Repositorio UFRO · Omeka S

Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition

Primer Autor	Saez, Patricia L.
Co-autores	Bravo, Leon A. Latsague, Mirtha I. Toneatti, Marcelo J. Sanchez-Olate, Manuel Rios, Darcy G.
Título	Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition
Editorial	ELSEVIER IRELAND LTD
Revista	PLANT SCIENCE
Lenguaje	en
Resumen	The limited development of photoprotective mechanisms, specifically heat dissipation capacity, found in micropropagated plants may be the result of low xanthophyll cycle pigment content and reduced de-epoxidation capacity making them highly susceptible to photodamage. The effects of gradual or sudden increase of light on Castanea sativa in vitro cultured and during their ex vitro transference was evaluated. The results were compared with those determined in nursery-grown plants. In vitro plants responded poorly to gradual increase in irradiance, exhibiting a low electron transport rate (ETR) agreeing with low non-photochemical quenching (NPQ) and a limited de-epoxidation capacity, not synthesizing detectable amounts of zeaxanthin (Z). Regarding a sudden increase in light (photoinhibition treatment, PhT); post-PhT as in vitro as well nursery plants showed a significant decrease in their maximal efficiency of PSII (F-v/F-m), but in vitro the decrease was very drastic (around 0.2) different from that observed in nursery (around 0.69). In vitro, NPQ was mainly determined by the slow relaxing component, NPQ(s) (80.8%), concomitant with a pronounced decrease of DI protein post-PhT, and a lack of de-epoxidation capacity. During ex vitro transfer, PhT lead to death of some plants, specifically during root induction. The photoprotective mechanisms were activated over time in ex vitro conditions, indicating that micropropagated Castanea sativa display a potential for light acclimation, adjusting their photosynthetic apparatus to the ambient growth irradiance. Understanding the mechanisms that micropropagated plants deployed and how they face high light intensity events, will allow us to search for strategies to improve performance to possible light fluctuations that normally occur in ex vitro conditions during plant acclimation. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
Tipo de Recurso	Artículo original
Description	The authors would like to thank Project INNOVA BIOBIO No. 4 B3-234 and DIUC 210.142.029-1.0 from the Direction of Investigations of the University of Concepcion, for the financial support and Charles L. Guy for review of the manuscript. Patricia L. Saez thanks CONICYT for her doctoral fellowship. Los autores agradecen al Proyecto INNOVA BIOBIO No. 4 B3-234 y a la DIUC 210.142.029-1.0 de la Dirección de Investigaciones de la Universidad de Concepción por el apoyo financiero, y a Charles L. Guy por la revisión del manuscrito. Patricia L. Saez agradece a CONICYT por su beca doctoral.
doi	10.1016/j.plantsci.2012.11.008
Formato Recurso	pdf
Palabras Claves	Photoinhibition Micropropagation Non-photochemical quenching Xanthophylls EX-VITRO CONDITIONS PHOTOSYSTEM-II IN-VITRO XANTHOPHYLL CYCLE EXCESS LIGHT CHLOROPHYLL FLUORESCENCE PHOTOSYNTHETIC CAPACITY ANTENNA SIZE PHOTOPROTECTION DISSIPATION Fotoinhibición Micropropagación Extinción no fotoquímica Xantofilas CONDICIONES EX VITRO FOTOSISTEMA II IN VITRO CICLO DE LA XANTOFILA EXCESO DE LUZ FLUORESCENCIA DE LA CLOROFILA CAPACIDAD FOTOSINTÉTICA TAMAÑO DE LA ANTENA FOTOPROTECCIÓN DISIPACIÓN
Ubicación del archivo	http://dx.doi.org/10.1016/j.plantsci.2012.11.008
Materias	Photoinhibition Micropropagation Non-photochemical quenching Xanthophylls EX-VITRO CONDITIONS PHOTOSYSTEM-II IN-VITRO XANTHOPHYLL CYCLE EXCESS LIGHT CHLOROPHYLL FLUORESCENCE PHOTOSYNTHETIC CAPACITY ANTENNA SIZE PHOTOPROTECTION DISSIPATION Fotoinhibición Micropropagación Extinción no fotoquímica Xantofilas CONDICIONES EX VITRO FOTOSISTEMA II IN VITRO CICLO DE LA XANTOFILA EXCESO DE LUZ FLUORESCENCIA DE LA CLOROFILA CAPACIDAD FOTOSINTÉTICA TAMAÑO DE LA ANTENA FOTOPROTECCIÓN DISIPACIÓN
Disciplinas de la OCDE	Silvicultura Botánica Biotecnología agrícola
Título de la cita (Recomendado-único)	Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition
Revista/Libro	PLANT SCIENCE
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23352399
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	Udec INNOVA BIOBIO 4 B3-234#UdeC DIUC 210.142.029-1.0#ANID CONICYTONICYT
Referencia del Financiador (Mandatado si es aplicable-repetible)	Udec INNOVA BIOBIO 4 B3-234 UdeC DIUC 210.142.029-1.0 ANID CONICYTONICYT
Editorial	ELSEVIER IRELAND LTD
ISSN	0168-9452
Página de inicio (Recomendado-único)	12
Página final (Recomendado-único)	24
Categoría WOS	Bioquímica y biología molecular Ciencias vegetales Biochemistry & Molecular Biology Plant Sciences
Formato	pdf
Id de Web of Science	WOS:000315061800002