Repositorio UFRO · Omeka S

Excess manganese differentially inhibits photosystem I versus II in Arabidopsis thaliana

Primer Autor	Alberdi, M.
Co-autores	Millaleo, R. Reyes-Diaz, M. Ivanov, A. G. Krol, M. Huener, N. P. A.
Título	Excess manganese differentially inhibits photosystem I versus II in Arabidopsis thaliana
Editorial	OXFORD UNIV PRESS
Revista	JOURNAL OF EXPERIMENTAL BOTANY
Lenguaje	en
Resumen	The effects of exposure to increasing manganese concentrations (501500 M) from the start of the experiment on the functional performance of photosystem II (PSII) and photosystem I (PSI) and photosynthetic apparatus composition of Arabidopsis thaliana were compared. In agreement with earlier studies, excess Mn caused minimal changes in the PSII photochemical efficiency measured as F-v/F-m, although the characteristic peak temperature of the S(2/3)Q(B) charge recombinations was shifted to lower temperatures at the highest Mn concentration. SDS-PAGE and immunoblot analyses also did not exhibit any significant change in the relative abundance of PSII-associated polypeptides: PSII reaction centre protein D1, Lhcb1 (major light-harvesting protein of LHCII complex), and PsbO (OEC33, a 33kDa protein of the oxygen-evolving complex). In addition, the abundance of Rubisco also did not change with Mn treatments. However, plants grown under excess Mn exhibited increased susceptibility to PSII photoinhibition. In contrast, in vivo measurements of the redox transients of PSI reaction centre (P700) showed a considerable gradual decrease in the extent of P700 photooxidation (P700) under increased Mn concentrations compared to control. This was accompanied by a slower rate of P700 re-reduction indicating a downregulation of the PSI-dependent cyclic electron flow. The abundance of PSI reaction centre polypeptides (PsaA and PsaB) in plants under the highest Mn concentration was also significantly lower compared to the control. The results demonstrate for the first time that PSI is the major target of Mn toxicity within the photosynthetic apparatus of Arabidopsis plants. The possible involvement mechanisms of Mn toxicity targeting specifically PSI are discussed.
Tipo de Recurso	Artículo original
Description	This work was supported by the Natural Sciences and Engineering Research Council of Canada (NPAH), a CONICYT internship 'Pasantias Doctorales en el Extranjero 2010', the Department of Biology and the Biotron, University of Western Ontario, London, Ontario, Canada, and the Fondecyt project, Government of Chile (no. 1080372). Este trabajo fue financiado por el Consejo de Investigación en Ciencias Naturales e Ingeniería de Canadá (NPAH), una pasantía CONICYT 'Pasantias Doctorales en el Extranjero 2010', el Departamento de Biología y el Biotrón, Universidad de Western Ontario, London, Ontario, Canadá, y el proyecto Fondecyt, Gobierno de Chile (n.º 1080372).
doi	10.1093/jxb/ers339
Formato Recurso	pdf
Palabras Claves	Chlorophyll fluorescence Mn toxicity photosystem I PSI-associated proteins PSII-associated proteins redox state of P700 PHOTOSYNTHETIC ELECTRON-TRANSPORT RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE MN TOXICITY CO2 ASSIMILATION ACCEPTOR-SIDE LIGHT PHOTOINHIBITION TOLERANCE CHLOROPHYLL RESPONSES Fluorescencia de la clorofila toxicidad del Mn fotosistema I proteínas asociadas al PSI proteínas asociadas al PSII estado redox de P700 TRANSPORTE DE ELECTRONES FOTOSINTÉTICOS RIBULOSA 1,5-BIFOSFATO CARBOXILASA TOXICIDAD DE MN ASIMILACIÓN DE CO2 LADO ACEPTOR LUZ FOTOINHIBICIÓN TOLERANCIA CLOROFILA RESPUESTAS
Ubicación del archivo	http://dx.doi.org/10.1093/jxb/ers339
Materias	Chlorophyll fluorescence Mn toxicity photosystem I PSI-associated proteins PSII-associated proteins redox state of P700 PHOTOSYNTHETIC ELECTRON-TRANSPORT RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE MN TOXICITY CO2 ASSIMILATION ACCEPTOR-SIDE LIGHT PHOTOINHIBITION TOLERANCE CHLOROPHYLL RESPONSES Fluorescencia de la clorofila toxicidad del Mn fotosistema I proteínas asociadas al PSI proteínas asociadas al PSII estado redox de P700 TRANSPORTE DE ELECTRONES FOTOSINTÉTICOS RIBULOSA 1,5-BIFOSFATO CARBOXILASA TOXICIDAD DE MN ASIMILACIÓN DE CO2 LADO ACEPTOR LUZ FOTOINHIBICIÓN TOLERANCIA CLOROFILA RESPUESTAS
Disciplinas de la OCDE	Biología Molecular Bioquímica Toxicología
Título de la cita (Recomendado-único)	Excess manganese differentially inhibits photosystem I versus II in Arabidopsis thaliana
Revista/Libro	JOURNAL OF EXPERIMENTAL BOTANY
Tipo de ruta	dorada verde
Access Rights	acceso abierto
Derechos de acceso	acceso abierto
License	CC BY NC 2.0
Condición de la licencia (Recomendado-repetible)	CC BY NC 2.0
Identificador alternativo	PMID 23183256
Idioma	en
Identificador del recurso (Mandatado-único)	Artículo original
Versión del recurso (Recomendado-único)	versión publicada
Entidad financiadora	Natural Sciences and Engineering Research Council of Canada (NPAH) University of Western Ontario, Department of Biology and the Biotron ANID CONICYT FONDECYT 1080372
Referencia del Financiador (Mandatado si es aplicable-repetible)	Natural Sciences and Engineering Research Council of Canada (NPAH) University of Western Ontario, Department of Biology and the Biotron ANID CONICYT FONDECYT 1080372
Editorial	OXFORD UNIV PRESS
ISSN	0022-0957
Página de inicio (Recomendado-único)	343
Página final (Recomendado-único)	354
Categoría WOS	Ciencias de las plantas Plant Sciences
Formato	pdf
Id de Web of Science	WOS:000312651100025