Repositorio UFRO · Omeka S

Molecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry

Primer Autor	Arce-Johnson, P.
Co-autores	Inostroza-Blancheteau, C. Aquea, F. Loyola, R. Slovin, J. Josway, S. Rengel, Z. Reyes-Diaz, M. Alberdi, M.
Título	Molecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry
Editorial	WILEY
Revista	PLANT BIOLOGY
Lenguaje	en
Resumen	Calmodulin (CaM), a small acidic protein, is one of the best characterised Ca2+ sensors in eukaryotes. This Ca2+-regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. Many environmental stresses elicit changes in intracellular Ca2+ activity that could initiate adaptive responses under adverse conditions. We report the first molecular cloning and characterisation of a calmodulin gene, VcCaM1 (Vaccinium corymbosum Calmodulin 1), in the woody shrub, highbush blueberry. VcCaM1 was first identified as VCAL19, a gene induced by aluminium stress in V.corymbosum L. A full-length cDNA of VcCaM1 containing a 766-bp open reading frame (ORF) encoding 149 amino acids was cloned from root RNA. The sequence encodes four Ca2+-binding motifs (EF-hands) and shows high similarity (99%) with the isoform CaM 201 of Daucus carota. Expression analyses showed that following Al treatment, VcCaM1 message level decreased in roots of Brigitta, an Al-resistant cultivar, and after 48h, was lower than in Bluegold, an Al-sensitive cultivar. VcCAM1 message also decreased in leaves of both cultivars within 2h of treatment. Message levels in leaves then increased by 24h to control levels in Brigitta, but not in Bluegold, but then decreased again by 48h. In conclusion, VcCaM1 does not appear to be directly involved in Al resistance, but may be involved in improved plant performance under Al toxicity conditions through regulation of Ca2+ homeostasis and antioxidant systems in leaves.
Tipo de Recurso	Artículo original
Description	This work was financially supported by the Fruit Consortium, 07Genoma01, the Millennium Nucleus for Plant Functional Genomics (P06-009-F), Fondecyt Project No 11080231, and Fondecyt Project No 1080372. Este trabajo fue financiado por Fruit Consortium, 07Genoma01, el Millennium Nucleus for Plant Functional Genomics (P06-009-F), el proyecto Fondecyt n.° 11080231 y el proyecto Fondecyt n.° 1080372.
doi	10.1111/j.1438-8677.2012.00722.x
Formato Recurso	pdf
Palabras Claves	Abiotic stress calcium gene expression qRT-PCR RACE Vaccinium corymbosum SHOCK SIGNAL-TRANSDUCTION CALCIUM BINDING TOXICITY IDENTIFICATION RESISTANCE REGULATOR DYNAMICS ISOFORMS Estrés abiótico calcio expresión génica qRT-PCR RACE Vaccinium corymbosum TRANSDUCCIÓN DE SEÑALES DE CHOQUE CALCIO UNIÓN TOXICIDAD IDENTIFICACIÓN RESISTENCIA REGULADOR DINÁMICA ISOFORMAS
Ubicación del archivo	http://dx.doi.org/10.1111/j.1438-8677.2012.00722.x
Materias	Abiotic stress calcium gene expression qRT-PCR RACE Vaccinium corymbosum SHOCK SIGNAL-TRANSDUCTION CALCIUM BINDING TOXICITY IDENTIFICATION RESISTANCE REGULATOR DYNAMICS ISOFORMS Estrés abiótico calcio expresión génica qRT-PCR RACE Vaccinium corymbosum TRANSDUCCIÓN DE SEÑALES DE CHOQUE CALCIO UNIÓN TOXICIDAD IDENTIFICACIÓN RESISTENCIA REGULADOR DINÁMICA ISOFORMAS
Disciplinas de la OCDE	Biología Molecular Bioquímica Biotecnología agrícola
Título de la cita (Recomendado-único)	Molecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry
Revista/Libro	PLANT BIOLOGY
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23627459
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	Fruit Consortium 07Genoma01#Millennium Nucleus for Plant Functional Genomics P06-009-F#ANID CONICYT FONDECYT 11080231#ANID CONICYT FONDECYT 1080372
Referencia del Financiador (Mandatado si es aplicable-repetible)	Fruit Consortium 07Genoma01 Millennium Nucleus for Plant Functional Genomics P06-009-F ANID CONICYT FONDECYT 11080231 ANID CONICYT FONDECYT 1080372
Editorial	WILEY
ISSN	1435-8603
Página de inicio (Recomendado-único)	1013
Página final (Recomendado-único)	1018
Categoría WOS	Ciencias de las plantas Plant Sciences
Formato	pdf
Id de Web of Science	WOS:000325984000011