Repositorio
Institucional

Barra, Patricio J.

Rodriguez, Rodrigo

Larama, Giovanni

Carrion, Victor J.

Mora, Maria de la Luz

Hale, Lauren

Duran, Paola

Microbiome engineering optimized by Antarctic microbiota to support a plant host under water deficit

FRONTIERS MEDIA SA

FRONTIERS IN PLANT SCIENCE

en

Climate change challenges modern agriculture to develop alternative and eco-friendly solutions to alleviate abiotic and/or biotic stresses. The use of soil microbiomes from extreme environments opens new avenues to discover novel microorganisms and microbial functions to protect plants. In this study we confirm the ability of a bioinoculant, generated by natural engineering, to promote host development under water stress. Microbiome engineering was mediated through three factors i) Antarctic soil donation, ii) water deficit and iii) multigenerational tomato host selection. We revealed that tomato plants growing in soils supplemented with Antarctic microbiota were tolerant to water deficit stress after 10 generations. A clear increase in tomato seedling tolerance against water deficit stress was observed in all soils over generations of Host Mediated Microbiome Engineering, being Fildes mixture the most representatives, which was evidenced by an increased survival time, plant stress index, biomass accumulation, and decreased leaf proline content. Microbial community analysis using 16s rRNA gene amplicon sequencing data suggested a microbiome restructuring that could be associated with increased tolerance of water deficit. Additionally, the results showed a significant increase in the relative abundance of Candidatus Nitrosocosmicus and Bacillus spp. which could be key taxa associated with the observed tolerance improvement. We proposed that in situ microbiota engineering through the evolution of three factors (long-standing extreme climate adaption and host and stress selection) could represent a promising strategy for novel generation of microbial inoculants.

2023

artículo original

10.3389/fpls.2023.1241612

PDF

Antarctic microbiome

sustainable agriculture

climate change

microbiome transplant

water deficit stress

Host Mediated Microbiota Selection (HMMS)

extreme environment

Ciencias de las plantas

Microbioma antártico

Agricultura sostenible

cambio climático

trasplante de microbioma

estrés por déficit hídrico

Selección de microbiota mediada por el huésped (HMMS)

medio ambiente extremo

artículo original

versión publicada

CC BY 4.0

CC BY 4.0

acceso abierto

acceso abierto

WOS:001075326400001

1664-462X

verde# dorado

Ciencias de las plantas

INACH RT_06-17

ANID-FONDECYT 1201196

ANID-FONDECYT 11200377

ANID-FONDECYT 3230478

ANID FONDECYT 1201196

ANID 11200377

ANID FONDECYT 3230478