Repositorio UFRO · Omeka S

High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor

Primer Autor	Nova-Lamperti, Estefanía
Co-autores	Vidal-Seguel, Nicolas Cabrera, Camilo Ferrada, Luciano Artigas-Arias, Macarena Alegria-Molina, Andrea Sanhueza, Sergio Flores, Alejandra Huard, Nolberto Sapunar, Jorge Salazar, Luis A. McGregor, Reuben Marzuca-Nassr, Gabriel Nasri
Título	High-intensity interval training reduces the induction of neutrophil extracellular traps in older men using live-neutrophil imaging as biosensor
Editorial	PERGAMON-ELSEVIER SCIENCE LTD
Revista	EXPERIMENTAL GERONTOLOGY
Lenguaje	en
Resumen	Neutrophil extracellular trap formation (NETosis) is a mechanism used by neutrophils to capture pathogens with their own DNA. However, the exacerbation of this immune response is related to serious inflammatory diseases. Aging is known to lead to an excessive increase in NETosis associated with various diseases. Under this scenario, the search for strategies that regulate the release of NETosis in older people becomes relevant. High-intensity interval training (HIIT) involves repeated bouts of relatively intense exercise with alternating short recovery periods. This training has shown beneficial effects on health parameters during aging and disease. However, little is known about the potential role of HIIT in the regulation of NETosis in healthy older people. The aim of this study was to evaluate the induction of NETosis by serum from healthy young and older men, before and after 12 weeks of HIIT using healthy neutrophils as a biosensor. HIIT was performed 3 times per week for 12 weeks in young (YOUNG, 21 +/- 1 years, BMI 26.01 +/- 2.64 kg center dot m(-2), n = 10) and older men (OLDER, 66 +/- 5 years, BMI 27.43 +/- 3.11 kg center dot m(-2), n = 10). Serum samples were taken before and after the HIIT program and NETosis was measured with live cell imaging in donated neutrophils cultured with serum from the participants for 30 h. Our results showed that serum from older men at baseline induced greater baseline NETosis than younger men (p < 0.05, effect size, >= 0.8), and 12 weeks of HIIT significantly reduced (Interaction Effect, p < 0.05, effect size, 0.134) the induction of NETosis in older men. In conclusion, HIIT is a feasible non-invasive training strategy modulating NETosis induction. Additionally, the use of neutrophils as a biosensor is an effective method for the quantification of NETosis induction in real time.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.exger.2023.112280
Formato Recurso	PDF
Palabras Claves	NETs Neutrophil extracellular trap High-intensity interval training Exercise Elderly
Ubicación del archivo	http://dx.doi.org/10.1016/j.exger.2023.112280
Categoría OCDE	Geriatría y Gerontología
Materias	NET Trampa extracelular de neutrófilos Entrenamiento de intervalos de alta intensidad Ejercicio Anciano
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY-NC 4.0
Condición de la licencia (Recomendado-repetible)	CC BY-NC 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001077024700001
ISSN	0531-5565
Tipo de ruta	verde# hibrido
Categoría WOS	Geriatría y Gerontología
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 11180949 UFRO DIUFRO DI18-0068 UFRO DIUFRO DI20-2017 ANID-FONDECYT 11170610 ANID-FONDECYT 1211480 ANID PAI79170073 ANID ACT210085 ANID 2021 - 21211236 ANID 2022 - 21220848 ANID FONDECYT 11180949 UFRO DI18-0068 UFRO DI20-2017 ANID FONDECYT 11170610 ANID FONDECYT 1211480