High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
| Primer Autor |
Nova-Lamperti, Estefanía
|
| Co-autores |
Vidal-Seguel, Nicolas
Cabrera, Camilo
Ferrada, Luciano
Artigas-Arias, Macarena
Alegria-Molina, Andrea
Sanhueza, Sergio
Flores, Alejandra
Huard, Nolberto
Sapunar, Jorge
Salazar, Luis A.
McGregor, Reuben
Marzuca-Nassr, Gabriel Nasri
|
| Título |
High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
|
| Editorial |
PERGAMON-ELSEVIER SCIENCE LTD
|
| Revista |
EXPERIMENTAL GERONTOLOGY
|
| Lenguaje |
en
|
| Resumen |
Neutrophil extracellular trap formation (NETosis) is a mechanism used by neutrophils to capture pathogens with their own DNA. However, the exacerbation of this immune response is related to serious inflammatory diseases. Aging is known to lead to an excessive increase in NETosis associated with various diseases. Under this scenario, the search for strategies that regulate the release of NETosis in older people becomes relevant. High-intensity interval training (HIIT) involves repeated bouts of relatively intense exercise with alternating short recovery periods. This training has shown beneficial effects on health parameters during aging and disease. However, little is known about the potential role of HIIT in the regulation of NETosis in healthy older people. The aim of this study was to evaluate the induction of NETosis by serum from healthy young and older men, before and after 12 weeks of HIIT using healthy neutrophils as a biosensor. HIIT was performed 3 times per week for 12 weeks in young (YOUNG, 21 +/- 1 years, BMI 26.01 +/- 2.64 kg center dot m(-2), n = 10) and older men (OLDER, 66 +/- 5 years, BMI 27.43 +/- 3.11 kg center dot m(-2), n = 10). Serum samples were taken before and after the HIIT program and NETosis was measured with live cell imaging in donated neutrophils cultured with serum from the participants for 30 h. Our results showed that serum from older men at baseline induced greater baseline NETosis than younger men (p < 0.05, effect size, >= 0.8), and 12 weeks of HIIT significantly reduced (Interaction Effect, p < 0.05, effect size, 0.134) the induction of NETosis in older men. In conclusion, HIIT is a feasible non-invasive training strategy modulating NETosis induction. Additionally, the use of neutrophils as a biosensor is an effective method for the quantification of NETosis induction in real time.
|
| Fecha Publicación |
2023
|
| Tipo de Recurso |
artículo original
|
| doi |
10.1016/j.exger.2023.112280
|
| Formato Recurso |
PDF
|
| Palabras Claves |
NETs
Neutrophil extracellular trap
High-intensity interval training
Exercise
Elderly
|
| Ubicación del archivo | |
| Categoría OCDE |
Geriatría y Gerontología
|
| Materias |
NET
Trampa extracelular de neutrófilos
Entrenamiento de intervalos de alta intensidad
Ejercicio
Anciano
|
| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY-NC 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY-NC 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001077024700001
|
| ISSN |
0531-5565
|
| Tipo de ruta |
verde# hibrido
|
| Categoría WOS |
Geriatría y Gerontología
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID-FONDECYT 11180949
UFRO DIUFRO DI18-0068
UFRO DIUFRO DI20-2017
ANID-FONDECYT 11170610
ANID-FONDECYT 1211480
ANID PAI79170073
ANID ACT210085
ANID 2021 - 21211236
ANID 2022 - 21220848
ANID FONDECYT 11180949
UFRO DI18-0068
UFRO DI20-2017
ANID FONDECYT 11170610
ANID FONDECYT 1211480
|
cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl