Repositorio UFRO · Omeka S

Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides

Primer Autor	Galetovic, Alexandra
Co-autores	Pena, Gabriel Fernandez, Nicole Urrutia, Milton Flores, Nataly Gomez-Silva, Benito Di Ruggiero, Jocelyne Shene, Carolina Bustamante, Mariela
Título	Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides
Editorial	MDPI
Revista	MICROORGANISMS
Lenguaje	en
Resumen	Cyanobacteria produce exopolysaccharides (EPSs) as an adaptative mechanism against ultraviolet radiation and desiccation. Cellulose is present in the extracellular polymeric substance in some cyanobacteria genera and it has been proposed as a raw material for biofuel production. The goal of this work was to evaluate the cellulose presence in EPS of Atacama cyanobacteria strains and its use as an alternative and innovative biological source to produce bioethanol. The presence of cellulose was evaluated using techniques of molecular biology, bioinformatics, and electronic microscopy. The conserved motif D,D,D,35QXXRW, characteristic of processive beta-glycosyltransferase in all cellulose-producing organisms, was identified in the genome of the LLA-10 strain. This is evidence that cellulose synthase in the LLA-10 strain is a functional enzyme. EPS from Atacama cyanobacteria was hydrolyzed by beta-glucosidases (cellobiase and cellulase) and the released glucose was yeast-fermented to ethanol. Ethanol production reached 172.69 +/- 0.02 mg ethanol/g EPS after 48 h of incubation. These results are the first step in the evaluation of EPS produced by native cyanobacteria isolated from northern Chile for future biotechnological applications such as the production of bioethanol.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3390/microorganisms11112668
Formato Recurso	PDF
Palabras Claves	cyanobacteria cellulose bioethanol exopolysaccharides cellulose synthase
Ubicación del archivo	http://dx.doi.org/10.3390/microorganisms11112668
Categoría OCDE	Microbiología
Materias	cianobacterias celulosa bioetanol exopolisacáridos celulosa sintasa
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001113721300001
Tipo de ruta	verde# dorado
Categoría WOS	Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)	FIC-R 4603 IANAS UAnt ANT20992 CeBiB FB-0001 ANID ICN2021_044 UA SI-5305 UA ANT20992