Repositorio UFRO · Omeka S

The salivary peptide histatin-1 enhances bone repair in vivo

Primer Autor	Torres, Pedro
Co-autores	Flores, Victor Flores, Tania Silva, Patricio Gonzalez, Luis Cordova, Luis A. Reyes, Montserrat Torres, Vicente A.
Título	The salivary peptide histatin-1 enhances bone repair in vivo
Editorial	ACADEMIC PRESS INC ELSEVIER SCIENCE
Revista	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Lenguaje	en
Resumen	The salivary peptide histatin-1 was recently described as a novel osteogenic factor that stimulates cell adhesion, migration, and differentiation in bone-lineage cells. Since these cell responses collectively contribute to bone regeneration, we hypothesized that histatin-1 harbors the capacity to enhance bone tissue repair at the preclinical level. By using a model of monocortical bone defect, we explored the effects of histatin-1 in tibial mineralization and organic matrix formation in vivo. To this end, different amounts of histatin-1 were embedded in one-mm3 collagen sponges and then applied to tibial monocortical defects in C57bl/6 mice. After seven days, mice were euthanized, and samples were processed for subsequent analysis. Micro-computed tomography screening showed that histatin-1 increased intra-osseous mineralization, and this phenomenon was accompanied by augmented collagen matrix deposition and closure of cortical defect edges, as determined by Hematoxylin-Eosin and Masson's Trichrome staining. Moreover, immunohistochemical analyses showed that histatin-1 increased the expression of the osteogenic marker alkaline phosphatase, which was accompanied by augmented blood vessel formation. Collectively, our findings show that histatin-1 itself promotes bone regeneration in an orthotopic model, proposing this molecule as a therapeutic candidate for use in bone regenerative medicine.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.bbrc.2023.07.047
Formato Recurso	PDF
Palabras Claves	Animals Bone Regeneration Cell Differentiation Collagen / metabolism Histatins / pharmacology Mice Osteogenesis Salivary Proteins and Peptides X-Ray Microtomography
Ubicación del archivo	http://dx.doi.org/10.1016/j.bbrc.2023.07.047
Categoría OCDE	Bioquímica y biología molecular Biofísica
Materias	Animales Regeneración Ósea Diferenciación celular Colágeno/metabolismo Histatinas/farmacología Ratones Osteogénesis Proteínas y Péptidos Salivales Microtomografía de rayos X
Página de inicio (Recomendado-único)	207.0
Página final (Recomendado-único)	212
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	metadata
Access Rights	metadata
Id de Web of Science	WOS:001061841500001
ISSN	0006-291X
Categoría WOS	Bioquímica y biología molecular Biofísica
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 1220517 ANID-FONDECYT 11221000 ANID-FONDECYT 11190536 ANID-FONDAP 15130011 ACCDiS ANID-FONDEF ID22I10248 IMII ICN09_016/ICN 2021_045 ANID FONDECYT 1220517 ANID FONDECYT 11221000 ANID FONDECYT 11190536 ANID FONDAP 15130011 ANID FONDEF ID22I10248