Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables
| Primer Autor |
Jorquera, Milko A.
|
| Co-autores |
Acuna, Jacquelinne J.
Hu, Jingming
Inostroza, Nitza G.
Valenzuela, Tamara
Perez, Pablo
Epstein, Slava
Sessitsch, Angela
Zhang, Qian
|
| Título |
Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables
|
| Editorial |
NATURE PORTFOLIO
|
| Revista |
SCIENTIFIC REPORTS
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| Lenguaje |
en
|
| Resumen |
Chile is a prominent seed exporter globally, but the seed microbiome of vegetables (46% of seeds) and its role in the early stages of plant growth have remained largely unexplored. Here, we employed DNA metabarcoding analysis to investigate the composition and putative functions of endophytic bacterial communities in ungerminated and germinated seeds of the commercial vegetables Apiaceae (parsley and carrot), Asteraceae (lettuce), Brassicaceae (cabbage and broccoli), and Solanaceae (tomato). Bacterial quantification showed 104 to 108 copies of the 16S rRNA gene per gram of ungerminated and germinated seeds. Alpha diversity analysis (e.g., Chao1, Shannon, and Simpson indices) did not indicate significant differences (Kruskal-Wallis test) between ungerminated and germinated seeds, except for Solanaceae. However, beta diversity (PCoA) analysis showed distinctions (Adonis test) between ungerminated and germinated seeds, except Apiaceae. Pseudomonadota and Bacillota were identified as the dominant and specialist taxa in both ungerminated and germinated seed samples. Chemoheterotrophy and fermentation were predicted as the main microbial functional groups in the endophytic bacterial community. Notably, a considerable number of the 143 isolated endophytic strains displayed plant growth-promoting traits (10 to 64%) and biocontrol activity (74% to 82%) against plant pathogens (Xanthomonas and Pseudomonas). This study revealed the high variability in the abundance, diversity, composition, and functionality of endophytic bacteria between ungerminated and germinated seeds in globally commercialized vegetables. Furthermore, potential beneficial endophytic bacteria contained in their seed microbiomes that may contribute to the microbiome of the early stages, development, growth and progeny of vegetables were found.
|
| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
|
| Derecho de Acceso |
acceso abierto
|
| doi |
10.1038/s41598-023-47099-4
|
| Formato Recurso |
PDF
|
| Palabras Claves |
Applied microbiology
Microbial communities
Plant biotechnology
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| Ubicación del archivo | |
| Categoría OCDE |
Ciencia y tecnología: otros temas
|
| Materias |
Microbiología aplicada
Comunidades microbianas
Biotecnología vegetal
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| Identificador del recurso (Mandatado-único) |
artículo original
|
| Versión del recurso (Recomendado-único) |
versión publicada
|
| License |
CC BY 4.0
|
| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
|
| Access Rights |
acceso abierto
|
| Id de Web of Science |
WOS:001104793000064
|
| ISSN |
2045-2322
|
| Tipo de ruta |
verde# dorada
|
| Categoría WOS |
Ciencia y tecnología: otros temas
|
| Referencia del Financiador (Mandatado si es aplicable-repetible) |
ANID 1201386
ANID 1221228
ANID 21210306
ANID 21211278
NSFC 42077386
UFRO DI19-2016
ANID FONDECYT 1201386
ANID FONDECYT 1221228
ANID ICM ICN2021_044
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