Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Primer Autor
Briceno, Gabriela
Co-autores
Donoso-Pinol, Pamela
Evaristo, Joseph A. M.
Nogueira, Fabio C. S.
Leiva, Barbara
Lamilla, Claudio
Schalchli, Heidi
Diez, Maria Cristina
Título
Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation
Editorial
MDPI
Revista
MICROORGANISMS
Lenguaje
en
Resumen
The fungicide iprodione (IPR) (3-(3,5-dichlorophenyl) N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide) is a highly toxic compound. Although IPR has been restricted, it is still being applied in many places around the world, constituting an environmental risk. The biodegradation of IPR is an attractive option for reducing its residues. In this study, we isolated thirteen IPR-tolerant bacteria from a biopurification system designed to treat pesticides. A study of biodegradation using different strains was comparatively evaluated, and the best degradation rate of IPR was presented by Achromobacter sp. C1 with a half-life (T1/2) of 9 days. Based on a nano-LC-MS/MS analysis for the strains, proteins solely expressed in the IPR treatment were identified by highlighting the strain Achromobacter sp. C1, with 445 proteins primarily involved in the biosynthesis of secondary metabolites and microbial metabolism in diverse environments. Differentially expressed protein amidases were involved in six metabolic pathways. Interestingly, formamidase was inhibited while other cyclases, i.e., amidase and mandelamide hydrolase, were overexpressed, thereby minimizing the effect of IPR on the metabolism of strain C1. The dynamic changes in the protein profiles of bacteria that degrade IPR have been poorly studied, therefore, our results offer new insight into the metabolism of IPR-degrading microorganisms, with special attention paid to amidases.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/microorganisms11102367
Formato Recurso
PDF
Palabras Claves
pesticide degradation
amidase
proteome
pesticide-tolerant bacteria
Ubicación del archivo
http://dx.doi.org/10.3390/microorganisms11102367
Categoría OCDE
Microbiología
Materias
degradación de pesticidas
amidasa
proteoma
bacterias tolerantes a los pesticidas
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:001093617100001
Tipo de ruta
verde# dorado
Categoría WOS
Microbiología
Colecciones
Colección Publicaciones Científicas
Colección ANID
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