Repositorio UFRO · Omeka S

Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation

Primer Autor	Briceno, Gabriela
Co-autores	Donoso-Pinol, Pamela Evaristo, Joseph A. M. Nogueira, Fabio C. S. Leiva, Barbara Lamilla, Claudio Schalchli, Heidi Diez, Maria Cristina
Título	Metabolic Profiling and Comparative Proteomic Insight in Respect of Amidases during Iprodione Biodegradation
Editorial	MDPI
Revista	MICROORGANISMS
Lenguaje	en
Resumen	The fungicide iprodione (IPR) (3-(3,5-dichlorophenyl) N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide) is a highly toxic compound. Although IPR has been restricted, it is still being applied in many places around the world, constituting an environmental risk. The biodegradation of IPR is an attractive option for reducing its residues. In this study, we isolated thirteen IPR-tolerant bacteria from a biopurification system designed to treat pesticides. A study of biodegradation using different strains was comparatively evaluated, and the best degradation rate of IPR was presented by Achromobacter sp. C1 with a half-life (T1/2) of 9 days. Based on a nano-LC-MS/MS analysis for the strains, proteins solely expressed in the IPR treatment were identified by highlighting the strain Achromobacter sp. C1, with 445 proteins primarily involved in the biosynthesis of secondary metabolites and microbial metabolism in diverse environments. Differentially expressed protein amidases were involved in six metabolic pathways. Interestingly, formamidase was inhibited while other cyclases, i.e., amidase and mandelamide hydrolase, were overexpressed, thereby minimizing the effect of IPR on the metabolism of strain C1. The dynamic changes in the protein profiles of bacteria that degrade IPR have been poorly studied, therefore, our results offer new insight into the metabolism of IPR-degrading microorganisms, with special attention paid to amidases.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/microorganisms11102367
Formato Recurso	PDF
Palabras Claves	pesticide degradation amidase proteome pesticide-tolerant bacteria
Ubicación del archivo	http://dx.doi.org/10.3390/microorganisms11102367
Categoría OCDE	Microbiología
Materias	degradación de pesticidas amidasa proteoma bacterias tolerantes a los pesticidas
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001093617100001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología