Repositorio UFRO · Omeka S

Bacterial Community Composition and Presence of Plasmids in the Endosphere- and Rhizosphere-Associated Microbiota of Sea Fig (Carpobrotus aequilaterus)

Primer Autor	Jorquera, Milko A.
Co-autores	Sanchez-Salazar, Angela M. Acuna, Jacquelinne J. Sadowsky, Michael J.
Título	Bacterial Community Composition and Presence of Plasmids in the Endosphere- and Rhizosphere-Associated Microbiota of Sea Fig (Carpobrotus aequilaterus)
Editorial	MDPI
Revista	DIVERSITY-BASEL
Lenguaje	en
Resumen	The plant microbiome is one of the most important environments for ecological interactions between bacteria that impact the plant and the ecosystem. However, studies on the diversity of mobile genetic elements (such as plasmids) associated with the plant microbiome are very scarce. Here, we determined the bacterial community composition and the occurrence of plasmids in the microbiota associated with sea fig, Carpobrotus aequilaterus (N.E. Br.), a succulent species widely used as an ornamental plant in Chile. The abundance and composition of the endophytic and rhizospheric bacterial communities were determined by quantitative PCR (qPCR) and DNA metabarcoding analysis. Plasmid diversity in the plant microbiome was determined by plasmid DNA extraction and screened by endpoint PCR of backbone genes for four different incompatibility groups (Inc). The results showed about 106 copies of the 16S rRNA gene in the endosphere and rhizosphere, showing significant differences according to the diversity index. Proteobacteria (Pseudomonadota, 43.4%), Actinobacteria (Actinomycetota, 25.7%), and Bacteroidetes (Bacteroidota, 17.4%) were the most dominant taxa in both plant compartments, and chemoheterotrophy (30%) was the main predicted function assigned to the microbiota. Plasmid diversity analysis showed the presence of transferable plasmids in the endosphere and rhizosphere of C. aequilaterus, particularly among environmental plasmids belonging to the IncP and IncN incompatibility groups.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/d15111156
Formato Recurso	PDF
Palabras Claves	Carpobrotus aequilaterus sea fig Chilean pigface endosphere plasmids ornamental plant plant microbiome rhizosphere
Ubicación del archivo	http://dx.doi.org/10.3390/d15111156
Categoría OCDE	Biodiversidad y Conservación Ciencias Ambientales y Ecología
Materias	Carpobrotus aequilaterus higo de mar cara de cerdo chilena endosfera plásmidos planta ornamental microbioma vegetal rizosfera
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001120177000001
Tipo de ruta	verde# dorado
Categoría WOS	Biodiversidad y Conservación Ciencias Ambientales y Ecología
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID