Repositorio UFRO · Omeka S

Effect of different sperm populations on neutrophils extracellular traps (NETs) formation in cattle

Primer Autor	Zambrano, Fabiola
Co-autores	Rivera-Concha, Rodrigo Moya, Claudia Leon, Marion Uribe, Pamela Schulz, Mabel Prado, Aurora Taubert, Anja Hermosilla, Carlos Sanchez, Raul
Título	Effect of different sperm populations on neutrophils extracellular traps (NETs) formation in cattle
Editorial	ELSEVIER SCI LTD
Revista	RESEARCH IN VETERINARY SCIENCE
Lenguaje	en
Resumen	In cattle, clinical and subclinical inflammation in the bovine female reproductive tract (FRT) significantly reduces fertility. PMN participate in this FRT-associated inflammation by eliminating pathogens by eliciting various defense mechanisms, with the release of neutrophil extracellular traps NETs) being the latest process discovered. Consistently, human-, bovine-and porcine-derived spermatozoa induce release of NETs in exposed PMN of the same species origin, and thereby decreasing sperm motility through NETs-mediated entrapment. The release of NETs in the presence of different sperm sub-populations is evaluated in this work. Cryopreserved bovine sperm were selected and different sperm populations were used: viable sperm, sperm with oxidative stress, capacitated sperm, and sperm with loss of viability. Isolated PMN of dairy cows were co-incubated with these sperm populations for 4 h. Neutrophil elastase (NE) and DNA were detected by fluorescence microscopy analysis. It was noted that exposed bovine PMN released NETs in the presence of sperm. Moreover, spermtriggered NETosis resulted different phenotypes of NETs, i. e. spread NETs (sprNETs), diffused NETs (diffNETs) and aggregated NETs (aggNETs). Viable/motile spermatozoa induced a higher proportion of NETotic cells at 15, 60 and 120 min in comparison to controls. In conclusion, all bovine sperm populations in co-culture with PMN generated NETs extrusion while viable sperm activated NETotic cells to a greater extent. With this being an early event in the activation of bovine PMN.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.1016/j.rvsc.2023.105028
Formato Recurso	PDF
Palabras Claves	Bovine Spermatozoa Neutrophil extracellular traps Sperm motility
Ubicación del archivo	http://dx.doi.org/10.1016/j.rvsc.2023.105028
Categoría OCDE	Ciencias Veterinarias
Materias	Bovino Espermatozoide Trampas extracelulares de neutrófilos Motilidad de los espermatozoides
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
Derechos de acceso	metadata
Access Rights	metadata
Id de Web of Science	WOS:001106482700001
ISSN	0034-5288
Tipo de ruta	verde / hibrida
Categoría WOS	Ciencias Veterinarias
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID-FONDECYT 11200955 ANID 21202442 UFRO DI22-3034 UFRO DI23-3007 ANID FONDECYT 11200955 ANID 2020 21202442