Repositorio UFRO · Omeka S

Differential effects of temperature on reactive oxygen/nitrogen species production in rat pachytene spermatocytes and round spermatids

Primer Autor	Reyes, Juan G.
Co-autores	Pino, Jose A. Osses, Nelson Oyarzun, Daniela Farias, Jorge G. Moreno, Ricardo D.
Título	Differential effects of temperature on reactive oxygen/nitrogen species production in rat pachytene spermatocytes and round spermatids
Editorial	BIOSCIENTIFICA LTD
Revista	REPRODUCTION
Lenguaje	en
Resumen	Reactive oxygen species (ROS) and reactive nitrogen species (RNS) like superoxide and nitric oxide are produced by testis and spermatogenic cells in response to heat stress. However, the magnitude and mechanisms of this production in spermatogenic cells have not been described. In this work, we evaluated ROS/RNS production, its pharmacology, mitochondrial oxidative metabolism, membrane potential and antioxidant capacity at different temperatures in isolated rat pachytene spermatocytes and round spermatids. Our results showed an increment in ROS/RNS production by pachytene spermatocytes when increasing the temperature to 40 degrees C. Instead, ROS/RNS production by round spermatids did not change at temperatures higher than 33 degrees C. ROS/RNS production was sensitive to NADPH oxidase inhibitor diphenylene iodonium or the mitochondrial complex I inhibitor rotenone. No additive effects were observed for these two compounds. Our results suggest an important mitochondrial ROS/RNS production in spermatogenic cells. Oligomycin-insensitive oxygen consumption (uncoupled oxygen consumption) increased with temperature and was significantly larger in round spermatids than pachytene spermatocytes, indicating a likely round spermatid mitochondrial uncoupling at high temperatures. A similar conclusion can be reached by measuring the mitochondrial membrane potential using rhodamine 123 fluorescence in permeabilized cells or JC-1 fluorescence in intact cells. The antioxidant capacity was higher in round spermatids than pachytene spermatocytes at 40 degrees C. Our results strongly suggest that at high temperatures (40 degrees C) pachytene spermatocytes are more susceptible to oxidative stress, but round spermatids are more protected because of a temperature-induced mitochondrial uncoupling together with a larger antioxidant capacity. Reproduction (2013) 145 203-212
Tipo de Recurso	Artículo original
Description	Funding was provided by CONICYT doctoral fellowship to J A Pino (grant numbers 21080629 and 24100103), Fondo Nacional de Ciencia y Tecnologia grant (grant number 1110267) and a PUCV-VRIEA grant to J G Reyes and Fondo Nacional de Ciencia y Tecnologia grant (grant number 1110778) to R D Moreno. Funding was provided by CONICYT doctoral fellowship to J A Pino (grant numbers 21080629 and 24100103), Fondo Nacional de Ciencia y Tecnologia grant (grant number 1110267) and a PUCV-VRIEA grant to J G Reyes and Fondo Nacional de Ciencia y Tecnologia grant (grant number 1110778) to R D Moreno.
doi	10.1530/REP-12-0330
Formato Recurso	pdf
Palabras Claves	GERM-CELL APOPTOSIS SHOCK TRANSCRIPTION FACTOR-1 SPERMATOGENIC CELLS NITRIC-OXIDE HEAT-STRESS INTRACELLULAR CA2+ FUNCTIONAL-ROLE MICE DEPENDENCE TESTIS Apoptosis de células germinales factor de transcripción de choque-1 células espermatogénicas óxido nítrico estrés térmico Ca2+ intracelular función ratones dependencia testículo
Ubicación del archivo	http://dx.doi.org/10.1530/REP-12-0330
Materias	GERM-CELL APOPTOSIS SHOCK TRANSCRIPTION FACTOR-1 SPERMATOGENIC CELLS NITRIC-OXIDE HEAT-STRESS INTRACELLULAR CA2+ FUNCTIONAL-ROLE MICE DEPENDENCE TESTIS Apoptosis de células germinales factor de transcripción de choque-1 células espermatogénicas óxido nítrico estrés térmico Ca2+ intracelular función ratones dependencia testículo
Disciplinas de la OCDE	Biología Reproductiva Fisiología Andrología
Título de la cita (Recomendado-único)	Differential effects of temperature on reactive oxygen/nitrogen species production in rat pachytene spermatocytes and round spermatids
Revista/Libro	REPRODUCTION
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23241345
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT 21080629#ANID CONICYT 24100103#ANID CONICYT FONDECYT 1110267#ANID CONICYT FONDECYT 1110778#PUCV-VRIEA
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT 21080629 ANID CONICYT 24100103 ANID CONICYT FONDECYT 1110267 ANID CONICYT FONDECYT 1110778 PUCV-VRIEA
Editorial	BIOSCIENTIFICA LTD
ISSN	1470-1626
Página de inicio (Recomendado-único)	203
Página final (Recomendado-único)	212
Categoría WOS	Biología del desarrollo Biología reproductiva Developmental Biology Reproductive Biology
Formato	pdf
Id de Web of Science	WOS:000315870800012