Repositorio
Institucional

Figueroa, R. G.

Lozano, E.

Bongiovanni, G.

Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device

SOC MEXICANA FISICA

REVISTA MEXICANA DE FISICA

en

Visualization of elemental distributions of biological tissue is gaining importance in many disciplines of biological, forensic, and medical research. Furthermore, the maps of elements have wide application in archeology for the understanding of the pigments, modes of preservation and environmental context. Since major advances in relation to collimators and detectors have yielded micro scale images, the chemical mapping via synchrotron scanning micro-X-ray fluorescence spectrometry (SR-mu XRF) is widely used as microanalytical techniques. However, the acquisition time is a limitation of current SR-mu XRF imaging protocols, doing tedious micro analysis of samples of more than 1 cm and very difficult to study of larger samples such as animal organ, whole organisms, work of art, etc. Recently we have developed a robotic system to image the chemistry of large specimens rapidly at concentration levels of parts per million. Multiple images of distribution of elements can be obtained on surfaces of 100x100 mm and a spatial resolution of up to 0.2 mm(2) per pixel, with a spectral capture time up to 1 ms per point. This system has proven to be highly efficient for the XRF mapping of elements in large biological samples, achieving comparables results to those obtained by SR-mu XRF. Thus, images of As and Cu accumulation in renal cortex of arsenic-exposed rats were obtained by both methodologies. However, the new imaging system enables the XRF scanning in few minutes, whereas SR-mu XRF required several hours. These and other advantages as well as the potential applications of this system, will be discussed.

Artículo original

Thanks to the National Fund for Scientific and Technological Research (FONDECYT) of Chile, which has funded this development through grant 1080306 and Morphology Unit, Department of Basic Sciences, University of La Frontera for providing bone samples used in this work. The work was supported in part by the National Fund for Scientific and Technological Research (FONCYT) PICT97-PRH33 and CONICET of Argentina.

Agradecemos al Fondo Nacional de Investigación Científica y Tecnológica (FONDECYT) de Chile, que financió este desarrollo mediante la beca 1080306, y a la Unidad de Morfología del Departamento de Ciencias Básicas de la Universidad de La Frontera por proporcionar las muestras óseas utilizadas en este trabajo. Este trabajo fue financiado en parte por el Fondo Nacional de Investigación Científica y Tecnológica (FONDECYT) PICT97-PRH33 y el CONICET de Argentina.

pdf

XRF-Imaging

biological samples

multi-elemental mapping

FLUORESCENCE

Imágenes XRF

muestras biológicas

mapeo multielemental

FLUORESCENCIA

XRF-Imaging

biological samples

multi-elemental mapping

FLUORESCENCE

Imágenes XRF

muestras biológicas

mapeo multielemental

FLUORESCENCIA

Tecnología Médica

Robótica y Sistemas de Control Automático

Anatomía y Morfología

Element distribution imaging in rat kidney using a 2D rapid scan EDXRF device

REVISTA MEXICANA DE FISICA

diamante

verde

acceso abierto

CC BY NC 3.0

en

Artículo original

versión publicada

ANID CONICYT FONDECYT 1080306#ANID CONICYT FONCYT PICT97-PRH33#CONICET

ANID CONICYT FONDECYT 1080306

ANID CONICYT FONCYT PICT97-PRH33

CONICET

SOC MEXICANA FISICA

0035-001X

292

295

Física multidisciplinaria

Physics, Multidisciplinary

pdf

WOS:000320972000002