Repositorio UFRO · Omeka S

Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables

Primer Autor	Jorquera, Milko A.
Co-autores	Acuna, Jacquelinne J. Hu, Jingming Inostroza, Nitza G. Valenzuela, Tamara Perez, Pablo Epstein, Slava Sessitsch, Angela Zhang, Qian
Título	Endophytic bacterial communities in ungerminated and germinated seeds of commercial vegetables
Editorial	NATURE PORTFOLIO
Revista	SCIENTIFIC REPORTS
Lenguaje	en
Resumen	Chile is a prominent seed exporter globally, but the seed microbiome of vegetables (46% of seeds) and its role in the early stages of plant growth have remained largely unexplored. Here, we employed DNA metabarcoding analysis to investigate the composition and putative functions of endophytic bacterial communities in ungerminated and germinated seeds of the commercial vegetables Apiaceae (parsley and carrot), Asteraceae (lettuce), Brassicaceae (cabbage and broccoli), and Solanaceae (tomato). Bacterial quantification showed 104 to 108 copies of the 16S rRNA gene per gram of ungerminated and germinated seeds. Alpha diversity analysis (e.g., Chao1, Shannon, and Simpson indices) did not indicate significant differences (Kruskal-Wallis test) between ungerminated and germinated seeds, except for Solanaceae. However, beta diversity (PCoA) analysis showed distinctions (Adonis test) between ungerminated and germinated seeds, except Apiaceae. Pseudomonadota and Bacillota were identified as the dominant and specialist taxa in both ungerminated and germinated seed samples. Chemoheterotrophy and fermentation were predicted as the main microbial functional groups in the endophytic bacterial community. Notably, a considerable number of the 143 isolated endophytic strains displayed plant growth-promoting traits (10 to 64%) and biocontrol activity (74% to 82%) against plant pathogens (Xanthomonas and Pseudomonas). This study revealed the high variability in the abundance, diversity, composition, and functionality of endophytic bacteria between ungerminated and germinated seeds in globally commercialized vegetables. Furthermore, potential beneficial endophytic bacteria contained in their seed microbiomes that may contribute to the microbiome of the early stages, development, growth and progeny of vegetables were found.
Fecha Publicación	2023
Tipo de Recurso	artículo original
Derecho de Acceso	acceso abierto
doi	10.1038/s41598-023-47099-4
Formato Recurso	PDF
Palabras Claves	Applied microbiology Microbial communities Plant biotechnology
Ubicación del archivo	http://dx.doi.org/10.1038/s41598-023-47099-4
Categoría OCDE	Ciencia y tecnología: otros temas
Materias	Microbiología aplicada Comunidades microbianas Biotecnología vegetal
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001104793000064
ISSN	2045-2322
Tipo de ruta	verde# dorada
Categoría WOS	Ciencia y tecnología: otros temas
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID 1201386 ANID 1221228 ANID 21210306 ANID 21211278 NSFC 42077386 UFRO DI19-2016 ANID FONDECYT 1201386 ANID FONDECYT 1221228 ANID ICM ICN2021_044