Repositorio UFRO · Omeka S

Selenobacteria selected from the rhizosphere as a potential tool for Se biofortification of wheat crops

Primer Autor	de la Luz Mora, Maria
Co-autores	Acuna, Jacquelinne J. Jorquera, Milko A. Barra, Patricio J. Crowley, David E.
Título	Selenobacteria selected from the rhizosphere as a potential tool for Se biofortification of wheat crops
Editorial	SPRINGER
Revista	BIOLOGY AND FERTILITY OF SOILS
Lenguaje	en
Resumen	Cereal production in southern Chile is based on ash-derived volcanic Andisols, which present suboptimal levels of available selenium (Se). Strategies are needed to improve Se content in cereal crops and concomitantly improve the nutritional quality of grain. Here, we investigated the occurrence of Se-tolerant bacteria (STB) in Andisols and evaluated Se tolerance and accumulation in STB. The inoculation of wheat with STB and the contributions of these bacteria to Se content in plants were also evaluated under greenhouse conditions. The results showed that Se amendment of Andisols stimulated some bacterial groups (Paenibacillaceae and Brucellaceae) but inhibited others (Clostridia, Burkholderiales, Chitinophagaceae and Oxalobacteraceae), as revealed by denaturing gradient gel electrophoresis. Furthermore, we found four STB isolates that displayed 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase activity) and that carried the acdS gene as revealed by PCR. The selected STB were characterised as Stenotrophomonas, Bacillus, Enterobacter and Pseudomonas according to partial sequencing of the 16S rRNA gene. After 24 h of culture in nutrient broth, the selected STB showed the ability to grow in high Se concentrations (5 and 10 mM) and to accumulate elemental Se in micro- and nanospherical deposits, transforming 50-80 % of the Se initially added. Greenhouse experiments with wheat showed that Se associated with STB (micro- and nanospheres of elemental Se and other intracellular forms) can be translocated into leaves of wheat plantlets.
Tipo de Recurso	Artículo original
Description	This study was financed by FONDECYT No. 1100625. Jacquelinne Acuna thanks the CONICYT Ph.D. scholarships. Este estudio fue financiado por FONDECYT No. 1100625. Jacquelinne Acuña agradece las becas de doctorado de CONICYT.
doi	10.1007/s00374-012-0705-2
Formato Recurso	pdf
Palabras Claves	Biofortification Cereals Rhizobacteria Selenium Andisol BACTERIAL COMMUNITY COMPOSITION GROWTH-PROMOTING RHIZOBACTERIA PLANT-GROWTH BRASSICA-NAPUS ACC DEAMINASE SELENIUM REDUCTION SELENATE STRAIN SOIL Biofortificación Cereales Rizobacterias Selenio Andisol COMPOSICIÓN DE LA COMUNIDAD BACTERIANA RIZOBACTERIIAS PROMOTORAS DEL CRECIMIENTO CRECIMIENTO VEGETAL BRASSICA NAPUS ACC DEAMINASA SELENIO REDUCCIÓN SELENATO CEPA SUELO
Ubicación del archivo	http://dx.doi.org/10.1007/s00374-012-0705-2
Materias	Biofortification Cereals Rhizobacteria Selenium Andisol BACTERIAL COMMUNITY COMPOSITION GROWTH-PROMOTING RHIZOBACTERIA PLANT-GROWTH BRASSICA-NAPUS ACC DEAMINASE SELENIUM REDUCTION SELENATE STRAIN SOIL Biofortificación Cereales Rizobacterias Selenio Andisol COMPOSICIÓN DE LA COMUNIDAD BACTERIANA RIZOBACTERIIAS PROMOTORAS DEL CRECIMIENTO CRECIMIENTO VEGETAL BRASSICA NAPUS ACC DEAMINASA SELENIO REDUCCIÓN SELENATO CEPA SUELO
Disciplinas de la OCDE	Biotecnología agrícola Ciencias del suelo Microbiología
Título de la cita (Recomendado-único)	Selenobacteria selected from the rhizosphere as a potential tool for Se biofortification of wheat crops
Revista/Libro	BIOLOGY AND FERTILITY OF SOILS
Tipo de ruta	hibrida verde
Access Rights	restringido
Derechos de acceso	restringido
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT FONDECYT 1100625
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT FONDECYT 1100625
Editorial	SPRINGER
ISSN	0178-2762
Página de inicio (Recomendado-único)	175
Página final (Recomendado-único)	185
Categoría WOS	Ciencia del suelo Soil Science
Formato	pdf
Id de Web of Science	WOS:000314064300006