Repositorio UFRO · Omeka S

Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain

Primer Autor	Shene, Carolina
Co-autores	Leyton, Allison Rubilar, Monica Pinelo, Manuel Acevedo, Francisca Morales, Eduardo
Título	Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain
Editorial	WILEY
Revista	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Lenguaje	en
Resumen	Production of docosahexasaenoic acid (DHA) by a native Labyrinthulomycetes strain, Thraustochytriidae sp. TN5, whose growth characteristics present some differences to related strains, was scaled from shaken flask to a laboratory fermentor. The effect of the growth medium composition and growth conditions for (i) biomass production, (ii) lipid content of the biomass, and (iii) DHA content in the lipids was determined. Taguchi's design of experiments was used to study the influence of two discrete - carbon and nitrogen sources - and six continuous - concentrations of the carbon and nitrogen sources, yeast extract and artificial seawater, incubation temperature, and time- factors on the three mentioned variables. In the flask experiments the lipidic content in the biomass (25.2% w/w) and DHA concentration (0.48g/L) were the highest at the following conditions: maltose 20g/L, sodium glutamate 2.4g/L, yeast extract 6g/L, 72h, 25 degrees C, and artificial seawater 30% v/v; under these conditions biomass concentration was 5.1g/L. Fed-batch allowed to increase biomass concentration to 14g/L. The lipidic fraction of Thraustochytriidae sp. TN5 biomass in the repeated batches was found between 16.2 and 34.8% w/w. Under this growth condition lipids and DHA productivities were 50 and 23mg/(Lh), respectively. Practical applications: Consumption of long chain-polyunsaturated fatty acids (LC-PUFA) belonging to the omega-3 family such as DHA has several positive effects on human and animal health. However, natural sources are restricted to cold-water fish and their oils. Marine protists are also good candidates for the production of microbial DHA. For evaluating the potential of new strains the effect of the growth medium composition, growth conditions, and cultivation mode on DHA productivity has to be determined. In this work the production of DHA by a native Labyrinthulomycetes strain was scaled from shaken flask to a laboratory fermentor with the aid of Taguchi's methodology, process in which biomass concentration, lipid content in the biomass, and DHA content in the lipids were increased.
Tipo de Recurso	Artículo original
Description	The authors thank Direccion de Investigacion at Universidad de La Frontera for the economic support provided through Convenios de Desempeno I, II, and GAP. Los autores agradecen a la Dirección de Investigación de la Universidad de La Frontera por el apoyo económico brindado a través de los Convenios de Desempeño I, II y GAP.
doi	10.1002/ejlt.201200417
Formato Recurso	pdf
Palabras Claves	Fed-batch fermentation Lipidic biomass Polyunsaturated fatty acids Taguchi's orthogonal arrays Thraustochytrids AUREUM ATCC 34304 POLYUNSATURATED FATTY-ACIDS DOCOSAHEXAENOIC ACID SCHIZOCHYTRIUM-MANGROVEI EXTRACTION METHODS HIGH YIELDS CULTURE OPTIMIZATION GROWTH ACCUMULATION Fermentación por lotes alimentados Biomasa lipídica Ácidos grasos poliinsaturados Matrices ortogonales de Taguchi Traustocítridos AUREUM ATCC 34304 ÁCIDOS GRASOS POLIINSATURADOS ÁCIDO DOCOSAHEXAENOICO SCHIZOCHYTRIUM-MANGROVEI MÉTODOS DE EXTRACCIÓN ALTOS RENDIMIENTOS CULTIVO OPTIMIZACIÓN CRECIMIENTO ACUMULACIÓN
Ubicación del archivo	http://dx.doi.org/10.1002/ejlt.201200417
Materias	Fed-batch fermentation Lipidic biomass Polyunsaturated fatty acids Taguchi's orthogonal arrays Thraustochytrids AUREUM ATCC 34304 POLYUNSATURATED FATTY-ACIDS DOCOSAHEXAENOIC ACID SCHIZOCHYTRIUM-MANGROVEI EXTRACTION METHODS HIGH YIELDS CULTURE OPTIMIZATION GROWTH ACCUMULATION Fermentación por lotes alimentados Biomasa lipídica Ácidos grasos poliinsaturados Matrices ortogonales de Taguchi Traustocítridos AUREUM ATCC 34304 ÁCIDOS GRASOS POLIINSATURADOS ÁCIDO DOCOSAHEXAENOICO SCHIZOCHYTRIUM-MANGROVEI MÉTODOS DE EXTRACCIÓN ALTOS RENDIMIENTOS CULTIVO OPTIMIZACIÓN CRECIMIENTO ACUMULACIÓN
Disciplinas de la OCDE	Tecnología de los Alimentos Biotecnología Alimentaria Nutrición y Dietética
Título de la cita (Recomendado-único)	Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain
Revista/Libro	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	UFRO DI
Referencia del Financiador (Mandatado si es aplicable-repetible)	UFRO DI
Editorial	WILEY
ISSN	1438-7697
Página de inicio (Recomendado-único)	890
Página final (Recomendado-único)	900
Categoría WOS	Ciencia y tecnología de los alimentos Nutrición y dietética Food Science & Technology Nutrition & Dietetics
Formato	pdf
Id de Web of Science	WOS:000327815500007