Repositorio UFRO · Omeka S

Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome

Primer Autor	Chardot, Thierry
Co-autores	Jolivet, Pascale Acevedo, Francisca Boulard, Celine d'Andrea, Sabine Faure, Jean-Denis Kohli, Ajay Nesi, Nathalie Valot, Benoit
Título	Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome
Editorial	WILEY
Revista	PROTEOMICS
Lenguaje	en
Resumen	Oleaginous seeds store lipids in specialized structures called oil bodies (OBs). These organelles consist of a core of neutral lipids bound by proteins embedded in a phospholipid monolayer. OB proteins are well conserved in plants and have long been grouped into only two categories: structural proteins or enzymes. Recent work, however, which identified other classes of proteins associated with OBs, clearly shows that this classification is obsolete. Proteomics-mediated OB protein identification is facilitated in plants for which the genome is sequenced and annotated. However, it is not clear whether this knowledge can be dependably transposed to less well-characterized plants, including the well-established commercial sources of seed oil as well as the many others being proposed as novel sources for biodiesel, especially in Africa and Asia. Toward an update of the current data available on OB proteins this review discusses (i) the specific difficulties for proteomic studies of organelles; (ii) a 2012 census of the proteins found in seed OBs from various crops; (iii) the oleosin composition of OBs and their role in organelle stability; (iv) PTM of OB proteins as an emerging field of investigation; and finally we describe the emerging model of the OB proteome from oilseed crops.
Tipo de Recurso	Artículo de revisión
Description	This work was partially supported by the "Agence Nationale de la Recherche-The French National Agency" [project ANR-07-GPLA-016] (PJ, CB, SD, NN, TC). F.A. thanks the support provided by PIA Project DI11-7001 from Universidad de La Frontera and Fondecyt project 3120022 from Conicyt. We thank Leigh Gebbie, LKG SCIENTIFIC EDITING & TRANSLATION for her excellent help in correcting the English version of the manuscript. Este trabajo fue financiado parcialmente por la Agencia Nacional de Investigación de Francia (Agence Nationale de la Recherche) [proyecto ANR-07-GPLA-016] (PJ, CB, SD, NN, TC). F.A. agradece el apoyo brindado por el Proyecto DI11-7001 de PIA de la Universidad de La Frontera y el proyecto 3120022 de Fondecyt de Conicyt. Agradecemos a Leigh Gebbie, de LKG SCIENTIFIC EDITING & TRANSLATION, por su excelente ayuda en la corrección de la versión en inglés del manuscrito.
doi	10.1002/pmic.201200431
Formato Recurso	pdf
Palabras Claves	Nonsequenced species Oil bodies Plant proteomics PTM Seeds ARABIDOPSIS-THALIANA OLEOSIN ISOFORMS LIPID DROPLETS YARROWIA-LIPOLYTICA MEMBRANE-PROTEIN N-TERMINI PURIFICATION EXPRESSION CALEOSIN CLONING Especies no secuenciadas Cuerpos lipídicos Proteómica vegetal PTM Semillas ARABIDOPSIS-THALIANA ISOFORMAS DE OLEOSINA GOTAS DE LÍPIDOS YARROWIA-LIPOLYTICA PROTEÍNA DE MEMBRANA N-TERMINALES PURIFICACIÓN EXPRESIÓN CALEOSINA CLONACIÓN
Ubicación del archivo	http://dx.doi.org/10.1002/pmic.201200431
Materias	Nonsequenced species Oil bodies Plant proteomics PTM Seeds ARABIDOPSIS-THALIANA OLEOSIN ISOFORMS LIPID DROPLETS YARROWIA-LIPOLYTICA MEMBRANE-PROTEIN N-TERMINI PURIFICATION EXPRESSION CALEOSIN CLONING Especies no secuenciadas Cuerpos lipídicos Proteómica vegetal PTM Semillas ARABIDOPSIS-THALIANA ISOFORMAS DE OLEOSINA GOTAS DE LÍPIDOS YARROWIA-LIPOLYTICA PROTEÍNA DE MEMBRANA N-TERMINALES PURIFICACIÓN EXPRESIÓN CALEOSINA CLONACIÓN
Disciplinas de la OCDE	Biotecnología Industrial Biotecnología Agrícola Bioquímica
Título de la cita (Recomendado-único)	Crop seed oil bodies: From challenges in protein identification to an emerging picture of the oil body proteome
Revista/Libro	PROTEOMICS
Tipo de ruta	hibrida
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23589365
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo de revisión
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	Agence Nationale de la Recherche-The French National Agency ANR-07-GPLA-016#UFRO PIA DI11-7001#ANID CONICYT FONDECYT 3120022
Referencia del Financiador (Mandatado si es aplicable-repetible)	Agence Nationale de la Recherche-The French National Agency ANR-07-GPLA-016 UFRO PIA DI11-7001 ANID CONICYT FONDECYT 3120022
Editorial	WILEY
ISSN	1615-9853
Página de inicio (Recomendado-único)	1836
Página final (Recomendado-único)	1849
Categoría WOS	Métodos de investigación bioquímica Bioquímica y biología molecular Biochemical Research Methods Biochemistry & Molecular Biology
Formato	pdf
Id de Web of Science	WOS:000327008000006