Repositorio UFRO · Omeka S

Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles

Primer Autor	Greiner, Ralf
Co-autores	Konietzny, Ursula Blackburn, Daniel Menezes Jorquera, Milko A.
Título	Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles
Editorial	ELSEVIER SCI LTD
Revista	BIORESOURCE TECHNOLOGY
Lenguaje	en
Resumen	Phytases of different origin were covalently bound onto Fe3O4 magnetic nanoparticles (12 nm). Binding efficiencies of all three phytases were well above 70% relative to the number of aldehyde groups available on the surface of the magnetic nanoparticles. Temperature stability for all three phytases was enhanced as a consequence of immobilisation, whereas pH dependence of enzyme activity was not affected. Maximum catalytic activity of the immobilised phytases was found at 60 degrees C (rye), 65 degrees C (Aspergillus niger) and 70 degrees C (Escherichia albertii). The immobilised enzymes exhibited the same excellent substrate specificities and unique myo-inositol phosphate phosphatase activities as their soluble counterparts. However, the catalytic turnover number dropped drastically for the immobilised phytases. The amount of the desired partially phosphorylated myo-inositol phosphate isomer could be easily controlled by the contact time of substrate solution and immobilised enzymes. The immobilised phytases showed a high operational stability by retaining almost full activity even after fifty uses. (C) 2013 Elsevier Ltd. All rights reserved.
Tipo de Recurso	Artículo original
Description	This work was supported by the Co-Operative Project "Biotechnological Application of free and immobilised Phytases" funded by the German Federal Ministry for Education and Research (BMBF) and Comision Nacional de Investigacion Cientifica y Tecnologica de Chile (CONICYT), CHL 09/009. Este trabajo fue financiado por el Proyecto Cooperativo "Aplicación Biotecnológica de Fitasas libres e inmovilizadas", financiado por el Ministerio Federal Alemán de Educación e Investigación (BMBF) y la Comisión Nacional de Investigación Científica y Tecnológica de Chile (CONICYT), CHL 09/009.
doi	10.1016/j.biortech.2013.05.056
Formato Recurso	pdf
Palabras Claves	Immobilisation Magnetic nanoparticles myo-inositol phosphates Phytate Phytase PURIFICATION ISOMERS ENZYMES Inmovilización Nanopartículas magnéticas Mioinositol fosfatos Fitato Fitasa PURIFICACIÓN ISÓMEROS ENZIMAS
Ubicación del archivo	http://dx.doi.org/10.1016/j.biortech.2013.05.056
Materias	Immobilisation Magnetic nanoparticles myo-inositol phosphates Phytate Phytase PURIFICATION ISOMERS ENZYMES Inmovilización Nanopartículas magnéticas Mioinositol fosfatos Fitato Fitasa PURIFICACIÓN ISÓMEROS ENZIMAS
Disciplinas de la OCDE	Biotecnología Industrial Tecnología de Bioprocesamiento, Biocatálisis y Fermentación Bioquímica
Título de la cita (Recomendado-único)	Production of partially phosphorylated myo-inositol phosphates using phytases immobilised on magnetic nanoparticles
Revista/Libro	BIORESOURCE TECHNOLOGY
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Identificador alternativo	PMID 23747448
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	Co-Operative Project "Biotechnological Application of free and immobilised Phytases"#German Federal Ministry for Education and Research (BMBF)#ANID CONICYT CHL 09/009
Referencia del Financiador (Mandatado si es aplicable-repetible)	Co-Operative Project "Biotechnological Application of free and immobilised Phytases" German Federal Ministry for Education and Research (BMBF) ANID CONICYT CHL 09/009
Editorial	ELSEVIER SCI LTD
ISSN	0960-8524
Página de inicio (Recomendado-único)	375
Página final (Recomendado-único)	383
Categoría WOS	Ingeniería Agrícola Biotecnología y Microbiología Aplicada Energía y Combustibles Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels
Formato	pdf
Id de Web of Science	WOS:000322292800050