Effect of different sperm populations on neutrophils extracellular traps (NETs) formation in cattle

Primer Autor
Zambrano, Fabiola
Co-autores
Rivera-Concha, Rodrigo
Moya, Claudia
Leon, Marion
Uribe, Pamela
Schulz, Mabel
Prado, Aurora
Taubert, Anja
Hermosilla, Carlos
Sanchez, Raul
Título
Effect of different sperm populations on neutrophils extracellular traps (NETs) formation in cattle
Editorial
ELSEVIER SCI LTD
Revista
RESEARCH IN VETERINARY SCIENCE
Lenguaje
en
Resumen
In cattle, clinical and subclinical inflammation in the bovine female reproductive tract (FRT) significantly reduces fertility. PMN participate in this FRT-associated inflammation by eliminating pathogens by eliciting various defense mechanisms, with the release of neutrophil extracellular traps NETs) being the latest process discovered. Consistently, human-, bovine-and porcine-derived spermatozoa induce release of NETs in exposed PMN of the same species origin, and thereby decreasing sperm motility through NETs-mediated entrapment. The release of NETs in the presence of different sperm sub-populations is evaluated in this work. Cryopreserved bovine sperm were selected and different sperm populations were used: viable sperm, sperm with oxidative stress, capacitated sperm, and sperm with loss of viability. Isolated PMN of dairy cows were co-incubated with these sperm populations for 4 h. Neutrophil elastase (NE) and DNA were detected by fluorescence microscopy analysis. It was noted that exposed bovine PMN released NETs in the presence of sperm. Moreover, spermtriggered NETosis resulted different phenotypes of NETs, i. e. spread NETs (sprNETs), diffused NETs (diffNETs) and aggregated NETs (aggNETs). Viable/motile spermatozoa induced a higher proportion of NETotic cells at 15, 60 and 120 min in comparison to controls. In conclusion, all bovine sperm populations in co-culture with PMN generated NETs extrusion while viable sperm activated NETotic cells to a greater extent. With this being an early event in the activation of bovine PMN.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.1016/j.rvsc.2023.105028
Formato Recurso
PDF
Palabras Claves
Bovine
Spermatozoa
Neutrophil extracellular traps
Sperm motility
Ubicación del archivo
http://dx.doi.org/10.1016/j.rvsc.2023.105028
Categoría OCDE
Ciencias Veterinarias
Materias
Bovino
Espermatozoide
Trampas extracelulares de neutrófilos
Motilidad de los espermatozoides
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
Derechos de acceso
metadata
Access Rights
metadata
Id de Web of Science
WOS:001106482700001
ISSN
0034-5288
Tipo de ruta
verde / hibrida
Categoría WOS
Ciencias Veterinarias
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 11200955
ANID 21202442
UFRO DI22-3034
UFRO DI23-3007
ANID FONDECYT 11200955
ANID 2020 21202442
Colecciones
Colección Publicaciones Científicas
Colección ANID
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