Repositorio UFRO · Omeka S

The efficient activity of plant essential oils for inhibiting Botrytis cinerea and Penicillium expansum: Mechanistic insights into antifungal activity

Primer Autor	Fincheira, Paola
Co-autores	Jofre, Ignacio Espinoza, Javier Levio-Raiman, Marcela Tortella, Gonzalo Oliveira, Halley Caixeta Diez, Maria Cristina Quiroz, Andres Rubilar, Olga
Título	The efficient activity of plant essential oils for inhibiting Botrytis cinerea and Penicillium expansum: Mechanistic insights into antifungal activity
Editorial	ELSEVIER GMBH
Revista	MICROBIOLOGICAL RESEARCH
Lenguaje	en
Resumen	Botrytis cinerea and Penicillium expansum produce deterioration in fruit quality, causing losses to the food industry. Thus, plant essential oils (EOs) have been proposed as a sustainable alternative for minimizing the application of synthetic fungicides due to their broad-spectrum antifungal properties. This study investigated the efficacy of five EOs in suppressing the growth of B. cinerea and P. expansum and their potential antifungal mechanisms. EOs of Mentha x piperita L., Origanum vulgare L., Thymus vulgaris L., Eucalyptus globules Labill., and Lavandula angustifolia Mill., were screened for both fungi. The results showed that the EO of T. vulgaris and O. vulgare were the most efficient in inhibiting the growth of B. cinerea and P. expansum. The concentration increase of all EO tested increased fungi growth inhibition. Exposure of fungi to EOs of T. vulgaris and O. vulgare increased the pH and the release of constituents absorbing 260 nm and soluble proteins, reflecting membrane permeability alterations. Fluorescence microscopic examination revealed that tested EOs produce structural alteration in cell wall component deposition, decreasing the hypha width. Moreover, propidium iodide and Calcein-AM stains evidenced the loss of membrane integrity and reduced cell viability of fungi treated with EOs. Fungi treated with EOs decreased the mitochondria activity and the respiratory process. Therefore, these EOs are effective antifungal agents against B. cinerea and P. expansum, which is attributed to changes in the cell wall structure, the breakdown of the cell membrane, and the alteration of the mitochondrial activity.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.micres.2023.127486
Formato Recurso	PDF
Palabras Claves	Plant essential oil Fungal pathogen inhibition Mitochondria activity Cell membrane fluidity
Ubicación del archivo	http://dx.doi.org/10.1016/j.micres.2023.127486
Categoría OCDE	Microbiología
Materias	Aceite esencial de plantas Inhibición de patógenos fúngicos Actividad mitocondrial Fluidez de la membrana celular
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY-NC-ND 4.0
Condición de la licencia (Recomendado-repetible)	CC BY-NC-ND 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001081318000001
ISSN	0944-5013
Tipo de ruta	Verde# dorado
Categoría WOS	Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 11220070 ANID-FONDAP 15130015 ANID FOVI220003 ANID InES19-FRO19001 ANID FONDECYT 11220070 ANID FONDAP 15130015