Osteogenic and microstructural characterization in normal versus deformed jaws of rainbow trout (Oncorhynchus mykiss) from freshwater
| Primer Autor |
Salazar, Luis A.
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| Co-autores |
Godoy, Karina
Sandoval, Cristian
Vasquez, Claudio
Manterola-Barroso, Carlos
Toledo, Barbara
Calfuleo, Joel
Beltran, Carolina
Bustamante, Marion
Valderrama, Sebastian
Rojas, Mariana
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| Título |
Osteogenic and microstructural characterization in normal versus deformed jaws of rainbow trout (Oncorhynchus mykiss) from freshwater
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| Editorial |
FRONTIERS MEDIA SA
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| Revista |
FRONTIERS IN MARINE SCIENCE
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| Lenguaje |
en
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| Resumen |
Introduction: During the processes of formation and maturation of farmed salmonids, bone deformities could be associated with changes in the mineralization levels of the axial skeleton and the bone-signaling pathways. Therefore, we aimed to evaluate the gene expression during bone formation and regeneration and their relationship with mineralization in rainbow trout with mandibular deformation. Methods: We included five normal fish and five specimens with mandibular deformation in smolt rainbow trout weighing 400 g and measuring 25 to 35 cm in length. We assessed 1. serum metabolites, 2. microstructure and mandibular bone mineralization and, 3. gene expression of bone signaling pathways. These analyses were done to determine the main causes and/or mechanisms of deformity. Results and discussion: Our results show a marked elevation of bone morphogenetic protein 2 (Bmp2). Also, we found a distinct expression pattern for transcriptional factors, observing diminished RUNX family transcription factor 2 (Runx-2) expression coupled with a simultaneous elevation of osterix (Osx) levels. We also observed decreased osteocalcin and alkaline phosphatase levels related to mineral content loss and an increase in collagen type I as a compensatory structural response. In conclusion, rainbow trout deformation was characterized by demineralization, increased porosity without destruction of the organic matrix, and a moderate decrease in bone mineral content.
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| Fecha Publicación |
2023
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| Tipo de Recurso |
artículo original
|
| doi |
10.3389/fmars.2023.1301449
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| Formato Recurso |
PDF
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| Palabras Claves |
aquaculture
bone
gene expression
microelements
scanning electron microscopy
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| Ubicación del archivo | |
| Categoría OCDE |
Ciencias ambientales y ecología
Biología marina y de agua dulce
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| Materias |
acuicultura
hueso
la expresion genica
microelementos
microscopía electrónica de barrido
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| Identificador del recurso (Mandatado-único) |
artículo original
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| Versión del recurso (Recomendado-único) |
versión publicada
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| License |
CC BY 4.0
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| Condición de la licencia (Recomendado-repetible) |
CC BY 4.0
|
| Derechos de acceso |
acceso abierto
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| Access Rights |
acceso abierto
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| Id de Web of Science |
WOS:001117619200001
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| Tipo de ruta |
verde# dorado
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| Categoría WOS |
Ciencias ambientales y ecología
Biología marina y de agua dulce
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| Referencia del Financiador (Mandatado si es aplicable-repetible) |
UFRO DI20-TD02
|
cienciaabierta.ufro.cl
ciencia.abierta@ufrontera.cl