Assessment of Sperm Function Parameters and DNA Fragmentation in Ejaculated Alpaca Sperm (Lama Pacos) by Flow Cytometry

Primer Autor
Risopatron, J.
Co-autores
Cheuqueman, C.
Merino, O.
Giojalas, L.
Baer, AVon
Sanchez, R.
Título
Assessment of Sperm Function Parameters and DNA Fragmentation in Ejaculated Alpaca Sperm (Lama Pacos) by Flow Cytometry
Editorial
WILEY
Revista
REPRODUCTION IN DOMESTIC ANIMALS
Lenguaje
en
Resumen
Contents Flow cytometry has been shown to be an accurate and highly reproducible tool for the analysis of sperm function. The main objective of this study was to assess sperm function parameters in ejaculated alpaca sperm by flow cytometry. Semen samples were collected from six alpaca males and processed for flow cytometric analysis of sperm viability and plasma membrane integrity using SYBR-14/PI staining; acrosomal membrane integrity using FITC-conjugated Pisum Sativum Agglutinin/PI labelling; mitochondrial membrane potential (m) by staining with JC-1 and DNA Fragmentation Index (DFI) by TUNEL. The results indicate that the mean value for sperm viability was 57 +/- 8 %. Spermatozoa with intact acrosome membrane was 87.9 +/- 5%, and viable sperm with intact acrosomal membrane was 46.8 +/- 9%, high mitochondrial membrane potential (m) was detected in 66.32 +/- 9.51% of spermatozoa and mean DFI value was 0.91 +/- 0.9%. The DFI was inversely correlated with high m (p=0.04; r=0.41) and with plasma membrane integrity (p=0.01; r=0.47). To our knowledge, this is the first report of the assessment on the same sample of several parameters of sperm function in ejaculated alpaca sperm by flow cytometry.
Tipo de Recurso
Artículo original
Description
This research was supported by the Universidad de La Frontera, Project No. DI10-2019.
Esta investigación fue financiada por la Universidad de La Frontera, Proyecto No. DI10-2019.
doi
10.1111/rda.12096
Formato Recurso
pdf
Palabras Claves
INTEGRITY
SEMEN
PLASMA
DAMAGE
CRYOPRESERVATION
SPERMATOZOA
INFERTILITY
MOTILITY
QUALITY
STORAGE
INTEGRIDAD
SEMEN
PLASMA
DAÑO
CRIOCONSERVACIÓN
ESPERMATOZOIDES
INFERTILIDAD
MOTILIDAD
CALIDAD
ALMACENAMIENTO
Ubicación del archivo
http://dx.doi.org/10.1111/rda.12096
Materias
INTEGRITY
SEMEN
PLASMA
DAMAGE
CRYOPRESERVATION
SPERMATOZOA
INFERTILITY
MOTILITY
QUALITY
STORAGE
INTEGRIDAD
SEMEN
PLASMA
DAÑO
CRIOCONSERVACIÓN
ESPERMATOZOIDES
INFERTILIDAD
MOTILIDAD
CALIDAD
ALMACENAMIENTO
Disciplinas de la OCDE
Ciencia Animal y lechería
Biología Reproductiva
Ciencias Veterinarias
Título de la cita (Recomendado-único)
Assessment of Sperm Function Parameters and DNA Fragmentation in Ejaculated Alpaca Sperm (Lama Pacos) by Flow Cytometry
Revista/Libro
REPRODUCTION IN DOMESTIC ANIMALS
Tipo de ruta
hibrida
verde
Access Rights
metadata
Derechos de acceso
metadata
Identificador alternativo
PMID 23082871
Idioma
en
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
versión publicada
Entidad financiadora
UFRO DI10-2019
Referencia del Financiador (Mandatado si es aplicable-repetible)
UFRO DI10-2019
Editorial
WILEY
ISSN
0936-6768
Página de inicio (Recomendado-único)
447
Página final (Recomendado-único)
453
Categoría WOS
Agricultura, Lácteos y Ciencia Animal
Biología Reproductiva
Ciencias Veterinarias
Agriculture, Dairy & Animal Science
Reproductive Biology
Veterinary Sciences
Formato
pdf
Id de Web of Science
WOS:000318771000017
Colecciones
Colección Publicaciones Científicas
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