The salivary peptide histatin-1 enhances bone repair in vivo
| Primer Autor |
Torres, Pedro
|
| Co-autores |
Flores, Victor
Flores, Tania
Silva, Patricio
Gonzalez, Luis
Cordova, Luis A.
Reyes, Montserrat
Torres, Vicente A.
|
| Título |
The salivary peptide histatin-1 enhances bone repair in vivo
|
| Editorial |
ACADEMIC PRESS INC ELSEVIER SCIENCE
|
| Revista |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
|
| Lenguaje |
en
|
| Resumen |
The salivary peptide histatin-1 was recently described as a novel osteogenic factor that stimulates cell adhesion, migration, and differentiation in bone-lineage cells. Since these cell responses collectively contribute to bone regeneration, we hypothesized that histatin-1 harbors the capacity to enhance bone tissue repair at the preclinical level. By using a model of monocortical bone defect, we explored the effects of histatin-1 in tibial mineralization and organic matrix formation in vivo. To this end, different amounts of histatin-1 were embedded in one-mm3 collagen sponges and then applied to tibial monocortical defects in C57bl/6 mice. After seven days, mice were euthanized, and samples were processed for subsequent analysis. Micro-computed tomography screening showed that histatin-1 increased intra-osseous mineralization, and this phenomenon was accompanied by augmented collagen matrix deposition and closure of cortical defect edges, as determined by Hematoxylin-Eosin and Masson's Trichrome staining. Moreover, immunohistochemical analyses showed that histatin-1 increased the expression of the osteogenic marker alkaline phosphatase, which was accompanied by augmented blood vessel formation. Collectively, our findings show that histatin-1 itself promotes bone regeneration in an orthotopic model, proposing this molecule as a therapeutic candidate for use in bone regenerative medicine.
|
| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
|
| doi |
10.1016/j.bbrc.2023.07.047
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Animals
Bone Regeneration
Cell Differentiation
Collagen / metabolism
Histatins / pharmacology
Mice
Osteogenesis
Salivary Proteins and Peptides
X-Ray Microtomography
|
| Ubicación del archivo | |
| Categoría OCDE |
Bioquímica y biología molecular
Biofísica
|
| Materias |
Animales
Regeneración Ósea
Diferenciación celular
Colágeno/metabolismo
Histatinas/farmacología
Ratones
Osteogénesis
Proteínas y Péptidos Salivales
Microtomografía de rayos X
|
| Página de inicio (Recomendado-único) |
207.0
|
| Página final (Recomendado-único) |
212
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| Derechos de acceso |
metadata
|
| Access Rights |
metadata
|
| Id de Web of Science |
WOS:001061841500001
|
| ISSN |
0006-291X
|
| Categoría WOS |
Bioquímica y biología molecular
Biofísica
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 1220517
ANID-FONDECYT 11221000
ANID-FONDECYT 11190536
ANID-FONDAP 15130011
ACCDiS
ANID-FONDEF ID22I10248
IMII ICN09_016/ICN 2021_045
ANID FONDECYT 1220517
ANID FONDECYT 11221000
ANID FONDECYT 11190536
ANID FONDAP 15130011
ANID FONDEF ID22I10248
|
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