Repositorio UFRO · Omeka S

Human choroid plexus papilloma cells efficiently transport glucose and vitamin C

Primer Autor	Nualart, Francisco
Co-autores	Ulloa, Viviana Garcia-Robles, Maria Martinez, Fernando Salazar, Katterine Reinicke, Karin Perez, Fernando Godoy, David F. Godoy, Alejandro S.
Título	Human choroid plexus papilloma cells efficiently transport glucose and vitamin C
Editorial	WILEY
Revista	JOURNAL OF NEUROCHEMISTRY
Lenguaje	en
Resumen	In vitro and in vivo studies suggest that the basolateral membrane of choroid plexus cells, which is in contact with blood vessels, is involved in the uptake of the reduced form of vitamin C, ascorbic acid (AA), through the sodium-vitamin C cotransporter, (SVCT2). Moreover, very low levels of vitamin C were observed in the brains of SVCT2-null mice. The oxidized form of vitamin C, dehydroascorbic acid (DHA), is incorporated through the facilitative glucose transporters (GLUTs). In this study, the contribution of SVCT2 and GLUT1 to vitamin C uptake in human choroid plexus papilloma (HCPP) cells in culture was examined. Both the functional activity and the kinetic parameters of GLUT1 and SVCT2 in cells isolated from HCPP were observed. Finally, DHA uptake by GLUT1 in choroid plexus cells was assessed in the presence of phorbol-12-myristate-13-acetate (PMA)-activated human neutrophils. A marked increase in vitamin C uptake by choroid plexus cells was observed that was associated with superoxide generation and vitamin C oxidation (bystander effect). Thus, vitamin C can be incorporated by epithelial choroid plexus papilloma cells using the basolateral polarization of SVCT2 and GLUT1. This mechanism may be amplified with neutrophil infiltration (inflammation) of choroid plexus tumors.
Tipo de Recurso	Artículo original
Description	The authors thank Ximena Koch from Universidad de Concepcion for her technical support. This study was supported partially by grants from FONDECYT, Chile (1100396) and DIUC from Universidad de Concepcion, Chile. Los autores agradecen a Ximena Koch de la Universidad de Concepción por su apoyo técnico. Este estudio fue financiado parcialmente por subvenciones de FONDECYT, Chile (1100396) y DIUC de la Universidad de Concepción, Chile.
doi	10.1111/jnc.12295
Formato Recurso	pdf
Palabras Claves	brain tumors bystander effect choroid plexus GLUT1 SVCT2 vitamin C BLOOD-BRAIN-BARRIER ASCORBIC-ACID DEHYDROASCORBIC ACID FUNCTIONAL-CHARACTERIZATION GENE LOCALIZATION SKELETAL-MUSCLE GLUT1 EXPRESSION SEQUENCE FAMILY tumores cerebrales efecto espectador plexo coroideo GLUT1 SVCT2 vitamina C BARRERA HEMATOENCEFÁLICA ÁCIDO ASCÓRBICO ÁCIDO DESHIDROASCÓRBICO CARACTERIZACIÓN FUNCIONAL LOCALIZACIÓN GÉNICA MÚSCULO ESQUELÉTICO GLUT1 EXPRESIÓN SECUENCIA FAMILIA
Ubicación del archivo	http://dx.doi.org/10.1111/jnc.12295
Materias	brain tumors bystander effect choroid plexus GLUT1 SVCT2 vitamin C BLOOD-BRAIN-BARRIER ASCORBIC-ACID DEHYDROASCORBIC ACID FUNCTIONAL-CHARACTERIZATION GENE LOCALIZATION SKELETAL-MUSCLE GLUT1 EXPRESSION SEQUENCE FAMILY tumores cerebrales efecto espectador plexo coroideo GLUT1 SVCT2 vitamina C BARRERA HEMATOENCEFÁLICA ÁCIDO ASCÓRBICO ÁCIDO DESHIDROASCÓRBICO CARACTERIZACIÓN FUNCIONAL LOCALIZACIÓN GÉNICA MÚSCULO ESQUELÉTICO GLUT1 EXPRESIÓN SECUENCIA FAMILIA
Disciplinas de la OCDE	Biología Celular Biología Molecular Oncología
Título de la cita (Recomendado-único)	Human choroid plexus papilloma cells efficiently transport glucose and vitamin C
Revista/Libro	JOURNAL OF NEUROCHEMISTRY
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23647458
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT FONDECYT 1100396#UC DI
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT FONDECYT 1100396 UC DI
Editorial	WILEY
ISSN	0022-3042
Página de inicio (Recomendado-único)	403
Página final (Recomendado-único)	414
Categoría WOS	Bioquímica y biología molecular Neurociencias Biochemistry & Molecular Biology Neurosciences
Formato	pdf
Id de Web of Science	WOS:000328467000006