Repositorio UFRO · Omeka S

Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters

Primer Autor	Figueroa, E.
Co-autores	Risopatron, J. Sanchez, R. Isachenko, E. Merino, O. Isachenko, V. Valdebenito, I.
Título	Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Editorial	ELSEVIER
Revista	AQUACULTURE
Lenguaje	en
Resumen	This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a standard buffer for fish spermatozoa (Cortland (R) medium) + 10% DMSO + 2% BSA + 0.13 M sucrose + SP at concentrations of 30% (group 1), 40% (group 2) or 50% (group 3). Fresh sperm (F) was used as a control. For cooling, 30 mu L suspensions of spermatozoa from each group were dropped directly into liquid nitrogen. After cooling, the spheres were placed in cryotubes for storage in liquid nitrogen. For thawing, cryotubes with the vitrified spermatozoa were placed in a water bath at 37 degrees C for 60 s. After warming, the following sperm quality parameters were determined by flow cytometry: viability and plasma membrane integrity (SYBR-14/PI, staining technique); mitochondrial membrane integrity (JC-1 staining); and DNA fragmentation (TUNEL). Sperm function was assessed by fertility trials. Spermatozoa quality variables were best preserved when the highest SP concentration (50%) was used (viability 97.3%, plasma membrane integrity 98.4%, mitochondrial membrane integrity 36.2%, DNA fragmentation 11.1%). Similarly, fertilization rates were higher with sperm that were vitrified in medium with 50% SP. This is the first report on the physiological parameters of the cryopreservation of spermatozoa from sex-reversed rainbow trout (Oncorhynchus mykiss) by direct plunging into liquid nitrogen (vitrification). (C) 2012 Elsevier B. V. All rights reserved.
Tipo de Recurso	Artículo original
Description	This work was supported by Fund for the Promotion of Scientific and Technological Development FONDEF (IDO6I1020-D10I1064) Temuco, Chile and the company Troutlodge Chile for providing the gametes used in the biotests. Este trabajo fue financiado por el Fondo para el Fomento del Desarrollo Científico y Tecnológico (FONDEF, IDO6I1020-D10I1064) de Temuco, Chile, y por la empresa Troutlodge Chile, que proporcionó los gametos utilizados en las pruebas biológicas.
doi	10.1016/j.aquaculture.2012.10.019
Formato Recurso	pdf
Palabras Claves	Spermatozoa vitrification Sex-reversed Oncorhynchus mykiss Seminal plasma Spermatozoa function COLD-SHOCK DAMAGE SPERM CRYOPRESERVATION DNA INTEGRITY OXIDATIVE STRESS SPARUS-AURATA MOTILITY CRYOPROTECTANT PROTEINS VIABILITY QUALITY Vitrificación de espermatozoides Oncorhynchus mykiss con inversión sexual Plasma seminal Función de los espermatozoides DAÑO POR CHOQUE POR FRÍO CRIOCONSERVACIÓN DE ESPERMA INTEGRIDAD DEL ADN ESTRÉS OXIDATIVO SPARUS AURATA MOTILIDAD CRIOPROTECTOR PROTEÍNAS VIABILIDAD CALIDAD
Ubicación del archivo	http://dx.doi.org/10.1016/j.aquaculture.2012.10.019
Materias	Spermatozoa vitrification Sex-reversed Oncorhynchus mykiss Seminal plasma Spermatozoa function COLD-SHOCK DAMAGE SPERM CRYOPRESERVATION DNA INTEGRITY OXIDATIVE STRESS SPARUS-AURATA MOTILITY CRYOPROTECTANT PROTEINS VIABILITY QUALITY Vitrificación de espermatozoides Oncorhynchus mykiss con inversión sexual Plasma seminal Función de los espermatozoides DAÑO POR CHOQUE POR FRÍO CRIOCONSERVACIÓN DE ESPERMA INTEGRIDAD DEL ADN ESTRÉS OXIDATIVO SPARUS AURATA MOTILIDAD CRIOPROTECTOR PROTEÍNAS VIABILIDAD CALIDAD
Disciplinas de la OCDE	Pesca Biología Reproductiva Tecnologías que involucran la manipulación de Células , Tejidos, órganos o el organismo completo (Reproducción Asistida)
Título de la cita (Recomendado-único)	Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Revista/Libro	AQUACULTURE
Tipo de ruta	hibrida verde
Access Rights	metadata
Derechos de acceso	metadata
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	ANID CONICYT FONDEF DO6I1020#ANID CONICYT FONDEF D10I1064
Referencia del Financiador (Mandatado si es aplicable-repetible)	ANID CONICYT FONDEF DO6I1020 ANID CONICYT FONDEF D10I1064
Editorial	ELSEVIER
ISSN	0044-8486
Página de inicio (Recomendado-único)	119
Página final (Recomendado-único)	126
Categoría WOS	Pesca Biología Marina y de Agua Dulce Fisheries Marine & Freshwater Biology
Formato	pdf
Id de Web of Science	WOS:000312390700015