Repositorio
Institucional

Felmer, R.

Arias, M. E.

Munoz, G.

Rio, J.

Silva, M.

Berland, M.

Valdivia, R.

Kato, M.

Becker, M. I.

DeIoannes, A.

Changes in the expression pattern of developmentally important genes and possible relation to the low in vitro and in vivo developmental potential of transgenic nuclear transfer embryos

BRAZILIAN COLL ANIMAL REPRODUCTION

ANIMAL REPRODUCTION

en

The efficiency of nuclear transfer was evaluated in vitro and in vivo using non-transgenic and transgenic cloned bovine embryos. In addition, in order to better understand the low pre-and post-implantation development observed in transgenic nuclear transfer embryos, a gene expression data analysis was conducted in this group. We observed no difference (P > 0.05) in the cleavage rate of nuclear transfer embryos generated with transgenic and non-transgenic fetal fibroblasts. However, the blastocyst rate was affected (P < 0.01) in transgenic (13%) compared to non-transgenic nuclear transfer embryos (24%). Despite this difference, the quality of embryos as assessed by the total number of cells and morphological appearance was not different (P > 0.05). Pregnancy rate was also affected (P < 0.05) in transgenic (7%) compared to non-transgenic nuclear transfer embryos (43%), though all pregnancies failed to maintain to term. Gene expression data analysis revealed a decrease (P < 0.05) of INF-tau and HDAC1 in transgenic nuclear transfer embryos relative to embryos generated by in vitro fertilization. Considering the importance of these two genes in maternal recognition of pregnancy and nuclear reprogramming, the alterations observed in these transgenic embryos might help to explain the low pre-and post-implantation development observed in this group, which highlights the need to assess different donor cells before embryo transfer, particularly when the aim is to generate transgenic offspring.

Artículo original

pdf

cattle

cloning

embryo transfer

transgenic.

MESSENGER-RNA EXPRESSION

INTERFERON-TAU SECRETION

LONG-TERM CULTURE

DONOR CELL-TYPE

BOVINE EMBRYOS

FETAL FIBROBLASTS

CLONED EMBRYOS

MAMMALIAN DEVELOPMENT

GRANULOSA-CELLS

SOMATIC-CELLS

ganado vacuno

clonación

transferencia de embriones

transgénico.

EXPRESIÓN DE ARN MENSAJERO

SECRECIÓN DE INTERFERÓN-TAU

CULTIVO A LARGO PLAZO

TIPO DE CÉLULA DONANTE

EMBRIONES BOVINOS

FIBROBLASTOS FETALES

EMBRIONES CLONADOS

DESARROLLO DE MAMÍFEROS

CÉLULAS DE LA GRANULOSA

CÉLULAS SOMÁTICAS

cattle

cloning

embryo transfer

transgenic.

MESSENGER-RNA EXPRESSION

INTERFERON-TAU SECRETION

LONG-TERM CULTURE

DONOR CELL-TYPE

BOVINE EMBRYOS

FETAL FIBROBLASTS

CLONED EMBRYOS

MAMMALIAN DEVELOPMENT

GRANULOSA-CELLS

SOMATIC-CELLS

ganado vacuno

clonación

transferencia de embriones

transgénico.

EXPRESIÓN DE ARN MENSAJERO

SECRECIÓN DE INTERFERÓN-TAU

CULTIVO A LARGO PLAZO

TIPO DE CÉLULA DONANTE

EMBRIONES BOVINOS

FIBROBLASTOS FETALES

EMBRIONES CLONADOS

DESARROLLO DE MAMÍFEROS

CÉLULAS DE LA GRANULOSA

CÉLULAS SOMÁTICAS

Biotecnología agrícola

Biología del Desarrollo

Tecnologías que involucran la manipulación de Células , Tejidos, órganos o el organismo completo (Reproducción Asistida)

Changes in the expression pattern of developmentally important genes and possible relation to the low in vitro and in vivo developmental potential of transgenic nuclear transfer embryos

ANIMAL REPRODUCTION

dorada

verde

acceso abierto

acceso abierto

CC BY 4.0

en

Artículo original

versión publicada

BRAZILIAN COLL ANIMAL REPRODUCTION

1806-9614

647

656

Agricultura, Lácteos y Ciencia Animal

Biología Reproductiva

Agriculture, Dairy & Animal Science

Reproductive Biology

pdf

WOS:000334189200001