Repositorio UFRO · Omeka S

Osteogenic and microstructural characterization in normal versus deformed jaws of rainbow trout (Oncorhynchus mykiss) from freshwater

Primer Autor	Salazar, Luis A.
Co-autores	Godoy, Karina Sandoval, Cristian Vasquez, Claudio Manterola-Barroso, Carlos Toledo, Barbara Calfuleo, Joel Beltran, Carolina Bustamante, Marion Valderrama, Sebastian Rojas, Mariana
Título	Osteogenic and microstructural characterization in normal versus deformed jaws of rainbow trout (Oncorhynchus mykiss) from freshwater
Editorial	FRONTIERS MEDIA SA
Revista	FRONTIERS IN MARINE SCIENCE
Lenguaje	en
Resumen	Introduction: During the processes of formation and maturation of farmed salmonids, bone deformities could be associated with changes in the mineralization levels of the axial skeleton and the bone-signaling pathways. Therefore, we aimed to evaluate the gene expression during bone formation and regeneration and their relationship with mineralization in rainbow trout with mandibular deformation. Methods: We included five normal fish and five specimens with mandibular deformation in smolt rainbow trout weighing 400 g and measuring 25 to 35 cm in length. We assessed 1. serum metabolites, 2. microstructure and mandibular bone mineralization and, 3. gene expression of bone signaling pathways. These analyses were done to determine the main causes and/or mechanisms of deformity. Results and discussion: Our results show a marked elevation of bone morphogenetic protein 2 (Bmp2). Also, we found a distinct expression pattern for transcriptional factors, observing diminished RUNX family transcription factor 2 (Runx-2) expression coupled with a simultaneous elevation of osterix (Osx) levels. We also observed decreased osteocalcin and alkaline phosphatase levels related to mineral content loss and an increase in collagen type I as a compensatory structural response. In conclusion, rainbow trout deformation was characterized by demineralization, increased porosity without destruction of the organic matrix, and a moderate decrease in bone mineral content.
Fecha Publicación	2023
Tipo de Recurso	artículo original
doi	10.3389/fmars.2023.1301449
Formato Recurso	PDF
Palabras Claves	aquaculture bone gene expression microelements scanning electron microscopy
Ubicación del archivo	http://dx.doi.org/10.3389/fmars.2023.1301449
Categoría OCDE	Ciencias ambientales y ecología Biología marina y de agua dulce
Materias	acuicultura hueso la expresion genica microelementos microscopía electrónica de barrido
Identificador del recurso (Mandatado-único)	artículo original
Versión del recurso (Recomendado-único)	versión publicada
License	CC BY 4.0
Condición de la licencia (Recomendado-repetible)	CC BY 4.0
Derechos de acceso	acceso abierto
Access Rights	acceso abierto
Id de Web of Science	WOS:001117619200001
Tipo de ruta	verde# dorado
Categoría WOS	Ciencias ambientales y ecología Biología marina y de agua dulce
Referencia del Financiador (Mandatado si es aplicable-repetible)	UFRO DI20-TD02