Repositorio UFRO · Omeka S

Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere

Primer Autor	Jorquera, Milko A.
Co-autores	Saavedra, Nicolas Maruyama, Fumito Richardson, Alan E. Crowley, David E. Catrilaf, Rosa del C. Henriquez, Evelyn J. de la Luz Mora, Maria
Título	Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere
Editorial	OXFORD UNIV PRESS
Revista	FEMS MICROBIOLOGY ECOLOGY
Lenguaje	en
Resumen	Phytate-mineralizing rhizobacteria (PMR) perform an essential function for the mineralization of organic phosphorus but little is known about their ecology in soils and rhizosphere. In this study, PCR-based methods were developed for detection and quantification of the Bacillus beta-propeller phytase (BPP) gene. Experiments were conducted to monitor the presence and persistence of a phytate-mineralizing strain, Bacillus sp. MQH19, after inoculation of soil microcosms and within the rhizosphere. The occurrence of the BPP gene in natural pasture soils from Chilean Andisols was also examined. The results showed that the Bacillus BPP gene was readily detected in sterile and nonsterile microcosms, and that the quantitative PCR (qPCR) methods could be used to monitor changes in the abundance of the BPP gene over time. Our results also show that the addition of phytate to nonsterile soils induced the expression of the BPP gene in the rhizosphere of ryegrass and the BPP gene was detected in all pasture soils sampled. This study shows that phytate addition soils induced changes in the abundance and expression of Bacillus BPP to genes in the rhizosphere and demonstrates that Bacillus BPP gene is cosmopolitan in pasture soils from Chilean Andisols.
Tipo de Recurso	Artículo original
Description	This study was supported by FONDECYT Initiation no. 11080159, FONDECYT Regular no. 1100625 and MEC-CONICYT no. 80110001. The authors acknowledge the suggestions made by the Editor and anonymous reviewers to improve the quality of this manuscript. Este estudio fue financiado por FONDECYT (Iniciación n.° 11080159), FONDECYT (Regular n.° 1100625) y MEC-CONICYT (n.° 80110001). Los autores agradecen las sugerencias del editor y de los revisores anónimos para mejorar la calidad de este manuscrito.
doi	10.1111/j.1574-6941.2012.01480.x
Formato Recurso	pdf
Palabras Claves	phytase phytate rhizobacteria rhizosphere soil DIVERSITY BACTERIA fitasa fitato rizobacterias rizosfera suelo DIVERSIDAD BACTERIAS
Ubicación del archivo	http://dx.doi.org/10.1111/j.1574-6941.2012.01480.x
Materias	phytase phytate rhizobacteria rhizosphere soil DIVERSITY BACTERIA fitasa fitato rizobacterias rizosfera suelo DIVERSIDAD BACTERIAS
Disciplinas de la OCDE	Biotecnología agrícola Ciencias del suelo Microbiología
Título de la cita (Recomendado-único)	Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere
Revista/Libro	FEMS MICROBIOLOGY ECOLOGY
Tipo de ruta	hibrida verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
Identificador alternativo	PMID 22928980
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT FONDECYT 11080159#ANID CONICYT FONDECYT 1100625 ANID CONICYT MEC-CONICYT 80110001
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT FONDECYT 11080159 ANID CONICYT FONDECYT 1100625 ANID CONICYT MEC-CONICYT 80110001
Editorial	OXFORD UNIV PRESS
ISSN	0168-6496
Página de inicio (Recomendado-único)	352
Página final (Recomendado-único)	360
Categoría WOS	Microbiología Microbiology
Formato	pdf
Id de Web of Science	WOS:000313252600008