Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides

Primer Autor
Galetovic, Alexandra
Co-autores
Pena, Gabriel
Fernandez, Nicole
Urrutia, Milton
Flores, Nataly
Gomez-Silva, Benito
Di Ruggiero, Jocelyne
Shene, Carolina
Bustamante, Mariela
Título
Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides
Editorial
MDPI
Revista
MICROORGANISMS
Lenguaje
en
Resumen
Cyanobacteria produce exopolysaccharides (EPSs) as an adaptative mechanism against ultraviolet radiation and desiccation. Cellulose is present in the extracellular polymeric substance in some cyanobacteria genera and it has been proposed as a raw material for biofuel production. The goal of this work was to evaluate the cellulose presence in EPS of Atacama cyanobacteria strains and its use as an alternative and innovative biological source to produce bioethanol. The presence of cellulose was evaluated using techniques of molecular biology, bioinformatics, and electronic microscopy. The conserved motif D,D,D,35QXXRW, characteristic of processive beta-glycosyltransferase in all cellulose-producing organisms, was identified in the genome of the LLA-10 strain. This is evidence that cellulose synthase in the LLA-10 strain is a functional enzyme. EPS from Atacama cyanobacteria was hydrolyzed by beta-glucosidases (cellobiase and cellulase) and the released glucose was yeast-fermented to ethanol. Ethanol production reached 172.69 +/- 0.02 mg ethanol/g EPS after 48 h of incubation. These results are the first step in the evaluation of EPS produced by native cyanobacteria isolated from northern Chile for future biotechnological applications such as the production of bioethanol.
Fecha Publicación
2023
Tipo de Recurso
artículo original
doi
10.3390/microorganisms11112668
Formato Recurso
PDF
Palabras Claves
cyanobacteria
cellulose
bioethanol
exopolysaccharides
cellulose synthase
Ubicación del archivo
http://dx.doi.org/10.3390/microorganisms11112668
Categoría OCDE
Microbiología
Materias
cianobacterias
celulosa
bioetanol
exopolisacáridos
celulosa sintasa
Identificador del recurso (Mandatado-único)
artículo original
Versión del recurso (Recomendado-único)
versión publicada
License
CC BY 4.0
Condición de la licencia (Recomendado-repetible)
CC BY 4.0
Derechos de acceso
acceso abierto
Access Rights
acceso abierto
Id de Web of Science
WOS:001113721300001
Tipo de ruta
verde# dorado
Categoría WOS
Microbiología
Referencia del Financiador (Mandatado si es aplicable-repetible)
FIC-R 4603
IANAS
UAnt ANT20992
CeBiB FB-0001
ANID ICN2021_044
UA SI-5305
UA ANT20992
Colecciones
Colección Publicaciones Científicas
Colección ANID
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