Repositorio
Institucional

Ulloa, Pilar E.

Pena, Andrea A.

Lizama, Carla D.

Araneda, Cristian

Iturra, Patricia

Neira, Roberto

Medrano, Juan F.

Growth Response and Expression of Muscle Growth-Related Candidate Genes in Adult Zebrafish Fed Plant and Fishmeal Protein-Based Diets

MARY ANN LIEBERT, INC

ZEBRAFISH

en

The main objective of this study was to examine the effects of a plant protein-vs. fishmeal-based diet on growth response in a population of 24 families, as well as expression of growth-related genes in the muscle of adult zebrafish (Danio rerio). Each family was split to create two fish populations with similar genetic backgrounds, and the fish were fed either fishmeal (FM diet) or plant protein (PP diet) as the unique protein source in their diets from 35 to 98 days postfertilization (dpf). To understand the effect of the PP diet on gene expression, individuals from three families, representative of the mean weight in both populations, were selected. To understand the effect of familiar variation on gene expression, the same families were evaluated separately. At 98 dpf, growth-related genes Igf1a, Igf2a, mTOR, Pld1a, Mrf4, Myod, Myogenin, and Myostatin1b were evaluated. In males, Myogenin, Mrf4, and Igf2a showed changes attributable to the PP diet. In females, the effect of the PP diet did not modulate the expression in any of the eight genes studied. The effect of familiar variation on gene expression was observed among families. This study shows that PP diet and family variation have effects on gene expression in fish muscle.

Artículo original

We acknowledge Stephen Gunther for the formulated diets, Alex Cabrera for manufacturing the diets, and Claudio Perez for valuable technical assistance in RT-PCR procedures. We also want to thank Natalia Lam, Jurij Wacyk, and Andrea Bravo for valuable assistance in data and tissue collection. The first author received a doctoral fellowship from the Consorcio Empresarial de Genetica y Desarrollo Biotecnologico para la Industria Salmonera (Aquainnovo S.A). This work was supported by a fellowship of Apoyo a la Realizacion de Tesis Doctoral, from Conicyt AT-24091052 and Aquainnovo S.A. P. E. U. wishes to thank the research group of the Laboratory in Genetic and Biotechnology in Aquaculture at the Universidad de Chile where the fish were reared and the Research Group of the Animal Genomic Laboratory at the University of California Davis.

Agradecemos a Stephen Gunther por las dietas formuladas, a Alex Cabrera por la fabricación de las dietas y a Claudio Pérez por la valiosa asistencia técnica en los procedimientos de RT-PCR. También queremos agradecer a Natalia Lam, Jurij Wacyk y Andrea Bravo por la valiosa asistencia en la recolección de datos y tejidos. El primer autor recibió una beca doctoral del Consorcio Empresarial de Genética y Desarrollo Biotecnológico para la Industria Salmonera (Aquainnovo S.A.). Este trabajo fue apoyado por una beca de Apoyo a la Realización de Tesis Doctoral, de Conicyt AT-24091052 y Aquainnovo S.A. P. E. U. desea agradecer al grupo de investigación del Laboratorio de Genética y Biotecnología en Acuicultura de la Universidad de Chile donde se criaron los peces y al Grupo de Investigación del Laboratorio de Genómica Animal de la Universidad de California Davis.

10.1089/zeb.2012.0823

pdf

TROUT ONCORHYNCHUS-MYKISS

ATLANTIC SALMON

RAINBOW-TROUT

DANIO-RERIO

REPLACEMENT

L.

TRANSCRIPTION

PERFORMANCE

AQUACULTURE

OIL

TRUCHA ONCORHYNCHUS-MYKISS

SALMÓN DEL ATLÁNTICO

TRUCHA ARCOÍRIS

DANIO-RERIO

REEMPLAZO

L.

TRANSCRIPCIÓN

RENDIMIENTO

ACUICULTURA

ACEITE

TROUT ONCORHYNCHUS-MYKISS

ATLANTIC SALMON

RAINBOW-TROUT

DANIO-RERIO

REPLACEMENT

L.

TRANSCRIPTION

PERFORMANCE

AQUACULTURE

OIL

TRUCHA ONCORHYNCHUS-MYKISS

SALMÓN DEL ATLÁNTICO

TRUCHA ARCOÍRIS

DANIO-RERIO

REEMPLAZO

L.

TRANSCRIPCIÓN

RENDIMIENTO

ACUICULTURA

ACEITE

Pesca

Genética y Herencia

Biología Molecular

Growth Response and Expression of Muscle Growth-Related Candidate Genes in Adult Zebrafish Fed Plant and Fishmeal Protein-Based Diets

ZEBRAFISH

hibrida

metadata

PMID 23590402

en

Artículo original

versión publicada

Consorcio Empresarial de Genetica y Desarrollo Biotecnologico para la Industria Salmonera (Aquainnovo S.A)#ANID CONICYT Tesis Doctoral#ANID CONICYT AT-24091052#Aquainnovo S.A.

Consorcio Empresarial de Genetica y Desarrollo Biotecnologico para la Industria Salmonera (Aquainnovo S.A)

ANID CONICYT Tesis Doctoral

ANID CONICYT AT-24091052

Aquainnovo S.A.

MARY ANN LIEBERT, INC

1545-8547

99

109

Biología del desarrollo

Zoología

Developmental Biology

Zoology

pdf

WOS:000317736900011