Biochar Derived from Agricultural and Forestry Residual Biomass: Characterization and Potential Application for Enzymes Immobilization

Primer Autor
Cea, M.
Co-autores
Gonzalez, M. E.
Sangaletti, N.
Gonzalez, A.
Toro, C.
Diez, M. C.
Moreno, N.
Querol, X.
Navia, R.
Título
Biochar Derived from Agricultural and Forestry Residual Biomass: Characterization and Potential Application for Enzymes Immobilization
Editorial
AMER SCIENTIFIC PUBLISHERS
Revista
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
Lenguaje
en
Resumen
Very much attention has been focused on lipases as these enzymes can be used as biocatalysts, allowing a cost effective and environmentally friendly method to efficiently catalyze specific reactions. However, its application at industrial scale is still limited due to several shortcomings including low stability in their native state, inhibition by organic solvents and exhaustion of enzyme activity. To overcome these problems, lipases have been immobilized by several methods onto various supports. In this context, biochar, a low-cost material derived from the pyrolysis of residual biomass, constitutes a promising immobilization support material for enzymes due its suitable physicochemical and structural properties. In this study a complete physico-chemical and mineralogical characterization of biochar derived from pyrolyzed oat hull and pine bark at 300 and 500 degrees C is presented. In addition, a preliminary study on the immobilization of Candida rugosa lipase using biochar derived from oat hull pyrolyzed at 300 degrees C is reported. The results obtained showed that the structural and chemical properties of biochar depend on the raw materials used and pyrolysis temperature. The specific surface area (BET) presented a similar trend, increasing with an increase in pyrolysis temperature. High enrichment of trace elements such as Ba, Cr, Cu, V and Zn was detected in biochar from pine bark and was discarded for lipase immobilization purposes. The binding efficiency of lipase was in the range of 40-60%, depending on biochar particle size. The higher enzymatic activity yields were associated to small particle size of oat hull biochar. However, a reduction in Candida rugosa lipase activity yield compared with the free enzyme was obtained.
Tipo de Recurso
Artículo original
Description
This work was supported by CONICYT-Chile FONDEF Project D0711096, CONICYT project No 79090009 and Fondecyt No 11110388. Furthermore, authors thank Scholarship "Becas Chile" from CONICYT. Authors also acknowledge the bilateral projects 2009-145 CONICYT/CSIC and 2009CL0062 CSIC/CONICYT between University of La Frontera and IDAEA-CSIC.
Este trabajo fue financiado por el proyecto D0711096 de CONICYT-Chile FONDEF, el proyecto N.° 79090009 de CONICYT y el Fondecyt N.° 11110388. Asimismo, los autores agradecen la beca "Becas Chile" otorgada por CONICYT. Los autores también reconocen los proyectos bilaterales 2009-145 CONICYT/CSIC y 2009CL0062 CSIC/CONICYT entre la Universidad de La Frontera e IDAEA-CSIC.
doi
10.1166/jbmb.2013.1373
Formato Recurso
pdf
Palabras Claves
Agro-Forestry Residues
Pyrolysis
Biochar
Characterization
Lipase
Immobilization
LIPASE
PYROLYSIS
PURIFICATION
CHARS
PLANT
PINE
Residuos agroforestales
Pirólisis
Biocarbón
Caracterización
Lipasa
Inmovilización
LIPASA
PIRÓLISIS
PURIFICACIÓN
CARBÓN
PLANTA
PINO
Ubicación del archivo
http://dx.doi.org/10.1166/jbmb.2013.1373
Materias
Agro-Forestry Residues
Pyrolysis
Biochar
Characterization
Lipase
Immobilization
LIPASE
PYROLYSIS
PURIFICATION
CHARS
PLANT
PINE
Residuos agroforestales
Pirólisis
Biocarbón
Caracterización
Lipasa
Inmovilización
LIPASA
PIRÓLISIS
PURIFICACIÓN
CARBÓN
PLANTA
PINO
Disciplinas de la OCDE
Biotecnología Industrial
Tecnología de Bioprocesamiento, Biocatálisis y Fermentación
Bioquímica
Título de la cita (Recomendado-único)
Biochar Derived from Agricultural and Forestry Residual Biomass: Characterization and Potential Application for Enzymes Immobilization
Revista/Libro
JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
Tipo de ruta
hibrida
verde
Access Rights
metadata
Derechos de acceso
metadata
Idioma
en
Identificador del recurso (Mandatado-único)
Artículo original
Versión del recurso (Recomendado-único)
versión publicada
Entidad financiadora
ANID CONICYT FONDEF D0711096#ANID CONICYT 79090009#ANID CONICYT FONDECYT 11110388#ANID CONICYT/CSIC 2009-145#ANID CSIC/CONICYT UFRO e IDAEA-CSIC 2009CL0062
Referencia del Financiador (Mandatado si es aplicable-repetible)
ANID CONICYT FONDEF D0711096
ANID CONICYT 79090009
ANID CONICYT FONDECYT 11110388
ANID CONICYT/CSIC 2009-145
ANID CSIC/CONICYT UFRO e IDAEA-CSIC 2009CL0062
Editorial
AMER SCIENTIFIC PUBLISHERS
ISSN
1556-6560
Página de inicio (Recomendado-único)
724
Página final (Recomendado-único)
732
Categoría WOS
Química Aplicada
Energía y Combustibles
Ciencia de los Materiales, Biomateriales
Chemistry, Applied
Energy & Fuels
Materials Science, Biomaterials
Formato
pdf
Id de Web of Science
WOS:000327166900010
Colecciones
Colección Publicaciones Científicas
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