Repositorio UFRO · Omeka S

Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function

Primer Autor	Paredes, Marco
Co-autores	Gonzalez, Katerina Figueroa, Jaime Montiel-Eulefi, Enrique
Título	Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function
Editorial	SPRINGER
Revista	FISH PHYSIOLOGY AND BIOCHEMISTRY
Lenguaje	en
Resumen	The in vitro and in vivo effect of prolactin (PRL) on kidney macrophages from Atlantic salmon (Salmo salar) was investigated under the assumption that PRL stimulates immune innate response in mammals. Kidney macrophages were treated two ways: first, cultured in RPMI 1640 medium containing 10, 25, 50 and 100 ng/mL of PRL and second, isolated from a fish with a PRL-injected dose of 100 ng/Kg. Reduced nitro blue tetrazolium (formazan) was used to produce intracellular superoxide anion. Phagocytic activity of PRL was determined in treated cells by optical microscopy observation of phagocytized Congo red-stained yeast. Kidney lysozyme activity was measured in PRL-injected fish. In vitro and in vivo macrophages treated with PRL presented an enhanced superoxide anion production, elevated phagocytic index and increased phagocytic activity. Treated fish showed higher levels of lysozyme activity in the head kidney compared to the control. These results indicate that PRL-stimulated innate immune response in Atlantic salmon and future studies will allow us to assess the possibility of using PRL as an immunostimulant in the Chilean salmon industry.
Tipo de Recurso	Artículo original
Description	This work is supported by FONDECYT grant 1040073 and DID-Doctorado grant 2005-07 from the Universidad Austral de Chile. Este trabajo cuenta con el apoyo de la beca FONDECYT 1040073 y la beca DID-Doctorado 2005-07 de la Universidad Austral de Chile.
doi	10.1007/s10695-013-9777-7
Formato Recurso	pdf
Palabras Claves	Salmo salar Prolactin Phagocytic activity Superoxide anion FISH GILTHEAD SEABREAM GROWTH-HORMONE DEFENSE-MECHANISMS FEED SUPPLEMENT RAINBOW-TROUT NADPH OXIDASE BETA-GLUCAN ACTIVATION CELLS ANTIBIOTICS Salmo salar Prolactina Actividad fagocítica Anión superóxido DORADA HORMONA DE CRECIMIENTO MECANISMOS DE DEFENSA SUPLEMENTO ALIMENTICIO TRUCHA ARCOÍRIS NADPH OXIDASA BETA-GLUCANO ACTIVACIÓN CÉLULAS ANTIBIÓTICOS
Ubicación del archivo	http://dx.doi.org/10.1007/s10695-013-9777-7
Materias	Salmo salar Prolactin Phagocytic activity Superoxide anion FISH GILTHEAD SEABREAM GROWTH-HORMONE DEFENSE-MECHANISMS FEED SUPPLEMENT RAINBOW-TROUT NADPH OXIDASE BETA-GLUCAN ACTIVATION CELLS ANTIBIOTICS Salmo salar Prolactina Actividad fagocítica Anión superóxido DORADA HORMONA DE CRECIMIENTO MECANISMOS DE DEFENSA SUPLEMENTO ALIMENTICIO TRUCHA ARCOÍRIS NADPH OXIDASA BETA-GLUCANO ACTIVACIÓN CÉLULAS ANTIBIÓTICOS
Disciplinas de la OCDE	Pesca Ciencias Veterinarias Inmunología
Título de la cita (Recomendado-único)	Immunomodulatory effect of prolactin on Atlantic salmon (Salmo salar) macrophage function
Revista/Libro	FISH PHYSIOLOGY AND BIOCHEMISTRY
Tipo de ruta	hibrida verde
Access Rights	restringido
Derechos de acceso	restringido
Identificador alternativo	PMID 23420569
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT FONDECYT 1040073 Universidad Austral de Chile
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT FONDECYT 1040073 Universidad Austral de Chile
Editorial	SPRINGER
ISSN	0920-1742
Página de inicio (Recomendado-único)	1215
Página final (Recomendado-único)	1221
Categoría WOS	Bioquímica y biología molecular Pesca Fisiología Biochemistry & Molecular Biology Fisheries Physiology
Formato	pdf
Id de Web of Science	WOS:000324339500014