Repositorio
Institucional

Ribera-Fonseca, Alejandra

Inostroza-Blancheteau, Claudio

Cartes, Paula

Rengel, Zed

Mora, M. L.

Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

PLANT PHYSIOLOGY AND BIOCHEMISTRY

en

Manganese (Mn) toxicity limits plant growth in acid soils. Although Mn toxicity induces oxidative stress, the role of superoxide dismutase (SOD, EC.1.15.1.1) isoforms in conferring Mn tolerance remains unclear. Seedlings of ryegrass cultivars Nui (Mn-sensitive) and Kingston (Mn-tolerant) were hydroponically grown at 2.4 (optimal) or 750 mu M Mn (toxic) concentration, and harvested from 2 to 48 h. Kingston showed higher shoot Mn than Nui at 2.4;mu M Mn. At toxic supply, shoot Mn concentration steadily increased in both cultivars, with Kingston having the highest accumulation at 48 h. An early (2 h) increase in lipid peroxidation under Mn excess occurred, but it returned (after 6 h) to the basal level in Kingston only. Kingston exhibited higher SOD activity than Nui, and that difference increased due to toxic Mn. In general, Mn-induced gene expression of Mn- and Cu/Zn-SOD isoforms was higher in Nui than Kingston. Nevertheless, under Mn excess, we found a greater Fe-SOD up-regulation (up to 5-fold) in Kingston compared to Nui. Thus, Fe-SOD induction in Kingston might explain, at least partly, its high tolerance to Mn toxicity. This is the first evidence that Mn toxicity causes differential gene expression of SOD isoforms in ryegrass cultivars in the short-term. (C) 2013 Published by Elsevier Masson SAS.

Artículo original

This work was supported by the FONDECYT projects 11100494, 1120901 and 3120248 Grants, CONICYT, Chile.

Este trabajo fue financiado por los proyectos FONDECYT 11100494, 1120901 y 3120248, subvenciones de CONICYT, Chile.

10.1016/j.plaphy.2013.08.012

pdf

Manganese toxicity

Manganese tolerance

Ryegrass

Oxidative stress

Superoxide dismutase isoforms

Fe-SOD

Gene expression

OXIDATIVE STRESS TOLERANCE

QUANTITATIVE RT-PCR

SUPEROXIDE-DISMUTASE

MANGANESE TOXICITY

EXCESS MANGANESE

LOLIUM-PERENNE

PLANTS

TOBACCO

ANTIOXIDANTS

ENHANCEMENT

Toxicidad del manganeso

Tolerancia al manganeso

Raygrass

Estrés oxidativo

Isoformas de la superóxido dismutasa

Fe-SOD

Expresión génica

Tolerancia al estrés oxidativo

RT-PCR cuantitativa

superóxido dismutasa

toxicidad del manganeso

exceso de manganeso

Lolium perenne

plantas

tabaco

antioxidantes

mejora

Manganese toxicity

Manganese tolerance

Ryegrass

Oxidative stress

Superoxide dismutase isoforms

Fe-SOD

Gene expression

OXIDATIVE STRESS TOLERANCE

QUANTITATIVE RT-PCR

SUPEROXIDE-DISMUTASE

MANGANESE TOXICITY

EXCESS MANGANESE

LOLIUM-PERENNE

PLANTS

TOBACCO

ANTIOXIDANTS

ENHANCEMENT

Toxicidad del manganeso

Tolerancia al manganeso

Raygrass

Estrés oxidativo

Isoformas de la superóxido dismutasa

Fe-SOD

Expresión génica

Tolerancia al estrés oxidativo

RT-PCR cuantitativa

superóxido dismutasa

toxicidad del manganeso

exceso de manganeso

Lolium perenne

plantas

tabaco

antioxidantes

mejora

Agronomía

Ciencias del suelo

Biología Molecular

Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass

PLANT PHYSIOLOGY AND BIOCHEMISTRY

hibrida

verde

metadata

metadata

PMID 24077292

en

Artículo original

versión publicada

ANID CONICYT FONDECYT 11100494#ANID CONICYT FONDECYT 1120901#ANID CONICYT FONDECYT 3120248

ANID CONICYT FONDECYT 11100494

ANID CONICYT FONDECYT 1120901

ANID CONICYT FONDECYT 3120248

ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER

0981-9428

77

82

Ciencias de las plantas

Plant Sciences

pdf

WOS:000329007000010