Repositorio
Institucional

Sangaletti, Naiane

Evangelista Vargas, Shirley

Valdivia Cuya, Martha

Risopatron Gonzalez, Jennie

Sanchez Gutierrez, Raul

Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation

ELSEVIER SCIENCE INC

THERIOGENOLOGY

en

The main objective was to study the effects, on sperm function, of the addition of two superoxide dismutase (SOD) analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation. Twelve alpaca semen samples were collected using an artificial vagina and then diluted at a 1:3 ratio in an extender based on skim milk, egg yolk, and fructose. Each semen sample was divided into three equal parts to form the following groups: control, Tempo (1 mM), and Tempol (1 mM). Groups were cooled to 5 degrees C in 90 minutes (-1 degrees C in 3 minutes); when samples reached approximately 10 degrees C, SOD analogues were added to the respective groups. At 5 degrees C, ethylene glycol (final concentration, 0.1 M) was added to each group. After 30 minutes at 5 degrees C, samples were loaded in 0.25 mL plastic straws, placed in liquid nitrogen vapor for 15 minutes, and then plunged. Percentages of sperm motility, functional sperm membrane integrity, and viable sperm with intact acrosomes were evaluated before and after freeze-thaw using visual analysis, the hypo-osmotic swelling test, and the double-stain trypan blue/giemsa technique, respectively. The Terminal deoxymucleotidyl transferase dUTP Nick End Labeling assay was performed for evaluation of sperm DNA fragmentation of frozen-thawed sperm. Sperm motility was higher (P < 0.05) in the Tempol and Tempo groups than in the control group (mean, 22.1%, 19.7%, and 11.2%, respectively), with similar results for functional sperm membrane integrity. Additionally, DNA fragmentation was lower (P < 0.05) in the Tempol group (16.7%) than in the control group (38.8%). Viable sperm with intact acrosomes were not affected by the use of SOD analogues. There was a negative correlation (r = -0.58) between DNA fragmentation of alpaca sperm and sperm motility after freeze-thawing, but DNA damage was neither related to functional membrane integrity nor viable sperm with intact acrosomes. We concluded that DNA fragmentation and loss of motility during cryopreservation of alpaca sperm could be partially prevented by supplementation of the semen extender with 1 mM Tempo or Tempol. (c) 2013 Elsevier Inc. All rights reserved.

Artículo original

This research was supported by the National Science and Technology Council (CONCYTEC) of Peru, Research Superior Council of San Marcos University (CSI-UNMSM), and Center of Biotechnology in Reproduction (CEBIOR) of La Frontera University. The authors thank Juan Olazabal and other professionals and students of IVITA-Marangani for assistance during collection of alpaca semen samples.

Esta investigación fue financiada por el Consejo Nacional de Ciencia y Tecnología (CONCYTEC) del Perú, el Consejo Superior de Investigaciones Científicas (CSI-UNMSM) de la Universidad de San Marcos y el Centro de Biotecnología en Reproducción (CEBIOR) de la Universidad de La Frontera. Los autores agradecen a Juan Olazábal y a otros profesionales y estudiantes del IVITA-Marangani por su colaboración durante la recolección de muestras de semen de alpaca.

10.1016/j.theriogenology.2012.12.012

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Alpaca sperm

Cryopreservation

Superoxide dismutase

Antioxidant

Camelid

LIPID-PEROXIDATION

SPERM

MOTILITY

SPERMATOZOA

INTEGRITY

TRIS

ANTIOXIDANTS

PARAMETERS

FERTILITY

DILUENTS

Esperma de alpaca

Criopreservación

Superóxido dismutasa

Antioxidante

Camélido

PEROXIDACIÓN LIPÍDICA

ESPERMATOZOIDES

MOTILIDAD

ESPERMATOZOIDES

INTEGRIDAD

TRIS

ANTIOXIDANTES

PARÁMETROS

FERTILIDAD

DILUYENTES

Alpaca sperm

Cryopreservation

Superoxide dismutase

Antioxidant

Camelid

LIPID-PEROXIDATION

SPERM

MOTILITY

SPERMATOZOA

INTEGRITY

TRIS

ANTIOXIDANTS

PARAMETERS

FERTILITY

DILUENTS

Esperma de alpaca

Criopreservación

Superóxido dismutasa

Antioxidante

Camélido

PEROXIDACIÓN LIPÍDICA

ESPERMATOZOIDES

MOTILIDAD

ESPERMATOZOIDES

INTEGRIDAD

TRIS

ANTIOXIDANTES

PARÁMETROS

FERTILIDAD

DILUYENTES

Ciencia Animal y Lechería

Ciencias Veterinarias

Biología Reproductiva

Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation

THERIOGENOLOGY

hibrido

verde

metadato

PMID 23375779

en

Artículo original

versión publicada

National Science and Technology Council (CONCYTEC) of Peru#UNMSM, Research Superior Council (CSI-UNMSM)#UFRO, Center of Biotechnology in Reproduction (CEBIOR)

National Science and Technology Council (CONCYTEC) of Peru

UNMSM, Research Superior Council (CSI-UNMSM)

UFRO, Center of Biotechnology in Reproduction (CEBIOR)

ELSEVIER SCIENCE INC

0093-691X

842

846

Biología reproductiva

Ciencias veterinarias

Reproductive Biology

Veterinary Sciences

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WOS:000315760000015