Repositorio
Institucional

Huygens, D.

Montero Sommerfeld, H.

Diaz, L. M.

Alvarez, M.

Anazco Villanueva, C.

Matus, F.

Boon, N.

Boeckx, P.

High winter diversity of arbuscular mycorrhizal fungal communities in shallow and deep grassland soils

PERGAMON-ELSEVIER SCIENCE LTD

SOIL BIOLOGY & BIOCHEMISTRY

en

The identity and diversity of the arbuscular mycorrhizal fungal (AMF) symbionts have a large impact on ecosystem functioning and stability, indicating the need to assess their distribution in terrestrial environments. Four temperate grassland sites of low to medium fertility located on a common soil were sampled in winter and summer at two different depths (0-40 cm, 40-80 cm) using a spatially intensive experimental sampling design. Arbuscular mycorrhizal fungal community composition was determined via the amplification of AMF rRNA gene fragments present in fine roots combined with terminal-restriction fragment length polymorphism (T-RFLP) and sequencing analyses. For one out of three endonucleases applied, a site x depth interaction on T-RF richness (S) and Shannon's diversity index (H') was observed, indicating differences between sites in the upper soil layer and depth effects on S and H' for the extensively managed, low fertility site. The S and H' of the AMF taxa present in fine plant roots were marginally lower in winter than in summer (-14% and -16% for S and H', respectively). Evenness E did not vary as a function of site, season or depth. The AMF community profiles, as determined by nonmetric multidimensional scaling, differed between sites and seasons, but not among soil depths. The intersite AMF community variations were attributed to niche differentiation based on soil phosphorus availability and pH. Seasonal shifts could not be related to variations in root densities or physicochemical soil properties measured in this study, suggesting climate and plant regulation as major processes responsible for the variations between winter and summer AMF communities. It is concluded that diverse winter AMF communities are present in temperate grassland soils, and that plant roots colonizing subsoils harbour similar and equally diverse AMF assemblages than those present in topsoil layers. (C) 2013 Elsevier Ltd. All rights reserved.

Artículo original

This study was funded by the Chilean National Commission for Scientific and Technological Research (FONDECYT, grant No 1090455) and the Fund for Scientific Research - Flanders (FWO). Presently, D.H. is a postdoctoral fellow funded by the European Commission (International Outgoing Fellowships for career development (IOF) - Marie Curie Actions, PIOF-GA-2011-301443) and the Fund for Scientific Research - Flanders (FWO). Dante Pinochet and Lorena Riedel are acknowledged for their assistance in site selection and soil analysis, and Dr. Manuel Ortiz Labrin of the "Centro de Inseminacion Artificial" of the Universidad Austral de Chile for support during T-RFLP analyses.

Este estudio fue financiado por la Comisión Nacional de Investigación Científica y Tecnológica de Chile (FONDECYT, subvención n.° 1090455) y el Fondo para la Investigación Científica - Flandes (FWO). Actualmente, D.H. es becario postdoctoral financiado por la Comisión Europea (Becas Internacionales para el Desarrollo Profesional (IOF) - Acciones Marie Curie, PIOF-GA-2011-301443) y el Fondo para la Investigación Científica - Flandes (FWO). Se agradece a Dante Pinochet y Lorena Riedel su ayuda en la selección del sitio y el análisis del suelo, y al Dr. Manuel Ortiz Labrin del Centro de Inseminación Artificial de la Universidad Austral de Chile por su apoyo durante los análisis T-RFLP.

10.1016/j.soilbio.2013.06.002

pdf

Arbuscular mycorrhizal fungi

T-RFLP

Grassland

Land use practices

Temporal variations

Subsoil

Deep soil

Chile

Andisol

NITROGEN-FERTILIZATION

SEASONAL-VARIATION

SPECIES RICHNESS

ORGANIC-MATTER

FOREST

BIODIVERSITY

DYNAMICS

ROOTS

MOISTURE

COLONIZATION

Hongos micorrícicos arbusculares

T-RFLP

Pastizales

Prácticas de uso de la tierra

Variaciones temporales

Subsuelo

Suelo profundo

Chile

Andisol

FERTILIZACIÓN CON NITRÓGENO

VARIACIÓN ESTACIONAL

RIQUEZA DE ESPECIES

MATERIA ORGÁNICA

BOSQUE

BIODIVERSIDAD

DINÁMICA

RAÍCES

HUMEDAD

COLONIZACIÓN

Arbuscular mycorrhizal fungi

T-RFLP

Grassland

Land use practices

Temporal variations

Subsoil

Deep soil

Chile

Andisol

NITROGEN-FERTILIZATION

SEASONAL-VARIATION

SPECIES RICHNESS

ORGANIC-MATTER

FOREST

BIODIVERSITY

DYNAMICS

ROOTS

MOISTURE

COLONIZATION

Hongos micorrícicos arbusculares

T-RFLP

Pastizales

Prácticas de uso de la tierra

Variaciones temporales

Subsuelo

Suelo profundo

Chile

Andisol

FERTILIZACIÓN CON NITRÓGENO

VARIACIÓN ESTACIONAL

RIQUEZA DE ESPECIES

MATERIA ORGÁNICA

BOSQUE

BIODIVERSIDAD

DINÁMICA

RAÍCES

HUMEDAD

COLONIZACIÓN

Ciencias del suelo

Ecología

Microbiología

High winter diversity of arbuscular mycorrhizal fungal communities in shallow and deep grassland soils

SOIL BIOLOGY & BIOCHEMISTRY

en

Artículo original

versión publicada

ANID CONICYT FONDECYT 1090455#Fund for Scientific Research - Flanders (FWO)#European Commission PIOF-GA-2011-301443

ANID CONICYT FONDECYT 1090455

Fund for Scientific Research - Flanders (FWO)

European Commission PIOF-GA-2011-301443

PERGAMON-ELSEVIER SCIENCE LTD

0038-0717

236

244

Ciencia del suelo

Soil Science

pdf

WOS:000323686800029