |
Arrua, Andrea
|
| Co-autores |
Moura-Mendes, Juliana
Cazal-Martinez, Cinthia C.
Rojas, Cinthia
Ferreira, Francisco
Perez-Estigarribia, Pastor
Dias, Nathalia
Godoy, Patricio
Costa, Jessica
Santos, Cledir
|
| Título |
Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asuncion, Paraguay
|
| Editorial |
MDPI
|
| Revista |
MICROORGANISMS
|
| Lenguaje |
en
|
| Resumen |
Zea mays var. amylacea and Zea mays var. indurata are maize ecotypes from Paraguay. Aspergillus section Flavi is the main spoilage fungus of maize under storage conditions. Due to its large intraspecific genetic variability, the accurate identification of this fungal taxonomic group is difficult. In the present study, potential mycotoxigenic strains of Aspergillus section Flavi isolated from Z. mays var. indurata and Z. mays var. amylacea that are marketed in the metropolitan region of Asuncion were identified by a polyphasic approach. Based on morphological characters, 211 isolates were confirmed to belong to Aspergillus section Flavi. A subset of 92 strains was identified as Aspergillus flavus by mass spectrometry MALDI-TOF and the strains were classified by MALDI-TOF MS into chemotypes based on their aflatoxins and cyclopiazonic acid production. According to the partial sequencing of ITS and CaM genes, a representative subset of 38 A. flavus strains was confirmed. Overall, 75 A. flavus strains (86%) were characterized as producers of aflatoxins. The co-occurrence of at least two mycotoxins (AF/ZEA, FUM/ZEA, and AF/ZEA/FUM) was detected for five of the Z. mays samples (63%). Considering the high mycological bioburden and mycotoxin contamination, maize marketed in the metropolitan region of Asuncion constitutes a potential risk to food safety and public health and requires control measures.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.3390/microorganisms11081879
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Aspergillus flavus
food safety
MALDI-TOF MS
mycotoxins
mycotoxin co-occurrence
polyphasic approach
|
| Ubicación del archivo | |
| Categoría OCDE |
Microbiología
|
| Materias |
Aspergillus flavus
Seguridad alimenticia
EM MALDI-TOF
micotoxinas
coexistencia de micotoxinas
enfoque polifásico
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| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001056988100001
|
| Tipo de ruta |
verde# dorado
|
| Categoría WOS |
Microbiología
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
CONACYT 14-INV-301
ANID-FONDECYT 1221024
ANID FONDECYT 1221024
|
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