Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence

Primer Autor
Arias, Maria Elena
Co-autores
Valencia, Cecilia
Perez-Garcia, Felipe
Aguila, Luis
Felmer, Ricardo
Título
Combined Exogenous Activation of Bovine Oocytes: Effects on Maturation-Promoting Factor, Mitogen-Activated Protein Kinases, and Embryonic Competence
Editorial
MDPI
Revista
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Lenguaje
en
Resumen
Oocyte activation via dual inhibition of protein synthesis and phosphorylation has improved in vitro embryo production in different mammalian species. In this study, we evaluated the effects of the combination of cycloheximide (CHX), dimethyl amino purine (DMAP), and anisomycin (ANY) on the activation of bovine oocytes, particularly on dynamics of MPF and MAPKs, embryonic developmental potential, and quality. The results showed that the cleavage and blastocyst rates, as well as levels of CCNB1, CDK1, p-CDK1(Thr161), and p-CDK1(Thr14-Tyr15), were similar among groups, ANY and ANY + CHX reduced the expression of ERK1/2 compared to DMAP-combinations (p < 0.05), whereas ANY + DMAP, CHX + DMAP, and ANY + CHX + DMAP reduced p-ERK1/2 compared to ANY and ANY + CHX treatments (p < 0.05). The quality of blastocysts in terms of cell counts, their allocation, and the numbers of TUNEL-positive cells did not differ among groups. However, transcript levels of POU5F1 were higher in embryos derived from ANY + CHX + DMAP treatment compared to other groups, while expression levels of CDX2 did not show differences. In addition, the BCL2A1/BAX ratio of the ANY + CHX + DMAP treatment was significantly low compared to the ANY treatment (p < 0.05) and did not differ significantly from the other treatments. In conclusion, oocyte activation by dual inhibition of protein synthesis and phosphorylation induces MPF inactivation without degradation of CCNB1, while MAPK inactivation occurs differentially between these inhibitors. Thus, although the combined use of these inhibitors does not affect early developmental competence in vitro, it positively impacts the expression of transcripts associated with embryonic quality.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/ijms242115794
Formato Recurso
PDF
Palabras Claves
parthenogenesis
inhibitors
anisomycin
cycloheximide
DMAP
MPF
MAPKs
CDX2
Ubicación del archivo
Categoría OCDE
Bioquímica y biología molecular
Química
Materias
partenogénesis
inhibidores
anisomicina
cicloheximida
DMAP
MPF
MAPK
CDX2
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:001103359100001
ISSN
1661-6596
Tipo de ruta
verde# dorado
Categoría WOS
Bioquímica y biología molecular
Química
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID-FONDECYT 1181453
ANID 21181741
UFRO PDT21-0001
ANID FONDECYT 1181453
UFRO PDT21-0001
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